Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,888,020	T→C	K98R (AAG→AGG)	cysH ←	phosphoadenosine phosphosulfate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,888,020	0	T	C	100.0%	115.9 / NA	34	K98R (AAG→AGG)	cysH	phosphoadenosine phosphosulfate reductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/18); total (16/18)

GCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGATCACCGGAATATCCGGGCGAATTTGATTCACCAGATGCAGGCTCACCGCCGCCTGAATGCCAAAG  >  NC_000913/2887885‑2888154

gCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAg                                                                                                                                    >  2:661096/1‑140 (MQ=255)

gCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAg                                                                                                                                    >  1:371281/1‑140 (MQ=255)

gCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAg                                                                                                                                    >  1:699724/1‑140 (MQ=255)

gCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAg                                                                                                                                    >  2:140359/1‑140 (MQ=255)

  ccGGTTCATCGCTTCGAATTTGTTGATGTCCTTGTCCTTTTCAATGCCTTCAACGCCCCGCTCCCACAGTTTTCCGTCGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCCGAGtt                                                                                                                                  <  2:879767/140‑1 (MQ=255)

   cGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGttt                                                                                                                                 <  1:325171/140‑1 (MQ=255)

     gTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGt                                                                                                                               >  2:91782/1‑140 (MQ=255)

            ggTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTaa                                                                                                                        <  2:769783/140‑1 (MQ=255)

                     ttgttgATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAAt                                                                                                               >  1:681463/1‑140 (MQ=255)

                      tgctgATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATa                                                                                                              <  2:763560/137‑1 (MQ=255)

                         tgATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAg                                                                                                           <  1:91782/140‑1 (MQ=255)

                             gTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTaa                                                                                                                        >  2:744996/1‑123 (MQ=255)

                             gTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTaa                                                                                                                        <  1:744996/123‑1 (MQ=255)

                                    tACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTc                                                                                                <  1:500582/140‑1 (MQ=255)

                                     aCTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTcc                                                                                               <  1:661096/140‑1 (MQ=255)

                                      cTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCg                                                                                              <  2:699724/140‑1 (MQ=255)

                                         ttCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGa                                                                                           <  1:814749/140‑1 (MQ=255)

                                                ccTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTa                                                                                    <  1:140359/140‑1 (MQ=255)

                                                ccTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTa                                                                                    <  1:196724/140‑1 (MQ=255)

                                                   tCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAAcc                                                                                 >  1:8050/1‑140 (MQ=255)

                                                           cTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCTGGGAACAAGTAACCCGTATCgg                                                                         >  2:400252/1‑140 (MQ=255)

                                                             gCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGt                                                                              <  1:52920/133‑1 (MQ=255)

                                                             gCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGt                                                                              >  2:52920/1‑133 (MQ=255)

                                                               tCCCACAGTTTTCCGTAGCGTACTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAg                                                                     <  1:695329/140‑1 (MQ=255)

                                                                   acaGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCTGGGAACAAGTAACCCGTATCGGTGAGGATc                                                                 <  1:400252/140‑1 (MQ=255)

                                                                                 cGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGATCACCGGAATATCCgg                                                   >  2:706438/1‑140 (MQ=255)

                                                                                     cTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGATCACCGGAATATCCGGGCGa                                               >  2:190375/1‑140 (MQ=255)

                                                                                            ccAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGATCACCGGAATATCCGGGCGAATTTGAt                                        <  2:371281/140‑1 (MQ=255)

                                                                                                                     tACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGATCACCGGAATATCCGGGCGAATTTGATTCACCAGATGCAGGCTCACcgccgc               <  2:681463/140‑1 (MQ=255)

                                                                                                                     tACACTTTCAGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGATCACCGGAATATCCGGGCGAATTTGATTCACCAGATGCAGGCTCACcgccgc               >  2:209945/1‑140 (MQ=255)

                                                                                                                              aGGTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGATCACCGGAATATCCGGGCGAATTTGATTCACCAGATGCAGGCTCACCGCCGCCTGAATGcc      <  1:190375/140‑1 (MQ=255)

                                                                                                                               ggTTGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGATCACCGGAATATCCGGGCGAATTTGATTCACCAGATGCAGGCTCACCGCCGCCTGAATGCCa     >  2:275972/1‑140 (MQ=255)

                                                                                                                                 ttGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGATCACCGGAATATCCGGGCGAATTTGATTCACCAGATGCAGGCTCACCCCCGCCTGAATGCCaaa   >  2:714387/1‑140 (MQ=255)

                                                                                                                                  tGAGCCTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGATCACCGGAATATCCGGGCGAATTTGATTCACCAGATGCAGGCTCACCGCCGCCTGAATGCCAAAg  >  2:394106/1‑140 (MQ=255)

GCCCGGTTCATCGGTTCGACTTTGTTGATGTCATTGTACTTTTCAATGCCTTCAACGCCCTGCTCCCACAGTTTTCCGTAGCGTGCTTCCTGCCAGGCTGCGCTTTCGGTAGCACGGTACACTTTCAGGTTGAGCTTGAGTTTGTCCGTTAACTCGTCAATAAAGCGGTAGGTTTCCGGGAACAAGTAACCCGTATCGGTGAGGATCACCGGAATATCCGGGCGAATTTGATTCACCAGATGCAGGCTCACCGCCGCCTGAATGCCAAAG  >  NC_000913/2887885‑2888154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A