Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,265,564	A→T	D155E (GAT→GAA)	tdcB ←	catabolic threonine dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,265,564	0	A	T	100.0%	195.7 / NA	56	D155E (GAT→GAA)	tdcB	catabolic threonine dehydratase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (37/19); total (37/19)

ATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGA  >  NC_000913/3265437‑3265695

aTTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGt                                                                                                                         >  1:188156/1‑140 (MQ=255)

  ttAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTgg                                                                                                                       >  2:231954/1‑140 (MQ=255)

  ttAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTgg                                                                                                                       >  1:52539/1‑140 (MQ=255)

     aTTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGAt                                                                                                                    >  1:317498/1‑140 (MQ=255)

                    ccAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTc                                                                                                     >  2:420376/1‑140 (MQ=255)

                       gCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATaaaaa                                                                                                               >  2:713700/1‑127 (MQ=255)

                       gCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATaaaaa                                                                                                               <  1:713700/127‑1 (MQ=255)

                       gCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATATGGCCTTCCAt                                                                                                  >  1:331170/1‑140 (MQ=255)

                       gCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCa                                                                                                   >  2:447354/1‑139 (MQ=255)

                       gCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCa                                                                                                   <  1:447354/139‑1 (MQ=255)

                       gCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCAt                                                                                                  >  1:837679/1‑140 (MQ=255)

                                   ccaccaccaATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTc                                                                                      >  2:814349/1‑140 (MQ=255)

                                      ccaccaATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCt                                                                                   <  1:14235/140‑1 (MQ=255)

                                      ccaccaATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCt                                                                                   <  2:837679/140‑1 (MQ=255)

                                      ccaccaATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCt                                                                                   <  1:420376/140‑1 (MQ=255)

                                       caccaATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTc                                                                                  <  1:814349/140‑1 (MQ=255)

                                              tGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAg                                                                           >  2:362925/1‑140 (MQ=255)

                                                 cacaATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGa                                                                        >  2:191492/1‑140 (MQ=255)

                                                      tCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAgtg                                                                   >  2:640816/1‑140 (MQ=255)

                                                           ttATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGtt                                                              <  2:52539/140‑1 (MQ=255)

                                                           ttATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGtt                                                              <  2:317498/140‑1 (MQ=255)

                                                           ttATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGtt                                                              <  2:297558/140‑1 (MQ=255)

                                                              tCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGaa                                                           <  1:362925/140‑1 (MQ=255)

                                                                gACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGt                                                         >  1:591578/1‑140 (MQ=255)

                                                                   atcatAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTAt                                                      <  1:640816/140‑1 (MQ=255)

                                                                    tcatAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGtt                                                              >  1:337375/1‑131 (MQ=255)

                                                                    tcatAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGtt                                                              <  2:337375/131‑1 (MQ=255)

                                                                     catAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCa                                                    >  2:373776/1‑140 (MQ=255)

                                                                      atAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGtt                                                              >  2:282322/1‑129 (MQ=255)

                                                                      atAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGtt                                                              <  1:282322/129‑1 (MQ=255)

                                                                        agagATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGtt                                                              >  1:102950/1‑127 (MQ=255)

                                                                        agagATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGtt                                                              <  2:102950/127‑1 (MQ=255)

                                                                            aTCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCa                                             >  1:635798/1‑140 (MQ=255)

                                                                            aTCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAAGCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCAGTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCa                                             >  2:525007/1‑140 (MQ=255)

                                                                             tCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAg                                            >  2:423869/1‑140 (MQ=255)

                                                                               ttCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGaa                                          >  1:746607/1‑140 (MQ=255)

                                                                               ttCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGaa                                          >  1:679152/1‑140 (MQ=255)

                                                                                 ccATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACg                                        >  1:41137/1‑140 (MQ=255)

                                                                                   aTAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGAc                                      <  2:188156/140‑1 (MQ=255)

                                                                                    tAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACt                                     >  1:485224/1‑140 (MQ=255)

                                                                                        ttCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTg                                 <  1:525007/140‑1 (MQ=255)

                                                                                        ttCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTg                                 >  2:554866/1‑140 (MQ=255)

                                                                                         tCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTGCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGc                                >  2:703050/1‑140 (MQ=255)

                                                                                            aGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGa                             <  2:591578/140‑1 (MQ=255)

                                                                                                  aTCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTc                       >  2:82235/1‑140 (MQ=255)

                                                                                                   tCGTTCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCg                      >  1:72429/1‑140 (MQ=255)

                                                                                                      ttCCCTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCAc                   <  2:679152/140‑1 (MQ=255)

                                                                                                          cTGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTg               >  2:219282/1‑140 (MQ=255)

                                                                                                           tGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACg                  <  1:211126/136‑1 (MQ=255)

                                                                                                           tGGCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACg                  >  2:211126/1‑136 (MQ=255)

                                                                                                             gCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCg            >  2:744778/1‑140 (MQ=255)

                                                                                                             gCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCg            >  2:748973/1‑140 (MQ=255)

                                                                                                             gCCAGCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCg            >  1:670642/1‑140 (MQ=255)

                                                                                                                 gCAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTAc        >  2:467433/1‑140 (MQ=255)

                                                                                                                  cAATCACTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACt       >  1:289111/1‑140 (MQ=255)

                                                                                                                       aCTTTCGGTTCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGa  >  2:829715/1‑140 (MQ=255)

ATTTAATTGCCACCGCAATACCAGCAATTAAACCGCCACCACCAATTGGCACAATCACGTTATCGACATCATAGAGATCTTCCATAATTTCCAGACCAATCGTTCCCTGGCCAGCAATCACTTTCGGATCATCGTAAGGTGGGATAAAAATACGGCCTTCCATTTCGACAATTTCGCTCACTTTAGCGATAGTGTCGTTGAAGTTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGA  >  NC_000913/3265437‑3265695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A