Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,897,037	A→G	G278G (GGT→GGC)	ygcS ←	putative transporter YgcS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,897,037	0	A	G	100.0%	139.7 / NA	40	G278G (GGT→GGC)	ygcS	putative transporter YgcS
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (22/18); total (22/18)

TTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGACCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAAAGAAGACGCTGTTAAACGCCGTGCGCCGCCAGTAACGCGAAGAGAACAAGGTTTTGAT  >  NC_000913/2896904‑2897162

ttGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATa                                                                                                                         >  1:556027/1‑140 (MQ=255)

ttGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATa                                                                                                                         >  1:706821/1‑140 (MQ=255)

 tGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAg                                                                                                                        >  1:570051/1‑140 (MQ=255)

  gccgccAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCa                                                                                                                            >  2:839306/1‑135 (MQ=255)

  gccgccAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCa                                                                                                                            <  1:839306/135‑1 (MQ=255)

  gccgccAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGt                                                                                                                       >  2:732469/1‑140 (MQ=255)

  gccgccAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGt                                                                                                                       <  1:562142/140‑1 (MQ=255)

               aaaaaCTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTggg                                                                                                                  <  2:722846/132‑1 (MQ=255)

               aaaaaCTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTggg                                                                                                                  >  1:722846/1‑132 (MQ=255)

                 aaaCTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTgg                                                                                                        <  1:661323/140‑1 (MQ=255)

                    cTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAg                                                                                                     >  1:773845/1‑140 (MQ=255)

                     ttCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGc                                                                                                    <  2:556027/140‑1 (MQ=255)

                     ttCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGc                                                                                                    <  2:570051/140‑1 (MQ=255)

                      tCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGcc                                                                                                   <  2:859538/140‑1 (MQ=255)

                      tCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGcc                                                                                                   <  1:713150/140‑1 (MQ=255)

                        ccAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAg                                                                                                 <  2:536700/140‑1 (MQ=255)

                            cAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGtat                                                                                             <  1:871268/140‑1 (MQ=255)

                                      cGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGcc                                                                                                   >  1:490236/1‑124 (MQ=255)

                                      cGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGcc                                                                                                   <  2:490236/124‑1 (MQ=255)

                                         tgtgCCAGCAGGGGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCa                                                                                >  2:789748/1‑140 (MQ=255)

                                                      gCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAgg                                                                   <  2:224615/140‑1 (MQ=255)

                                                       cGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGTTAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGc                                                                  >  2:561927/1‑140 (MQ=255)

                                                             aaCTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACaa                                                            >  1:642329/1‑140 (MQ=255)

                                                               cTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAaag                                                          >  2:160750/1‑140 (MQ=255)

                                                                  aTCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAaagaag                                                       >  2:798755/1‑140 (MQ=255)

                                                                   tCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAAagaaga                                                      <  1:160750/140‑1 (MQ=255)

                                                                     ccAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAAAGAAGACg                                                    <  1:732469/140‑1 (MQ=255)

                                                                            cgcgcCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAAAGAAGACGCTGTTaa                                             >  1:551856/1‑140 (MQ=255)

                                                                              cgcCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAAAGAAGACGCTGTTAAAc                                           >  2:827291/1‑140 (MQ=255)

                                                                                               gCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAAAGAAGACGCTGTTAAACGCCGTGCGCCGCCAGTa                          >  1:176542/1‑140 (MQ=255)

                                                                                               gCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAAAGAAGACGCTGTTAAACGCCGTGCGCCGCCAGTa                          >  2:422265/1‑140 (MQ=255)

                                                                                               acATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAAAGAAGACGCTGTTAAACGCCGTGCGCCGCCAGTa                          >  1:202351/2‑140 (MQ=255)

                                                                                                cATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAAAGAAGACGCTGTTAAACGCCGTGCGCCGCCAGTaa                         >  2:299976/1‑140 (MQ=255)

                                                                                                 aTTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAAAGAAGACGCTGTTAAACGCCGTGCGCCGCCAGTAAc                        <  2:393767/140‑1 (MQ=255)

                                                                                                    aaGCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAAAGAAGACGCTGTTAAACGCCGTGCGCCGCCAGTAAcgcg                     <  2:176542/140‑1 (MQ=255)

                                                                                                      gCATCAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAAAGAAGACGCTGTTAAACGCCGTGCGCCGCCAGTAACGCGaa                   <  1:789748/140‑1 (MQ=255)

                                                                                                          cAGGCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAAAGAAGACGCTGTTAAACGCCGTGCGCCGCCAGTAACGCGAAgaga               >  1:221433/1‑140 (MQ=255)

                                                                                                            ggCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAAAGAAGACGCTGTTAAACGCCGTGCGCCGCCAGTAACGCGAAGAGAAc             >  2:636212/1‑140 (MQ=255)

                                                                                                             gCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAAAGAAGACGCTGTTAAACGCCGTGCGCCGCCAGTAACGCGAAGAGAACa            >  1:777110/1‑140 (MQ=255)

                                                                                                             gCTGGCAGTCAGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAAAGAAGACGCTGTTAAACGCCGTGCGCCGCCAGTAACGCGAAGAGAACa            >  2:180668/1‑140 (MQ=255)

                                                                                                                       aGCGCATCTTCCAGGCCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAAAGAAGACGCTGTTAAACGCCGTGCGCCGCCAGTAACGCGAAGAGAACAAGGTTTTGAt  <  2:296085/140‑1 (MQ=255)

TTGCCGCCAGCAGCAAAAAACTTCCCAGCAAAAATTTGCGATGTGCCAGCAGGTGCGTCAGAACTAATCCCAGCAGCGCGCCCACAATTAACAACGCATTAAGCATCAGGCTGGCAGTCAGCGCATCTTCCAGACCAATAGTCTGGGCGATAGTTGGCAGCCAGGTATAAATCACAAACCATGGGATTACGAGGCAGACAAAGAAGACGCTGTTAAACGCCGTGCGCCGCCAGTAACGCGAAGAGAACAAGGTTTTGAT  >  NC_000913/2896904‑2897162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A