Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,645,282	G→A	P682S (CCG→TCG)	pbpC ←	peptidoglycan glycosyltransferase PbpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,645,282	0	G	A	100.0%	96.9 / NA	28	P682S (CCG→TCG)	pbpC	peptidoglycan glycosyltransferase PbpC
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (14/14); total (14/14)

CCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTATCATTTCTTGTCGCGCTTGCGGGCAATCAGCGGCAACACGTTTGCCAT  >  NC_000913/2645145‑2645416

ccaccaGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGc                                                                                                                                      >  1:844961/1‑140 (MQ=255)

ccaccaGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGc                                                                                                                                      >  1:745524/1‑140 (MQ=255)

  accaGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCaa                                                                                                                                    >  1:848194/1‑140 (MQ=255)

             ccTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGc                                                                                                                         <  2:844961/140‑1 (MQ=255)

                 ccccTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCggt                                                                                                                     >  1:123242/1‑140 (MQ=255)

                 ccccTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCggt                                                                                                                     >  1:8593/1‑140 (MQ=255)

                      ccACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCGGTGGTaa                                                                                                                >  1:861256/1‑140 (MQ=255)

                        aCTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCGGTGGTAAgc                                                                                                              >  2:530811/1‑140 (MQ=255)

                           tGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCGGTGGTAAGCGca                                                                                                           <  1:291076/140‑1 (MQ=255)

                           tGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCGGTGGTAAGCGca                                                                                                           >  2:318895/1‑140 (MQ=255)

                            gATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCGGTGGTAAGCGcac                                                                                                          >  2:833698/1‑140 (MQ=255)

                             aTTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCggtggt                                                                                                                  <  1:236322/131‑1 (MQ=255)

                             aTTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCggtggt                                                                                                                  >  2:236322/1‑131 (MQ=255)

                                gCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCGGTGGTAAGCGCACAgcg                                                                                                      <  2:485005/140‑1 (MQ=255)

                                 cAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCGGTGGTAAGCGCACAgcgc                                                                                                     >  2:1639/1‑140 (MQ=255)

                                  aaCGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGcgcg                                                                                                    <  1:530811/140‑1 (MQ=255)

                                       cAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGtt                                                                                               >  2:51727/1‑140 (MQ=255)

                                                     cTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGc                                                                                 <  2:8593/140‑1 (MQ=255)

                                                                           aatCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGcc                                                           <  2:101825/140‑1 (MQ=255)

                                                                           aatCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGcc                                                           <  2:348222/140‑1 (MQ=255)

                                                                           aatCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGcc                                                           <  2:848194/140‑1 (MQ=255)

                                                                                cgcCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACa                                                      <  1:800591/140‑1 (MQ=255)

                                                                                 gcCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACAt                                                     <  1:833698/140‑1 (MQ=255)

                                                                                    aTCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTa                                                  <  1:1639/140‑1 (MQ=255)

                                                                                               cTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTATCATTTCTTGt                                       <  2:861256/140‑1 (MQ=255)

                                                                                                         cAGCGGGAGCTGGGCATCGTGACCGTATGGCGAGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTATCATTTCTTGTCGCGCTTGCg                             <  2:123242/140‑1 (MQ=255)

                                                                                                                                  tATGGCGAGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTATCATTTCTTGTCGCGCTTGCGGGCAATCAGCGGCAACACGTTTGcc    >  1:537166/1‑140 (MQ=255)

                                                                                                                                    tGGCGAGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTATCATTTCTTGTCGCGCTTGCGGGCAATCAGCGGCAACACGTTTGCCAt  >  1:202732/1‑140 (MQ=255)

CCACCAGCGTTCACCTGCCCCTCCACTTGATTGCAACGGCAAAGTCGCTTCCGCTGCGCCCGGTAAACGTTTAATAATCGCGCCATCGCGAACGCCTGTCAGCTGCAGCGGGAGCTGGGCATCGTGACCGTATGGCGGGCAACTTGTCGAGGCCGGTGGTAAGCGCACAGCGCGACGTTCTGATGCGGGCAGCCAGGGTTCCAGCGGTAGCGGCCAGACATTTATCATTTCTTGTCGCGCTTGCGGGCAATCAGCGGCAACACGTTTGCCAT  >  NC_000913/2645145‑2645416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A