Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,550,910	T→C	T103A (ACG→GCG)	yfeY ←	DUF1131 domain‑containing lipoprotein YfeY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,550,910	0	T	C	100.0%	104.7 / NA	30	T103A (ACG→GCG)	yfeY	DUF1131 domain‑containing lipoprotein YfeY
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/16); total (14/16)

GATTATCATCACCATCAGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCGGCAATGGCT  >  NC_000913/2550781‑2551043

gATTATCATCACCATCAGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCg                                                                                                                             <  1:478738/140‑1 (MQ=255)

       atcaCCATCAGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTaa                                                                                                                      >  2:288835/1‑140 (MQ=255)

           ccATCAGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCAc                                                                                                                  <  2:745656/140‑1 (MQ=255)

           ccATCAGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCAc                                                                                                                  <  2:548890/140‑1 (MQ=255)

               cAGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATc                                                                                                              >  1:639114/1‑140 (MQ=255)

                aGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCg                                                                                                             >  1:343678/1‑140 (MQ=255)

                   tttttGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCa                                                                                                          <  1:475083/140‑1 (MQ=255)

                         gCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGt                                                                                                    <  1:195515/140‑1 (MQ=255)

                         gCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGt                                                                                                    <  1:753956/140‑1 (MQ=255)

                            aTTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCAc                                                                                                                  >  1:316559/1‑123 (MQ=255)

                            aTTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCAc                                                                                                                  <  2:316559/123‑1 (MQ=255)

                            aTTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGc                                                                                                 <  1:187075/140‑1 (MQ=255)

                             ttGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGcc                                                                                                <  2:85795/140‑1 (MQ=255)

                                   aaTGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTtcat                                                                                          <  1:288835/140‑1 (MQ=255)

                                                                       aTTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCGTCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGt                                                      <  2:639114/140‑1 (MQ=255)

                                                                       aTTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGt                                                      >  1:140877/1‑140 (MQ=255)

                                                                            aaCACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCa                                                 >  1:878317/1‑140 (MQ=255)

                                                                                  ggTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGc                                           >  1:756638/1‑140 (MQ=255)

                                                                                  ggTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGc                                           >  2:171163/1‑140 (MQ=255)

                                                                                   gtgtCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCt                                          >  1:448597/1‑140 (MQ=255)

                                                                                      tCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTgcg                                       <  2:343678/140‑1 (MQ=255)

                                                                                                  tCGCTATCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATc                           >  1:649243/1‑140 (MQ=255)

                                                                                                        tCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATc                     >  2:74737/1‑140 (MQ=255)

                                                                                                        tCCAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATc                     >  2:732369/1‑140 (MQ=255)

                                                                                                         ccAGCACGTCAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCa                    <  1:171163/140‑1 (MQ=255)

                                                                                                                  cAATGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCg           >  1:87225/1‑140 (MQ=255)

                                                                                                                     tGCGACTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCGGCa        <  2:649243/140‑1 (MQ=255)

                                                                                                                          cTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTGGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCGGCa        >  2:786873/1‑135 (MQ=255)

                                                                                                                          cTGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCGGCa        <  1:786873/135‑1 (MQ=255)

                                                                                                                           tGATCGCGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCGGCAATGGCt  <  2:87225/140‑1 (MQ=255)

GATTATCATCACCATCAGCTTTTTGGCAATTGCCAAATGCTTTGCTGTAAAGGTCGCTAAACGGTGTACCGATTTTAACACCGGTGTCAGCAGGAATATCGCTATCCAGCACGTCAATGCGACTGATCGTGCCTTGATCGCCGTTAATCACCATCGCCACGTTGTCGCCCTTCATCACTTCAAAAAAGCGCACCACGTTGCCGTTCGCGGTTTTCATTCCGCTGCGCAGGCGATAATCGCCATCAAGCGCATCGGCAATGGCT  >  NC_000913/2550781‑2551043

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A