Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,738,583	T→C	F280S (TTT→TCT)	pheA →	fused chorismate mutase/prephenate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,738,583	0	T	C	100.0%	165.0 / NA	47	F280S (TTT→TCT)	pheA	fused chorismate mutase/prephenate dehydratase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/24); total (23/24)

GCGGCAATGGAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATT  >  NC_000913/2738450‑2738716

gCGGCAATGGAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTgtggt                                                                                                                                 >  1:315101/1‑140 (MQ=255)

 cGGCAATGGAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTgtggtg                                                                                                                                <  2:625564/140‑1 (MQ=255)

   gCAATGGAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGtt                                                                                                                              >  1:589892/1‑140 (MQ=255)

    cAATGGAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTg                                                                                                                             <  2:750364/140‑1 (MQ=255)

    cAATGGAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTg                                                                                                                             <  2:452200/140‑1 (MQ=255)

     aaTGGAAAAGGTTGCACAGGCAAAATCACCGTATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTgg                                                                                                                            <  2:87170/140‑1 (MQ=255)

     aaTGGAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTgg                                                                                                                            <  2:285346/140‑1 (MQ=255)

         gAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGcgcg                                                                                                                        <  2:785641/140‑1 (MQ=255)

                 tGCACAGGCAAAATCACCGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCa                                                                                                                >  1:685256/1‑140 (MQ=255)

                   cacaGGCAAAATCACCGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCAtt                                                                                                              >  2:696068/1‑140 (MQ=255)

                        gCAAAATCACCGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACgt                                                                                                         >  1:232403/1‑140 (MQ=255)

                          aaaaTCACCGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACgtgt                                                                                                       <  1:696068/140‑1 (MQ=255)

                                  cGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAg                                                                                               >  1:478004/1‑140 (MQ=255)

                                  cGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAg                                                                                               >  1:502527/1‑140 (MQ=255)

                                     aTGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGtt                                                                                            <  1:186020/140‑1 (MQ=255)

                                      tGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTc                                                                                           <  2:232403/140‑1 (MQ=255)

                                               gTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGaaaa                                                                                  >  1:733053/1‑140 (MQ=255)

                                               gTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGaaaa                                                                                  >  1:621566/1‑140 (MQ=255)

                                                 tGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAAcc                                                                                <  2:478004/140‑1 (MQ=255)

                                                  gggAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCa                                                                               <  2:685256/140‑1 (MQ=255)

                                                   ggAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCAc                                                                              <  1:306992/140‑1 (MQ=255)

                                                   ggAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCAc                                                                              >  1:550252/1‑140 (MQ=255)

                                                   ggAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCAc                                                                              <  2:315101/140‑1 (MQ=255)

                                                        cgaagcTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACgttgt                                                                         >  2:178685/1‑140 (MQ=255)

                                                             cTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATg                                                                    >  2:220934/1‑140 (MQ=255)

                                                                 cggcACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGa                                                                >  1:870367/1‑140 (MQ=255)

                                                                    cACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCg                                                             <  2:502527/140‑1 (MQ=255)

                                                                         tGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTTTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGcaa                                                        >  2:470811/1‑140 (MQ=255)

                                                                                 ttGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCgg                                                >  1:47042/1‑140 (MQ=255)

                                                                                    cAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTgc                                             <  2:621566/140‑1 (MQ=255)

                                                                                          cTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGt                                       >  1:51997/1‑140 (MQ=255)

                                                                                               gCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAg                                  >  2:780358/1‑140 (MQ=255)

                                                                                                cGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGc                                 <  2:870367/140‑1 (MQ=255)

                                                                                                cGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGc                                 <  2:47042/140‑1 (MQ=255)

                                                                                                cGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGc                                 <  2:733053/140‑1 (MQ=255)

                                                                                                 gTATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCg                                >  2:31639/1‑140 (MQ=255)

                                                                                                  tATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGc                                 <  2:827624/138‑1 (MQ=255)

                                                                                                  tATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGc                                 >  1:827624/1‑138 (MQ=255)

                                                                                                  tATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGt                               >  2:461981/1‑140 (MQ=255)

                                                                                                  tATTGAAGCAAATCAGCGACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGt                               >  2:814848/1‑140 (MQ=255)

                                                                                                                   gACAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAAc              <  1:826684/140‑1 (MQ=255)

                                                                                                                    aCAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAAcc             <  2:589892/140‑1 (MQ=255)

                                                                                                                     cAAAACTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACca            <  1:780358/140‑1 (MQ=255)

                                                                                                                          cTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATc       <  2:806142/140‑1 (MQ=255)

                                                                                                                          cTTCACCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATc       <  1:178685/140‑1 (MQ=255)

                                                                                                                              aCCCGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGAt   >  1:53693/1‑140 (MQ=255)

                                                                                                                               cccGATCTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGAtt  >  1:602071/1‑140 (MQ=255)

GCGGCAATGGAAAAGGTTGCACAGGCAAAATCACCGCATGTTGCTGCGTTGGGAAGCGAAGCTGGCGGCACTTTGTACGGTTTGCAGGTACTGGAGCGTATTGAAGCAAATCAGCGACAAAACTTCACCCGATTTGTGGTGTTGGCGCGTAAAGCCATTAACGTGTCTGATCAGGTTCCGGCGAAAACCACGTTGTTAATGGCGACCGGGCAACAAGCCGGTGCGCTGGTTGAAGCGTTGCTGGTACTGCGCAACCACAATCTGATT  >  NC_000913/2738450‑2738716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A