Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,195,534	T→C	D407G (GAC→GGC)	rfaE ←	fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,195,534	0	T	C	100.0%	180.8 / NA	51	D407G (GAC→GGC)	rfaE	fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/26); total (25/26)

CGCCACCGTTGGCCCAGACTTCTTTACTCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGCATCGCTGTTGACGGCAACAATCAAGCGGTCACCCAGCTTGC  >  NC_000913/3195400‑3195663

cGCCACCGTTGGCCCAGACTTCTTTACTCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGAcc                                                                                                                              >  2:862469/1‑140 (MQ=255)

        ttGGCCCAGACTTCTTTACTCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGAc                                                                                                                               >  1:32594/1‑131 (MQ=255)

        ttGGCCCAGACTTCTTTACTCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGAc                                                                                                                               <  2:32594/131‑1 (MQ=255)

         tGGCCCAGACTTCTTTACTCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGt                                                                                                                     <  2:466066/140‑1 (MQ=255)

          ggCCCAGACTTCTTTACTCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTg                                                                                                                    >  1:580257/1‑140 (MQ=255)

            cccAGACTTCTTTACTCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTgcg                                                                                                                  >  2:327380/1‑140 (MQ=255)

            cccAGACTTCTTTACTCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTgcg                                                                                                                  <  1:54489/140‑1 (MQ=255)

               aGACTTCTTTACTCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGccc                                                                                                               <  2:172921/140‑1 (MQ=255)

                 aCTTCTTTACTCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAg                                                                                                             <  2:580647/140‑1 (MQ=255)

                   ttcttTACTCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGca                                                                                                           <  2:147127/140‑1 (MQ=255)

                   ttcttTACTCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGca                                                                                                           <  2:353731/140‑1 (MQ=255)

                   ttcttTACTCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGca                                                                                                           <  1:872074/140‑1 (MQ=255)

                   ttcttTACTCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGca                                                                                                           <  1:862469/140‑1 (MQ=255)

                     cttTACTCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGcaca                                                                                                         >  1:747277/1‑140 (MQ=255)

                     cttTACTCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGcaca                                                                                                         >  1:238077/1‑140 (MQ=255)

                      tttACTCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACaa                                                                                                        >  2:234802/1‑140 (MQ=255)

                           tCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATa                                                                                                   <  2:70744/140‑1 (MQ=255)

                                                tataGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTAcc                                                                              >  1:46955/1‑140 (MQ=255)

                                                  taGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTa                                                                                >  1:552751/1‑136 (MQ=255)

                                                  taGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTa                                                                                <  2:552751/136‑1 (MQ=255)

                                                       gccgccTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACcgggcggg                                                                       >  1:371527/1‑140 (MQ=255)

                                                       gccgccTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACcgggcggg                                                                       >  2:164810/1‑140 (MQ=255)

                                                        ccgccTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGa                                                                      >  2:252078/1‑140 (MQ=255)

                                                        ccgccTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGa                                                                      >  2:741075/1‑140 (MQ=255)

                                                            cTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATcc                                                                  <  1:327380/140‑1 (MQ=255)

                                                                 aCCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCttt                                                             <  1:252078/140‑1 (MQ=255)

                                                                    agcagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAg                                                          <  1:763343/140‑1 (MQ=255)

                                                                      cagATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGcc                                                        >  2:494853/1‑140 (MQ=255)

                                                                        gATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGt                                                      <  2:751300/140‑1 (MQ=255)

                                                                              gCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTtggtg                                                <  1:164810/140‑1 (MQ=255)

                                                                                  gATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAgg                                            <  1:741075/140‑1 (MQ=255)

                                                                                  gATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAgg                                            <  2:238077/140‑1 (MQ=255)

                                                                                   aTCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAgg                                            >  2:769082/1‑139 (MQ=255)

                                                                                   aTCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAgg                                            <  1:769082/139‑1 (MQ=255)

                                                                                   aTCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGc                                           >  1:91292/1‑140 (MQ=255)

                                                                                    tCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGCa                                          <  2:580257/140‑1 (MQ=255)

                                                                                             tCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGCATCGCTGTTg                                 >  2:352388/1‑140 (MQ=255)

                                                                                                 gcgcTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGCATCGCTGTTGACgg                             <  1:398631/140‑1 (MQ=255)

                                                                                                    cTGCGGCGTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGCATCGCTGTTGACGGcaa                          <  1:494853/140‑1 (MQ=255)

                                                                                                      gcggcgTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGCATCGCTGTTGACGGcaaca                        <  2:46955/140‑1 (MQ=255)

                                                                                                       cggcgTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGCATCGCTGTTGa                                <  2:145763/131‑1 (MQ=255)

                                                                                                       cggcgTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGCATCGCTGTTGa                                >  1:145763/1‑131 (MQ=255)

                                                                                                       cggcgTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGCATCGCTGTTGACGGcaacaa                       >  1:546375/1‑140 (MQ=255)

                                                                                                       cggcgTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGCATCGCTGTTGACGGcaacaa                       >  2:600985/1‑140 (MQ=255)

                                                                                                       cggcgTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGCATCGCTGTTGACGGcaacaa                       >  2:162279/1‑140 (MQ=255)

                                                                                                          cgTGTCCTCTTCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGCATCGCTGTTGACGGCAACAATCa                    <  2:284690/140‑1 (MQ=255)

                                                                                                                   ttCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGCATCGCTGTTGACGGCAACAATCAAGCg                >  1:220899/1‑135 (MQ=255)

                                                                                                                   ttCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGCATCGCTGTTGACGGCAACAATCAAGCg                <  2:220899/135‑1 (MQ=255)

                                                                                                                   ttCAAACGACACTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGCATCGCTGTTGACGGCAACAATCAAGCGGTCAc           >  1:210888/1‑140 (MQ=255)

                                                                                                                           acacTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGCATCGCTGTTGACGGCAACAATCAAGCGGTCACCCAGCTTg   >  2:268747/1‑140 (MQ=255)

                                                                                                                            cacTACCCAGCCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGCATCGCTGTTGACGGCAACAATCAAGCGGTCACCCAGCTTGc  >  2:121910/1‑140 (MQ=255)

CGCCACCGTTGGCCCAGACTTCTTTACTCCCGGCAATCTCTTCTGGTTTATAGTCGCCGCCTTTCACCAGCAGATCTGGCAAGATCCCGGCGATCAAGCGCTGCGGCGTGTCCTCTTCAAACGACACTACCCAGTCGACCGCTTCCAGTGCGCCCAGCACAATCATACGCTGTTCGAGTGGGTTTACCGGGCGGGAATCCCCTTTCAGCCGTTTGGTGGAGGCATCGCTGTTGACGGCAACAATCAAGCGGTCACCCAGCTTGC  >  NC_000913/3195400‑3195663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A