Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,644,561	T→C	T102A (ACT→GCT)	ndk ←	nucleoside diphosphate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,644,561	0	T	C	100.0%	113.8 / NA	34	T102A (ACT→GCT)	ndk	nucleoside diphosphate kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/17); total (17/17)

ATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCAAAGAAATAAGCGATTTCGCGAGCGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGTACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTT  >  NC_000913/2644432‑2644695

attaACGGGTGCGCGGGCACACTTCGCCTTCGCCAAAGAAATAAGCGATTTCGCGAGCGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGt                                                                                                                              <  2:411417/140‑1 (MQ=255)

         tGCGCGGGCACACTTCGCCTTCGCCAAAGAAATAAGCGATTTCGCGAGCGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGcc                                                                                                                     >  1:361469/1‑140 (MQ=255)

         tGCGCGGGCACACTTCGCCTTCGCCAAAGAAATAAGCGATTTCGCGAGCGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGcc                                                                                                                     >  2:754699/1‑140 (MQ=255)

           cgcgGGCACACTTCGCCTTCGCCAAAGAAATAAGCGATTTCGCGAGCGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCgg                                                                                                                   >  1:91645/1‑140 (MQ=255)

             cgGGCACACTTCGCCTTCGCCAAAGAAATAAGCGATTTCGCGAGCGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGAt                                                                                                                 <  1:173149/140‑1 (MQ=255)

                  acacTTCGCCTTCGCCAAAGAAATAAGCGATTTCGCGAGCGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTc                                                                                                            <  2:361469/140‑1 (MQ=255)

                      ttcgccttcgccAAAGAAATAAGCGATTTCGCGAGCGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCgcgc                                                                                                        <  2:77313/140‑1 (MQ=255)

                        cgccttcgccAAAGAAATAAGCGATTTCGCGAGCGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGccc                                                                                                      >  2:751867/1‑140 (MQ=255)

                          ccttcgccAAAGAAATAAGCGATTTCGCGAGCGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCcag                                                                                                    >  2:721082/1‑140 (MQ=255)

                            ttcgccAAAGAAATAAGCGATTTCGCGAGCGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCcagca                                                                                                  <  1:684854/140‑1 (MQ=255)

                              cgccAAAGAAATAAGCGATTTCGCGAGCGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGa                                                                                                >  2:91763/1‑140 (MQ=255)

                              cgccAAAGAAATAAGCGATTTCGCGAGCGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGa                                                                                                >  1:492281/1‑140 (MQ=255)

                              cgccAAAGAAATAAGCGATTTCGCGAGCGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGa                                                                                                >  2:598846/1‑140 (MQ=255)

                                    aGAAATAAGCGATTTCGCGAGCGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCgg                                                                                          >  2:108778/1‑140 (MQ=255)

                                         tAAGCGATTTCGCGAGCGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACg                                                                                     >  2:163209/1‑140 (MQ=255)

                                                tttCGCGAGCGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACg                                                                              <  2:91645/140‑1 (MQ=255)

                                                         cGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCa                                                                     >  2:95919/1‑140 (MQ=255)

                                                                ttCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCa                                                              <  1:751867/140‑1 (MQ=255)

                                                                     cGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACg                                                         <  2:492281/140‑1 (MQ=255)

                                                                     cGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACg                                                         <  2:546434/140‑1 (MQ=255)

                                                                      ggAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACgg                                                        <  1:95919/140‑1 (MQ=255)

                                                                                ccGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGAt                                              <  1:309747/140‑1 (MQ=255)

                                                                                ccGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGAt                                              <  1:617281/140‑1 (MQ=255)

                                                                                   tGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCgg                                           >  2:834955/1‑140 (MQ=255)

                                                                                        aCCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCag                                      <  1:91763/140‑1 (MQ=255)

                                                                                        aCCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCag                                      <  1:130121/140‑1 (MQ=255)

                                                                                          cGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCagag                                    >  2:826067/1‑140 (MQ=255)

                                                                                                    cAGGCTGTCAGCGTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAAtt                          <  1:754699/140‑1 (MQ=255)

                                                                                                                gTAATCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCAt              <  1:628646/140‑1 (MQ=255)

                                                                                                                  aaTCAGCGCGCAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCa            >  2:849647/1‑140 (MQ=255)

                                                                                                                       gcgcgcAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGaa       <  1:826067/140‑1 (MQ=255)

                                                                                                                         gcgcAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACg     >  2:580843/1‑140 (MQ=255)

                                                                                                                         gcgcAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACg     >  1:244162/1‑140 (MQ=255)

                                                                                                                            cAGAGCACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGtt  >  1:469576/1‑140 (MQ=255)

ATTAACGGGTGCGCGGGCACACTTCGCCTTCGCCAAAGAAATAAGCGATTTCGCGAGCGGCAGATTCGACGGAATCAGAACCGTGGGTACCGTTTTCGGTCAGGCTGTCAGCGTAATCAGCGCGCAGAGTACCAGCCAGTGCGTTTGCCGGATTGGTCGCGCCCAGCAGATCGCGGTGACGCTGAACGGCGTTTTCACCTTCCAGCACGGAAACCACGATCGGGCCAGAGGTCATGAATTCAACCAGACCATCAAAGAACGGTT  >  NC_000913/2644432‑2644695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A