Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,492,667	G→A	V196V (GTC→GTT)	frc ←	formyl‑CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,492,667	0	G	A	100.0%	193.6 / NA	55	V196V (GTC→GTT)	frc	formyl‑CoA transferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (30/25); total (30/25)

GCGGGGAACTGCATCACCAAATGTACCATTCGGATACTGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTGACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTA  >  NC_000913/2492532‑2492799

gcgGGGAACTGCATCACCAAATGTACCATTCGGATACTGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTc                                                                                                                                  >  1:157449/1‑140 (MQ=255)

 cgGGGAACTGCATCACCAAATGTACCATTCGGATACTGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCg                                                                                                                                 >  1:689931/1‑140 (MQ=255)

 cgGGGAACTGCATCACCAAATGTACCATTCGGATACTGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCg                                                                                                                                 >  1:427292/1‑140 (MQ=255)

   gggAACTGCATCACCAAATGTACCATTCGGATACTGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGtt                                                                                                                               >  2:66227/1‑140 (MQ=255)

                       tACCATTCGGATACTGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTcg                                                                                                           <  2:330675/140‑1 (MQ=255)

                       tACCATTCGGATACTGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTcg                                                                                                           <  1:440768/140‑1 (MQ=255)

                           aTTCGGATACTGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGAt                                                                                                       >  1:249724/1‑140 (MQ=255)

                            ttCGGATACTGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATg                                                                                                      <  1:112083/140‑1 (MQ=255)

                             tCGGATACTGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATgc                                                                                                     <  2:301139/140‑1 (MQ=255)

                              cGGATACTGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTcg                                                                                                           >  1:348659/1‑133 (MQ=255)

                              cGGATACTGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTcg                                                                                                           <  2:348659/133‑1 (MQ=255)

                               ggATACTGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCTCACGCCCCGTTTTTTCGCGATgcag                                                                                                   >  1:606678/1‑140 (MQ=255)

                                gATACTGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATgcagc                                                                                                  <  2:572171/140‑1 (MQ=255)

                                 aTACTGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGTGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATgcagca                                                                                                 >  2:405998/1‑140 (MQ=255)

                                 aTACTGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATgcagca                                                                                                 <  2:725525/140‑1 (MQ=255)

                                     tGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAagc                                                                                             <  2:428460/140‑1 (MQ=255)

                                     tGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTACATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAGagc                                                                                             <  1:162746/140‑1 (MQ=255)

                                       cGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCaa                                                                                                >  2:676698/1‑135 (MQ=255)

                                       cGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCaa                                                                                                <  1:676698/135‑1 (MQ=255)

                                       cGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAagcag                                                                                           >  1:412955/1‑140 (MQ=255)

                                       cGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAagcag                                                                                           >  1:585325/1‑140 (MQ=255)

                                       cGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAagcag                                                                                           >  2:286254/1‑140 (MQ=255)

                                       cGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAagcag                                                                                           >  2:878633/1‑140 (MQ=255)

                                        gggTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAagcagc                                                                                          <  1:286254/140‑1 (MQ=255)

                                                  ccAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCg                                                                                >  1:123592/1‑140 (MQ=255)

                                                   cAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGa                                                                               <  2:412955/140‑1 (MQ=255)

                                                    aGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGAt                                                                              <  2:427292/140‑1 (MQ=255)

                                                     gATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATc                                                                             <  1:656455/140‑1 (MQ=255)

                                                        aaCCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACCTGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGc                                                                          <  1:773012/140‑1 (MQ=255)

                                                          cccAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCaa                                                                        >  1:627789/1‑140 (MQ=255)

                                                          cccAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCaa                                                                        >  1:536323/1‑140 (MQ=255)

                                                                ttATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCAt                                                                  >  1:757956/1‑140 (MQ=255)

                                                                ttATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCAACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCAt                                                                  >  1:205749/1‑140 (MQ=255)

                                                                  aTCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTc                                                                <  2:757956/140‑1 (MQ=255)

                                                                  aTCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTc                                                                >  2:444848/1‑140 (MQ=255)

                                                                  aTCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCAACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTc                                                                <  2:205749/140‑1 (MQ=255)

                                                                        aCGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGgtgt                                                          >  2:682928/1‑140 (MQ=255)

                                                                        aCGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGgtgt                                                          <  1:444848/140‑1 (MQ=255)

                                                                         cGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGtt                                                         >  2:509040/1‑140 (MQ=255)

                                                                                  tCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGTTGCTGTCAcc                                                <  1:682928/140‑1 (MQ=255)

                                                                                               cgcgGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGTTGCTGTCACCCAACGCTGCAgcg                                   <  2:157449/140‑1 (MQ=255)

                                                                                                gcgGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGTTGCTGTCACCCAACGCTGCAgcgc                                  >  1:135220/1‑140 (MQ=255)

                                                                                                gcgGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGTTGCTGTCACCCAACGCTGCAgcgc                                  >  1:685998/1‑140 (MQ=255)

                                                                                                    cAAAGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTAc                              >  2:41268/1‑140 (MQ=255)

                                                                                                     aaaGGTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTAcc                             <  2:700795/140‑1 (MQ=255)

                                                                                                         gTTCAATACGGCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAgcg                         <  1:41268/140‑1 (MQ=255)

                                                                                                                   gCATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATc               >  1:372664/1‑140 (MQ=255)

                                                                                                                   gCATCCTGCATTGACATGGTAACTAGTAGCCCACGCCCCGTTTTTTCGAGATGTAGCAAAGAAGCAAGTAAACCGATCAGCAAATGAATTCCGGTGTTGCTGTCACCCAACCCTACACCGCTTACAAGCTGCGGACCATc               >  2:279868/1‑140 (MQ=255)

                                                                                                                    cATCCTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATcc              >  1:464479/1‑140 (MQ=255)

                                                                                                                        cTGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCaaa          <  2:536323/140‑1 (MQ=255)

                                                                                                                         tGCATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCaaaa         >  1:306449/1‑140 (MQ=255)

                                                                                                                           cATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAAc       >  2:367711/1‑140 (MQ=255)

                                                                                                                           cATTGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAAc       <  2:306449/140‑1 (MQ=255)

                                                                                                                              tGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGTTGCTGTCGCCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCg    <  2:249724/140‑1 (MQ=255)

                                                                                                                              tGACATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCg    >  1:356708/1‑140 (MQ=255)

                                                                                                                                aCATGGTAACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTa  >  1:65782/1‑140 (MQ=255)

GCGGGGAACTGCATCACCAAATGTACCATTCGGATACTGCGGGTATTCTTCCAGATAACCCAATTTATCGAGACGCTGCTGGTCACGTAATTTCACGCGGCAAAGGTTCAATACGGCATCCTGCATTGACATGGTGACTCGTTGCCCACGCCCCGTTTTTTCGCGATGCAGCAAAGCAGCAAGTAAACCGATCAGCAAATGCATTCCGGTGTTGCTGTCACCCAACGCTGCAGCGCTTACCAGCGGCGGACCATCCCAAAAACCCGTA  >  NC_000913/2492532‑2492799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A