Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,898,921	T→C	T166T (ACA→ACG)	ygcU ←	putative FAD‑containing dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,898,921	0	T	C	100.0%	184.6 / NA	52	T166T (ACA→ACG)	ygcU	putative FAD‑containing dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/28); total (24/28)

GCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACAC  >  NC_000913/2898784‑2899051

gCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAgcgt                                                                                                                                  >  1:341123/1‑140 (MQ=255)

 cACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGcgtg                                                                                                                                 >  1:863796/1‑140 (MQ=255)

 cACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGcgtg                                                                                                                                 >  1:845968/1‑140 (MQ=255)

      tCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGaa                                                                                                                            <  2:124157/140‑1 (MQ=255)

      tCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGaa                                                                                                                            <  2:689516/140‑1 (MQ=255)

      tCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGaa                                                                                                                            <  1:672953/140‑1 (MQ=255)

       cGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAAc                                                                                                                           >  2:191584/1‑140 (MQ=255)

        gTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACt                                                                                                                          <  1:467482/140‑1 (MQ=255)

             cGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCg                                                                                                                     <  1:881268/140‑1 (MQ=255)

              gatgatgatGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGa                                                                                                                    >  2:717933/1‑140 (MQ=255)

                        tgACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGt                                                                                                          <  1:740972/140‑1 (MQ=255)

                        tgACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGt                                                                                                          <  1:635198/140‑1 (MQ=255)

                          aCGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGccc                                                                                                                      <  1:116395/126‑1 (MQ=255)

                          aCGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGccc                                                                                                                      >  2:116395/1‑126 (MQ=255)

                          aCGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTg                                                                                                        >  2:763974/1‑140 (MQ=255)

                             aaTGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTa                                                                                                     <  2:537602/140‑1 (MQ=255)

                              aTGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTAc                                                                                                    >  2:860830/1‑140 (MQ=255)

                               tGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTAcc                                                                                                   <  2:557009/140‑1 (MQ=255)

                               tGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTAcc                                                                                                   >  2:4680/1‑140 (MQ=255)

                                 tCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAg                                                                                                 >  1:717809/1‑140 (MQ=255)

                                 tCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAg                                                                                                 >  2:84760/1‑140 (MQ=255)

                                  ccGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAgg                                                                                                >  1:355355/1‑140 (MQ=255)

                                    gggCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGt                                                                                                          >  2:816297/1‑128 (MQ=255)

                                    gggCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGt                                                                                                          <  1:816297/128‑1 (MQ=255)

                                      gCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGgccgc                                                                                            <  2:717809/140‑1 (MQ=255)

                                          gCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCAt                                                                                        >  1:278066/1‑140 (MQ=255)

                                            cgcgggGCGTGACACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTACGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGCTTGCTACCAGGCGGCCCATCt                                                                                      <  2:628782/140‑1 (MQ=255)

                                                  gcgTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCa                                                                                <  2:113857/140‑1 (MQ=255)

                                                           gTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTg                                                                       <  1:84760/140‑1 (MQ=255)

                                                                     cGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGgaga                                                             <  2:845968/140‑1 (MQ=255)

                                                                       tgtgACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCg                                                                 >  2:579888/1‑134 (MQ=255)

                                                                       tgtgACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCg                                                                 <  1:579888/134‑1 (MQ=255)

                                                                       tgtgACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAAt                                                           >  2:565919/1‑140 (MQ=255)

                                                                            cGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGcccc                                                      <  2:341123/140‑1 (MQ=255)

                                                                             ggTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTTACTGCGGAGAATGCCCCg                                                     <  1:751153/140‑1 (MQ=255)

                                                                             ggTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCg                                                     <  2:656088/140‑1 (MQ=255)

                                                                                         cAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCtt                                         >  2:409656/1‑140 (MQ=255)

                                                                                           aTACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCtttt                                       >  2:716323/1‑140 (MQ=255)

                                                                                                  ttCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCa                                <  2:603096/140‑1 (MQ=255)

                                                                                                      aGACCAACGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAAcgc                            >  1:697090/1‑140 (MQ=255)

                                                                                                             cGACCATATCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTcc                     >  2:67533/1‑140 (MQ=255)

                                                                                                                     tCTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTc             >  1:28557/1‑140 (MQ=255)

                                                                                                                      cTTCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTcc            <  1:214177/140‑1 (MQ=255)

                                                                                                                        tCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAg          <  1:177594/140‑1 (MQ=255)

                                                                                                                        tCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAg          <  2:355355/140‑1 (MQ=255)

                                                                                                                        tCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAg          <  1:4680/140‑1 (MQ=255)

                                                                                                                        tCGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAg          <  1:409656/140‑1 (MQ=255)

                                                                                                                         cGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAg                   >  2:656580/1‑130 (MQ=255)

                                                                                                                         cGATTGCGCCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAg                   <  1:656580/130‑1 (MQ=255)

                                                                                                                              gcgcCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAac    >  1:532404/1‑140 (MQ=255)

                                                                                                                               cgcCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAaca   >  1:743250/1‑140 (MQ=255)

                                                                                                                               cgcCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAaca   <  1:565919/140‑1 (MQ=255)

                                                                                                                               cgcCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAaca   >  1:409526/1‑140 (MQ=255)

                                                                                                                               cgcCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAaca   >  1:303699/1‑140 (MQ=255)

                                                                                                                               cgcCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAaca   <  2:863796/140‑1 (MQ=255)

                                                                                                                               cgcCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGCAacc   >  1:33171/1‑139 (MQ=255)

                                                                                                                                gcCGTAGAGCGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAacac  <  2:28557/140‑1 (MQ=255)

GCACCTTCGTTGCCGATGATGATGTGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACAC  >  NC_000913/2898784‑2899051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A