Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,615,702	T→C	Q60Q (CAA→CAG)	yfgO ←	putative transporter YfgO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,615,702	0	T	C	100.0%	71.5 / NA	22	Q60Q (CAA→CAG)	yfgO	putative transporter YfgO
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (7/15); total (7/15)

CATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAAT  >  NC_000913/2615573‑2615827

cATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCa                                                                                                                     <  2:186691/140‑1 (MQ=255)

   atCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACgg                                                                                                                  <  1:147410/140‑1 (MQ=255)

    tCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCCCggg                                                                                                                 >  2:59399/1‑139 (MQ=255)

    tCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCACCCAATAGACTGCAGAAACACggg                                                                                                                 >  2:387286/1‑139 (MQ=255)

              aGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTcc                                                                                                       >  2:692262/1‑140 (MQ=255)

                    tGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCaaa                                                                                                 >  1:670861/1‑140 (MQ=255)

                       ccTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGATGCACGGTTGGCCATTCCAGCAAATAg                                                                                              <  1:59399/140‑1 (MQ=255)

                        cTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAAAAgg                                                                                             >  2:391271/1‑140 (MQ=255)

                                aGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGcaca                                                                                     <  1:204344/140‑1 (MQ=255)

                                 ggCGATAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACaa                                                                                    >  1:806537/1‑140 (MQ=255)

                                   cGATAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATa                                                                                  >  1:881016/1‑140 (MQ=255)

                                   cGATAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATa                                                                                  <  1:600098/140‑1 (MQ=255)

                                     aTAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATa                                                                                  >  2:472709/1‑138 (MQ=255)

                                     aTAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATa                                                                                  <  1:472709/138‑1 (MQ=255)

                                     aTAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAAAAAACTGCAGACGCACCGGTGGCCATTCCAGCAAATAGGCCAGCACaaaaac                                                                                >  2:600098/1‑136 (MQ=255)

                                      tAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGcc                                                                               <  2:375338/140‑1 (MQ=255)

                                             tACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGGCAAAAGACGGCAGACGCACGGGTGGGCATTTCAGCAAAAAGGCCAGCACAATAACCACCAGCa                                                                        >  2:420900/1‑140 (MQ=255)

                                             tACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCa                                                                        <  1:633466/140‑1 (MQ=255)

                                             tACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCa                                                                        <  1:467909/140‑1 (MQ=255)

                                                cACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGca                                                                                       >  1:644445/1‑122 (MQ=255)

                                                cACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGca                                                                                       <  2:644445/122‑1 (MQ=255)

                                                       gCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGagcaag                                                              <  2:396917/140‑1 (MQ=255)

                                                       gCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAAACTGCAAACGCACGGTTGGCCATTCCCGCAAAAAAGCCAGCACAAAAACCACCAGCAACGGagcaaa                                                              >  2:607885/1‑139 (MQ=255)

                                                         cATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGagcaagca                                                            <  2:216388/140‑1 (MQ=255)

                                                                         ccGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGaaaaa                                            >  1:703262/1‑140 (MQ=255)

                                                                            acgaAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGAt                                         <  2:670861/140‑1 (MQ=255)

                                                                             cgaAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATa                                        >  1:313005/1‑140 (MQ=255)

                                                                                         aTACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCg                            <  1:692262/140‑1 (MQ=255)

                                                                                                  aCGTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAAAAGGCCAGCACAAAAGCCACCCGCAACGGGGGAAACAGGCCACTAAAAAAAAAAAAAAAGCCAAATACGGCAACTaaa                   >  2:224781/1‑140 (MQ=255)

                                                                                                   cGTCGCCCAGCGGCGGGAGCCGGCAATAAACTAAAAACCCACCGTTGGCCATTCCCGCGAAAAAGCCCGCCCAAAAGCCACCAGCAACGGCGCAAGGCGGGCCCCAAAGAAAAAGATAATAGCTAATACGGCAACCaaaa                  >  2:142752/1‑140 (MQ=255)

                                                                                                    gTCGCCCAGCGGCGGGAGCAGCCAATAGACTGCAGACGCACGGGTGGCCATTCCAGCAAAAAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAAATAAAGCCAAATCCGGCAACTaaaaa                 >  1:264390/1‑139 (MQ=255)

                                                                                                                   gAGCAGCCAATAGACTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAAt  <  2:881016/140‑1 (MQ=255)

CATATCGCGGATTAAGTAGATGCCCTGTTGCCAGGCGATAGGCAGTACCACGAACGCCATCAGTAGCAATATACCGACGAAAACCACCAATACAATCGACGTCGCCCAGCGGCGGGAGCAGCCAATAGATTGCAGACGCACGGTTGGCCATTCCAGCAAATAGGCCAGCACAATAGCCACCAGCAACGGAGCAAGCAGGCCACTAAAGAAAAAGATAATGCCAAATCCGGCAACTAAAATAACCAGCAAGGCAAT  >  NC_000913/2615573‑2615827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A