Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,083,475	T→C	H108R (CAC→CGC)	speB ←	agmatinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,083,475	0	T	C	100.0%	194.2 / NA	55	H108R (CAC→CGC)	speB	agmatinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/33); total (22/33)

TCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGTGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGT  >  NC_000913/3083340‑3083608

tCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGgcgcgcgc                                                                                                                                   <  2:255244/140‑1 (MQ=255)

 cGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGcc                                                                                                                                  <  2:501114/140‑1 (MQ=255)

 cGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGcc                                                                                                                                  <  2:22932/140‑1 (MQ=255)

     gtgGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCa                                                                                                                              <  1:603508/140‑1 (MQ=255)

      tgGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTg                                                                                                                                <  1:694312/137‑1 (MQ=255)

      tgGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTg                                                                                                                                >  2:694312/1‑137 (MQ=255)

       gggCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGc                                                                                                                            >  2:710443/1‑140 (MQ=255)

       gggCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGc                                                                                                                            >  1:855060/1‑140 (MQ=255)

       gggCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGc                                                                                                                            >  2:446519/1‑140 (MQ=255)

       gggCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGc                                                                                                                            >  2:651170/1‑140 (MQ=255)

        ggCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCt                                                                                                                           >  2:441605/1‑140 (MQ=255)

               aaGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCt                                                                                                                    <  2:703100/140‑1 (MQ=255)

               aaGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCt                                                                                                                    <  1:828243/140‑1 (MQ=255)

                  tgtACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCAt                                                                                                                 <  1:705945/140‑1 (MQ=255)

                    tACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCAtct                                                                                                               >  2:302918/1‑140 (MQ=255)

                      ccAGCGCCGTTTTGCCGAAATGCTTCGCATGAGCGCGCAGCGGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATGCGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCGTGCAGCTTTTCGCTCATCTCa                                                                                                             <  1:419486/140‑1 (MQ=255)

                              aTTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATc                                                                                                     >  2:232856/1‑140 (MQ=255)

                                 ttGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGcc                                                                                                  >  2:399119/1‑140 (MQ=255)

                                  tGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCa                                                                                                 <  2:855060/140‑1 (MQ=255)

                                  tGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCa                                                                                                 <  1:28746/140‑1 (MQ=255)

                                  tGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCa                                                                                                 <  1:384974/140‑1 (MQ=255)

                                     cGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAg                                                                                              <  1:651170/140‑1 (MQ=255)

                                     cGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAg                                                                                              <  1:719189/140‑1 (MQ=255)

                                       aaaTGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGc                                                                                            <  1:710443/140‑1 (MQ=255)

                                       aaaTGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGc                                                                                            <  1:750925/140‑1 (MQ=255)

                                         aTGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCat                                                                                          >  1:239710/1‑140 (MQ=255)

                                              tCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAg                                                                                              <  1:315744/131‑1 (MQ=255)

                                              tCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAg                                                                                              >  2:315744/1‑131 (MQ=255)

                                                       cACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGTCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATcgccg                                                                            <  2:277954/140‑1 (MQ=255)

                                                       cACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATcgccg                                                                            <  1:302918/140‑1 (MQ=255)

                                                       cACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATcgccg                                                                            <  1:441605/140‑1 (MQ=255)

                                                         cGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATcgccg                                                                            >  1:619187/1‑138 (MQ=255)

                                                         cGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATcgccg                                                                            <  2:619187/138‑1 (MQ=255)

                                                           cagcggCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGt                                                                        <  1:446519/140‑1 (MQ=255)

                                                              cagcggcagcgTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCa                                                                     <  1:89245/140‑1 (MQ=255)

                                                                         tAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGa                                                          <  1:450996/140‑1 (MQ=255)

                                                                               aGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCa                                                    <  2:771146/140‑1 (MQ=255)

                                                                                 tGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACg                                                  >  2:708227/1‑140 (MQ=255)

                                                                                               agagagCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTcc                                    <  1:726101/140‑1 (MQ=255)

                                                                                                  gagCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACg                                 >  1:552826/1‑140 (MQ=255)

                                                                                                   agCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACgg                                >  1:266363/1‑140 (MQ=255)

                                                                                                       tACGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAg                            <  1:232856/140‑1 (MQ=255)

                                                                                                         cGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCg                          >  2:193186/1‑140 (MQ=255)

                                                                                                         cGCTTACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCg                          <  1:399119/140‑1 (MQ=255)

                                                                                                            ttACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGtt                       <  1:193186/140‑1 (MQ=255)

                                                                                                            ttACCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGtt                       <  2:266363/140‑1 (MQ=255)

                                                                                                              aCCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTtgt                     <  1:760034/140‑1 (MQ=255)

                                                                                                              aCCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTtgt                     >  1:756999/1‑140 (MQ=255)

                                                                                                              aCCGGCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTtgt                     >  1:61437/1‑140 (MQ=255)

                                                                                                                  gCAGCCAGCAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTc                 >  2:840985/1‑140 (MQ=255)

                                                                                                                    agccagcAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTccc               >  2:792273/1‑140 (MQ=255)

                                                                                                                      ccagcAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTcc                >  2:473256/1‑137 (MQ=255)

                                                                                                                      ccagcAGCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTcc                <  1:473256/137‑1 (MQ=255)

                                                                                                                       cagcagCTTCTCGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAgg            <  1:840985/140‑1 (MQ=255)

                                                                                                                                 tcGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATtcgt  <  1:708227/140‑1 (MQ=255)

                                                                                                                                 tcGGCGCGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATtcgt  >  2:358284/1‑140 (MQ=255)

TCGGTGTGGGCGTCAAAGTGTACCAGCGCCATTTTGCCGAAATGCTTCGCATGAGCACGCAGCAGCGGCAGCGTAACAAAGTGGTCACCACCGAAAGAGAGCATACGCTTACCGGCAGCCAGCAGCTTCTCGGCGTGCGCCTGCAGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGT  >  NC_000913/3083340‑3083608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A