Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,624,731	T→C	V563A (GTT→GCT)	ppk →	polyphosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,624,731	0	T	C	100.0%	198.2 / NA	56	V563A (GTT→GCT)	ppk	polyphosphate kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (29/27); total (29/27)

TGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGTTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTAC  >  NC_000913/2624596‑2624864

tggACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTcgc                                                                                                                                   >  2:561669/1‑140 (MQ=255)

tggACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTcgc                                                                                                                                   >  1:878357/1‑140 (MQ=255)

  gACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCgg                                                                                                                                 >  2:489417/1‑140 (MQ=255)

   aCCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGa                                                                                                                                <  1:66243/140‑1 (MQ=255)

     cgcgAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAAt                                                                                                                              >  2:268521/1‑140 (MQ=255)

       cgAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGaa                                                                                                                               <  1:106174/137‑1 (MQ=255)

       cgAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGaa                                                                                                                               >  2:106174/1‑137 (MQ=255)

       cgAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAAtgt                                                                                                                            >  2:706813/1‑140 (MQ=255)

       cgAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAAtgt                                                                                                                            >  2:520857/1‑140 (MQ=255)

                 aaCGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGAt                                                                                                                  <  1:268521/140‑1 (MQ=255)

                   cgcgCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATcc                                                                                                                <  1:85890/140‑1 (MQ=255)

                   cgcgCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATcc                                                                                                                <  1:510835/140‑1 (MQ=255)

                          caAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCt                                                                                                                     <  2:95447/128‑1 (MQ=255)

                          caAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCt                                                                                                                     >  1:95447/1‑128 (MQ=255)

                              ggCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGaa                                                                                                     >  2:370861/1‑140 (MQ=255)

                                    ccAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCAtt                                                                                               >  1:435968/1‑140 (MQ=255)

                                       gTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGc                                                                                            <  2:878357/140‑1 (MQ=255)

                                        tGGTGTCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCg                                                                                           >  2:282316/1‑140 (MQ=255)

                                        tGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCg                                                                                           >  2:776796/1‑140 (MQ=255)

                                              cACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGacaaca                                                                                     <  1:520857/140‑1 (MQ=255)

                                                cccTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACAtt                                                                                   >  2:506925/1‑140 (MQ=255)

                                                   tGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGt                                                                                >  2:42358/1‑140 (MQ=255)

                                                   tGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGt                                                                                <  1:506925/140‑1 (MQ=255)

                                                   tGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGt                                                                                >  2:26383/1‑140 (MQ=255)

                                                   tGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGt                                                                                <  1:370861/140‑1 (MQ=255)

                                                     aaGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGc                                                                              <  1:282316/140‑1 (MQ=255)

                                                      aGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGcc                                                                             <  1:489417/140‑1 (MQ=255)

                                                      aGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGcc                                                                             <  1:662450/140‑1 (MQ=255)

                                                        cTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCAt                                                                           <  1:629792/140‑1 (MQ=255)

                                                          aaaTAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCa                                                                         <  1:26383/140‑1 (MQ=255)

                                                                 cTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTAttgt                                                                  >  1:168379/1‑140 (MQ=255)

                                                                  ttGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTAttgtt                                                                 >  2:691262/1‑140 (MQ=255)

                                                                   tGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTAttg                                                                   <  1:122372/137‑1 (MQ=255)

                                                                   tGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTAttg                                                                   >  2:122372/1‑137 (MQ=255)

                                                                     tCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGAc                                                              <  1:504717/140‑1 (MQ=255)

                                                                     tCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGAc                                                              >  2:44144/1‑140 (MQ=255)

                                                                               ccTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTg                                                    >  1:713388/1‑140 (MQ=255)

                                                                                 tGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCTGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGaa                                                  <  1:61398/140‑1 (MQ=255)

                                                                                   gTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACa                                                >  2:806554/1‑140 (MQ=255)

                                                                                        tCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCTCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGAcc                                           >  1:378112/1‑140 (MQ=255)

                                                                                                  gCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTatat                                 >  2:145328/1‑140 (MQ=255)

                                                                                                      ccTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGAc                                            >  1:241354/1‑125 (MQ=255)

                                                                                                      ccTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGAc                                            <  2:241354/125‑1 (MQ=255)

                                                                                                      ccTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATAttttt                             >  2:530450/1‑140 (MQ=255)

                                                                                                      ccTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATAttttt                             >  2:78924/1‑140 (MQ=255)

                                                                                                       cTCCAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTg                            >  1:626891/1‑140 (MQ=255)

                                                                                                         ccAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGaa                          >  2:757962/1‑140 (MQ=255)

                                                                                                          cAGCTCCGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGaaa                         <  2:168379/140‑1 (MQ=255)

                                                                                                                cGGCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATggcg                   >  1:723009/1‑140 (MQ=255)

                                                                                                                 ggCGTACCGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATggcgg                  <  1:806554/140‑1 (MQ=255)

                                                                                                                        cGGTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATaaa           <  1:691262/140‑1 (MQ=255)

                                                                                                                         ggTTAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATaaaa          <  2:713388/140‑1 (MQ=255)

                                                                                                                           ttAATCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAg        <  1:530450/140‑1 (MQ=255)

                                                                                                                              aTCTGCTGGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTc     <  1:78924/140‑1 (MQ=255)

                                                                                                                                 tgctgGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTAc  <  1:706813/140‑1 (MQ=255)

                                                                                                                                    tgGCTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTc     <  1:619999/134‑1 (MQ=255)

TGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTCGATAAAGGCCTGGTTGATCGTCTGTATGCGGCCTCCAGCTCCGGCGTACCGGTTAATCTGCTGGTTCGCGGAATGTGTTCGCTGATCCCCAATCTGGAAGGCATTAGCGACAACATTCGTGCCATCAGTATTGTTGACCGTTACCTTGAACATGACCGGGTTTATATTTTTGAAAATGGCGGCGATAAAAAGGTCTAC  >  NC_000913/2624596‑2624864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A