Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,890,711	T→C	E396E (GAA→GAG)	cysJ ←	sulfite reductase, flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,890,711	0	T	C	100.0%	156.7 / NA	45	E396E (GAA→GAG)	cysJ	sulfite reductase, flavoprotein subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/21); total (24/21)

TATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGGGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGC  >  NC_000913/2890575‑2890843

tATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTcc                                                                                                                                   >  1:257422/1‑140 (MQ=255)

    ttCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCt                                                                                                                               >  1:819983/1‑140 (MQ=255)

       gATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCg                                                                                                                            >  2:391388/1‑140 (MQ=255)

        aTAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCga                                                                                                                           >  2:317402/1‑140 (MQ=255)

             tACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGaggagg                                                                                                                      <  1:272883/140‑1 (MQ=255)

             tACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGaggagg                                                                                                                      >  2:272883/1‑140 (MQ=255)

              aCGCGGACTTCGCNCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGc                                                                                                                     >  1:808077/1‑140 (MQ=255)

              aCGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGc                                                                                                                     >  1:4999/1‑140 (MQ=255)

                gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGa                                                                                                                   <  1:785411/140‑1 (MQ=255)

                gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGa                                                                                                                   <  1:875223/140‑1 (MQ=255)

                gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGa                                                                                                                   <  1:654260/140‑1 (MQ=255)

                gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGa                                                                                                                   <  2:257422/140‑1 (MQ=255)

                gcgGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGa                                                                                                                   <  2:81846/140‑1 (MQ=255)

                  ggACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATg                                                                                                                 <  1:317402/140‑1 (MQ=255)

                             tcttctTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGAcg                                                                                                      >  1:278556/1‑140 (MQ=255)

                                         cgGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCTACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGAcg                                                                                                      <  2:613402/128‑1 (MQ=255)

                                         cgGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGAcg                                                                                                      >  1:613402/1‑128 (MQ=255)

                                         cgGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCa                                                                                              <  2:800362/136‑1 (MQ=255)

                                         cgGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCa                                                                                              >  1:800362/1‑136 (MQ=255)

                                              aGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCgca                                                                                     <  1:475133/140‑1 (MQ=255)

                                              aGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCgca                                                                                     <  1:391388/140‑1 (MQ=255)

                                                            aGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAgc                                                                       >  2:823530/1‑140 (MQ=255)

                                                                accaccGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCtt                                                                   <  2:4999/140‑1 (MQ=255)

                                                                  caccGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCg                                                                 >  2:352513/1‑140 (MQ=255)

                                                                          cGGGCGCGGCCTTCCACGTCGTAACGCTCCACTCCAACGGTGGCGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAg                                                         <  2:819983/140‑1 (MQ=255)

                                                                          cGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAg                                                         >  2:54365/1‑140 (MQ=255)

                                                                            ggCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCt                                                       >  1:216013/1‑140 (MQ=255)

                                                                            ggCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCt                                                       >  2:77019/1‑140 (MQ=255)

                                                                                  gCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCg                                                                 <  1:682466/124‑1 (MQ=255)

                                                                                  gCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCg                                                                 >  2:682466/1‑124 (MQ=255)

                                                                                    cTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCggg                                               <  2:216013/140‑1 (MQ=255)

                                                                                             cgtAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCggg                                               >  2:6654/1‑131 (MQ=255)

                                                                                             cgtAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCggg                                               <  1:6654/131‑1 (MQ=255)

                                                                                             cgtAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGGGGAGAAACGc                                      <  1:77019/140‑1 (MQ=255)

                                                                                               tAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGGGGAGAAACGCAc                                    <  1:54365/140‑1 (MQ=255)

                                                                                                aaCGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGGGGAGAAACGCAcc                                   <  1:823530/140‑1 (MQ=255)

                                                                                                 aCGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGGGGACAAACGCACCa                                  <  1:193314/140‑1 (MQ=255)

                                                                                                       cacaccaACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACTCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGGGGAGAAACGCACCATGTcaa                            >  2:471364/1‑140 (MQ=255)

                                                                                                       cacaccaACGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGGGGAGAAACGCACCATGTcaa                            <  1:352513/140‑1 (MQ=255)

                                                                                                             aaCGGTGACGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGGGGAGAAACGCACCATGTCAACAATCg                      >  1:112085/1‑140 (MQ=255)

                                                                                                                    aCGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGGGGAGAAACGCACCATGTCAACAATCGGcgtcgt               >  1:494459/1‑140 (MQ=255)

                                                                                                                    aCGTGTACTTCGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGGGGAGAAACGCACCATGTCAACAATCGGcgtcgt               >  1:68028/1‑140 (MQ=255)

                                                                                                                              cGTTCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGGGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTaa     >  1:840823/1‑140 (MQ=255)

                                                                                                                                ttCTCGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGGGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATg   >  1:547033/1‑140 (MQ=255)

                                                                                                                                 tctcGACCTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGGGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGc  >  1:112637/1‑140 (MQ=255)

TATGTTCGATAAATACGCGGACTTCGCCCTCTTCTTCCACGCGGTCAGCGAGGAAGCTGGAGGCACCACCGGCACGGGCGCGGCCTTCCACGTCGTAACGCACCACACCAACGGTGACGTGTACTTCGTTCTCGACTTCCGCCTGCGAGGAGGCGATGGAATACAGACGCGGCGTCAGCGGGCGCAGCAGATTAATTAGCGCTTCGGCATCAAGCTGTGCCGGGGAGAAACGCACCATGTCAACAATCGGCGTCGTCGCGGCGTAATGC  >  NC_000913/2890575‑2890843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A