Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,625,117	G→A	M1M (ATG→ATA) †	ppx →	exopolyphosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,625,117	0	G	A	100.0%	157.5 / NA	48	M1M (ATG→ATA) †	ppx	exopolyphosphatase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (24/24); total (24/24)

CGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATGCCAATACACGATAAATCCCCTCGTCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCAT  >  NC_000913/2624981‑2625252

cGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCa                                                                                                                                      >  1:59527/1‑140 (MQ=255)

cGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCa                                                                                                                                      >  1:530423/1‑140 (MQ=255)

          aaaGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATa                                                                                                                            >  1:416822/1‑140 (MQ=255)

                cGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGGACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATccc                                                                                                                      >  2:644606/1‑140 (MQ=255)

                 gTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATcccc                                                                                                                     >  2:75886/1‑140 (MQ=255)

                  ttATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCt                                                                                                                    >  1:630646/1‑140 (MQ=255)

                     tatCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCgc                                                                                                                 >  1:624965/1‑139 (MQ=255)

                      atCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCgcc                                                                                                                >  1:854452/1‑138 (MQ=255)

                        cGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCg                                                                                                              <  2:530423/140‑1 (MQ=255)

                           tAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAg                                                                                                           >  2:831577/1‑140 (MQ=255)

                           tAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAg                                                                                                           >  1:638584/1‑140 (MQ=255)

                               gAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGt                                                                                                       >  2:61194/1‑140 (MQ=255)

                                         aTCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGt                                                                                             <  1:825017/140‑1 (MQ=255)

                                          tCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTc                                                                                            >  2:280752/1‑140 (MQ=255)

                                                       cgcgGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCaa                                                                               >  2:151892/1‑140 (MQ=255)

                                                            cAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGt                                                                          <  2:630646/140‑1 (MQ=255)

                                                                   cgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTcaca                                                                   <  2:661005/140‑1 (MQ=255)

                                                                   cgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTcaca                                                                   <  2:158365/140‑1 (MQ=255)

                                                                   cgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTcaca                                                                   <  1:95335/140‑1 (MQ=255)

                                                                   cgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTcaca                                                                   <  2:507874/140‑1 (MQ=255)

                                                                   cgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTcaca                                                                   <  2:624965/140‑1 (MQ=255)

                                                                   cgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTcaca                                                                   <  1:280752/140‑1 (MQ=255)

                                                                   cgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTcaca                                                                   <  1:396257/140‑1 (MQ=255)

                                                                   cgcAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTcaca                                                                   <  1:551984/140‑1 (MQ=255)

                                                                               gcgcAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGccc                                                       <  1:237137/140‑1 (MQ=255)

                                                                               gcgcAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGccc                                                       <  1:78895/140‑1 (MQ=255)

                                                                               gcgcAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGccc                                                       <  1:61194/140‑1 (MQ=255)

                                                                                cgcAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCg                                                      <  2:854452/140‑1 (MQ=255)

                                                                                 gcAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCgt                                                     <  1:550302/140‑1 (MQ=255)

                                                                                  cAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGTTTCAAACAGTTTTCACATGGTCATAGccc                                                       <  1:687772/137‑1 (MQ=255)

                                                                                  cAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGTTTCAAACAGTTTTCACATGGTCATAGccc                                                       >  2:687772/1‑137 (MQ=255)

                                                                                  cAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCgtg                                                    <  2:207500/140‑1 (MQ=255)

                                                                                  cAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCgtg                                                    >  1:163076/1‑140 (MQ=255)

                                                                                    gTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTcaca                                                                   >  2:214251/1‑123 (MQ=255)

                                                                                    gTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTcaca                                                                   <  1:214251/123‑1 (MQ=255)

                                                                                           aTTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCGTGTGGTAGATg                                           <  2:416822/140‑1 (MQ=255)

                                                                                                      cATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCGTGTGGTAGATGGTg                                        >  1:780573/1‑132 (MQ=255)

                                                                                                      cATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCGTGTGGTAGATGGTg                                        <  2:780573/132‑1 (MQ=255)

                                                                                                      cATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAg                                >  1:158897/1‑140 (MQ=255)

                                                                                                      cATCAAATCACTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAg                                >  2:423767/1‑140 (MQ=255)

                                                                                                                cTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCa                                 <  1:516581/129‑1 (MQ=255)

                                                                                                                cTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCa                                 >  2:516581/1‑129 (MQ=255)

                                                                                                                cTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGgcc                      >  1:611322/1‑140 (MQ=255)

                                                                                                                cTCGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGgcc                      >  1:184021/1‑140 (MQ=255)

                                                                                                                  cGAACAACCTGAATAACCCTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGgccgc                    <  2:611322/140‑1 (MQ=255)

                                                                                                                                   ccTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCa   >  1:588481/1‑140 (MQ=255)

                                                                                                                                   ccTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCa   >  2:11048/1‑140 (MQ=255)

                                                                                                                                    cTATACCAATACACGATAAATCCCCTCGCCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCAt  <  1:423767/140‑1 (MQ=255)

CGATACGGTCAAAGCACGTTATATCGATAAAGAACTCAGTAATCGCTACGTTCCCCGCGGCAATCGCCGCAAAGTACGGGCGCAGTTGGCGATTTATGACTACATCAAATCACTCGAACAACCTGAATAACCCTATGCCAATACACGATAAATCCCCTCGTCCGCAGGAGTTTGCTGCGGTCGATCTTGGTTCAAACAGTTTTCACATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCAT  >  NC_000913/2624981‑2625252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A