Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,264,839	T→A	Y60F (TAC→TTC)	tdcC ←	threonine/serine:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,264,839	0	T	A	100.0%	131.4 / NA	38	Y60F (TAC→TTC)	tdcC	threonine/serine:H(+) symporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (20/18); total (20/18)

AAGTACAGGAACGTGATAACCACGCCGCCAGTTTTACCAAAATGCTCTTCCACCGTTTCCGTAATGTTGCCGGAAGGGTTAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGTATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACG  >  NC_000913/3264704‑3264974

aagTACAGGAACGTGATAACCACGCCGCCAGTTTTACCAAAATGCTCTTCCACCGTTTCCGTAATGTTGCCGGAAGGGTTAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGc                                                                                                                                     <  2:173107/140‑1 (MQ=255)

       ggAACGTGATAACCACGCCGCCAGTTTTACCAAAATGCTCTTCCACCGTTTCCGTAATGTTGCCGGAAGGGTTAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATAcc                                                                                                                              <  1:275310/140‑1 (MQ=255)

          aCGTGATAACCACGCCGCCAGTTTTACCAAAATGCTCTTCCACCGTTTCCGTAATGTTGCCGGAAGGGTTAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAAc                                                                                                                           >  2:726590/1‑140 (MQ=255)

          aCGTGATAACCACGCCGCCAGTTTTACCAAAATGCTCTTCCACCGTTTCCGTAATGTTGCCGGAAGGGTTAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAAc                                                                                                                           >  1:81802/1‑140 (MQ=255)

                      cgccgcCAGTTTTACCAAAATGCTCTTCCACCGTTTCCGTAATGTTGCCGGAAGGGTTAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATc                                                                                                               >  1:848539/1‑140 (MQ=255)

                                      aaaaTGCTCTTCCACCGTTTCCGTAATGTTGCCGGAAGGGTTAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCaa                                                                                               >  2:621856/1‑140 (MQ=255)

                                                  cACCGTTTCCGTAATGTTGCCGGAAGGGTTAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGa                                                                                   <  2:25346/140‑1 (MQ=255)

                                                  cACCGTTTCCGTAATGTTGCCGGAAGGGTTAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGa                                                                                   <  1:406890/140‑1 (MQ=255)

                                                    ccGTTTCCGTAATGTTGCCGGAAGGGTTAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATa                                                                                 <  2:170338/140‑1 (MQ=255)

                                                    ccGTTTCCGTAATGTTGCCGGAAGGGTTAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATa                                                                                 <  2:125759/140‑1 (MQ=255)

                                                           cGTAATGTTGCCGGAAGGGTTAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGgaaga                                                                          >  1:858950/1‑140 (MQ=255)

                                                                 gTTGCCGGAAGGGGTAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCa                                                                    >  2:831455/1‑140 (MQ=255)

                                                                    gCCGGAAGGGTTAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCAccc                                                                 >  1:201623/1‑140 (MQ=255)

                                                                    gCCGGAAGGGTTAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCAccc                                                                 >  1:377191/1‑140 (MQ=255)

                                                                      cGGAAGGGTTAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCg                                                               <  2:466605/140‑1 (MQ=255)

                                                                         aaGGGTTAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGgcg                                                            >  1:128778/1‑140 (MQ=255)

                                                                              ttAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGAt                                                       >  1:200040/1‑140 (MQ=255)

                                                                                agagCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTg                                                     <  1:591856/140‑1 (MQ=255)

                                                                                agagCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTg                                                     >  1:780025/1‑140 (MQ=255)

                                                                                agagCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTg                                                     <  1:732651/140‑1 (MQ=255)

                                                                                  agCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGcc                                                   <  2:377191/140‑1 (MQ=255)

                                                                                      aGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTAc                                               >  1:425565/1‑140 (MQ=255)

                                                                                       gAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTAcc                                              >  1:241927/1‑140 (MQ=255)

                                                                                       gAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGAGTGCCGTAcc                                              >  2:129593/1‑140 (MQ=255)

                                                                                              acaGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAg                                       >  1:590428/1‑140 (MQ=255)

                                                                                                   cgcgCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTaa                                  <  2:425565/140‑1 (MQ=255)

                                                                                                   cgcgCCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTaa                                  <  1:768124/140‑1 (MQ=255)

                                                                                                     cgcCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACg                                <  2:17027/140‑1 (MQ=255)

                                                                                                      gcCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTaa                                  <  1:63090/137‑1 (MQ=255)

                                                                                                      gcCAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTaa                                  >  2:63090/1‑137 (MQ=255)

                                                                                                       ccAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTc                              <  2:200040/140‑1 (MQ=255)

                                                                                                       ccAGCGCCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTc                              >  1:879275/1‑140 (MQ=255)

                                                                                                            gcCCGGTGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCAtgt                         >  1:384331/1‑140 (MQ=255)

                                                                                                                  tGGCAATAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATc                   <  2:241927/140‑1 (MQ=255)

                                                                                                                       aTAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGAtt              <  1:831455/140‑1 (MQ=255)

                                                                                                                        tAAAACGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGAttt             >  1:387007/1‑140 (MQ=255)

                                                                                                                           aaCGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCAtg                          <  2:71614/124‑1 (MQ=255)

                                                                                                                           aaCGCGATGGGGAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCAtg                          >  1:71614/1‑124 (MQ=255)

                                                                                                                                   ggggAATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACg  >  2:491415/1‑140 (MQ=255)

AAGTACAGGAACGTGATAACCACGCCGCCAGTTTTACCAAAATGCTCTTCCACCGTTTCCGTAATGTTGCCGGAAGGGTTAGAGCCAGAAAGACACAGACGCGCCAGCGCCCGGTGGCAATAAAACGCGATGGGGTATGCCAATACCAACATCAGAAGAATCGGGATCAGTCCGCCAAAACCTGCGCGGATAGGGAAGAACAGCACCCCGGCGCCGATTGCCGTACCAAACAAGCCTAACGTCCATGTGGTATCTGATTTACGCCAGGACG  >  NC_000913/3264704‑3264974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A