Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,614,706	T→C	M258T (ATG→ACG)	focB →	putative formate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,614,706	0	T	C	100.0%	108.6 / NA	32	M258T (ATG→ACG)	focB	putative formate transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (9/23); total (9/23)

TTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTATCGGGCTATTTATTTACGTCAGGAACCCTGAAAAATCAGCCCGGCGAAACAGTTCGTCGGGCTGAAGATTATTCTTGCGCGATTTGCCCAT  >  NC_000913/2614570‑2614842

ttgtttgtGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCt                                                                                                                                       >  1:319522/1‑140 (MQ=255)

ttgtttgtGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCt                                                                                                                                       <  1:780211/140‑1 (MQ=255)

    ttgtGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGt                                                                                                                                   <  1:142199/140‑1 (MQ=255)

         aTTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAatat                                                                                                                              >  1:818386/1‑140 (MQ=255)

               ttCGCCATTGCCATTCGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATcgg                                                                                                                        >  1:556230/1‑140 (MQ=255)

               ttCCCCTTTGCCTTTCGCCATTTCCCCCCCCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATcgg                                                                                                                        <  2:873278/140‑1 (MQ=255)

                 cGCCATTGCCATTCGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATcggcg                                                                                                                      >  2:495533/1‑140 (MQ=255)

                    cattgccattCGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGgcggtg                                                                                                                   <  2:353744/140‑1 (MQ=255)

                      ttgccattCGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGgcggtgcg                                                                                                                 <  1:495533/140‑1 (MQ=255)

                        gccattCGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCCCTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGgcggtgcggt                                                                                                               <  2:556230/140‑1 (MQ=255)

                         ccattCGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGcggtgcggtg                                                                                                              <  2:319522/140‑1 (MQ=255)

                            ttCGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTg                                                                                                           <  2:9837/140‑1 (MQ=255)

                              cGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGt                                                                                                         <  2:818386/140‑1 (MQ=255)

                              cGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGt                                                                                                         <  2:298410/140‑1 (MQ=255)

                                  aTTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGt                                                                                                     <  1:436216/140‑1 (MQ=255)

                                   tttCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGt                                                                                                         >  2:421844/1‑135 (MQ=255)

                                   tttCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGt                                                                                                         <  1:421844/135‑1 (MQ=255)

                                    ttCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGGGGGGCTgggggggat                                                                                                   >  2:349879/1‑133 (MQ=255)

                                               ccccTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTATCggg                                                                                        <  1:349879/140‑1 (MQ=255)

                                               ccccTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTATCggg                                                                                        <  1:330430/140‑1 (MQ=255)

                                               ccccTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTCATCggg                                                                                        <  2:233487/140‑1 (MQ=255)

                                                 ccTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCTTTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTATCGGGCt                                                                                      <  2:56712/140‑1 (MQ=255)

                                                   ttCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTATCGGGCtat                                                                                    <  1:778064/140‑1 (MQ=255)

                                                     cTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTATCGGGCtattt                                                                                  >  1:542864/1‑140 (MQ=255)

                                                     cTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTATCGGGCtattt                                                                                  >  1:2556/1‑140 (MQ=255)

                                                            cTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTATCGGGCTATTTATTTACg                                                                           >  2:526315/1‑140 (MQ=255)

                                                                cgcACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTATCGGGCTATTTATTTACGTCAg                                                                       <  2:2556/140‑1 (MQ=255)

                                                                cgcACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTATCGGGCTATTTATTTACGTCAg                                                                       <  2:542864/140‑1 (MQ=255)

                                                                        aGCGCAGACATTTTTCCGGCATTGACTGTCAGCCATTTTATTCCCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTATCGGGCTATTTATTTACGTCAGGAACCCTg                                                               <  1:467757/140‑1 (MQ=255)

                                                                                        cGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTATCGGGCTATTTATTTACGTCAGGAACCCTGAAAAATCAGCCCGGCg                                               <  1:574781/140‑1 (MQ=255)

                                                                                             cTGACGGTCAGCAATTTTATTGCCGCCAATCTGCTCCCGGTGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTATCGGGCTATTTATTTACGTCAGGAACCCTGAAAAATCAGCCCGGCGAAACa                                          <  1:758279/140‑1 (MQ=255)

                                                                                                                                     tGACGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTATCGGGCTATTTATTTACGTCAGGAACCCTGAAAAATCAGCCCGGCGAAACAGTTCGTCGGGCTGAAGATTATTCTTGCGCGATTTGCCCAt  <  2:365115/140‑1 (MQ=255)

TTGTTTGTGATTCCGTTCGCCATTGCCATTCGCCATTTCGCCCCTCCCCCCTTCTGGCAGCTGGCGCACAGTAGCGCAGACAATTTTCCGGCACTGACGGTCAGCCATTTTATTACCGCCAATCTGCTCCCGGTGATGCTGGGTAATATTATCGGCGGTGCGGTGCTGGTGAGTATGTGTTATCGGGCTATTTATTTACGTCAGGAACCCTGAAAAATCAGCCCGGCGAAACAGTTCGTCGGGCTGAAGATTATTCTTGCGCGATTTGCCCAT  >  NC_000913/2614570‑2614842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A