Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,267,015	T→C	E17E (GAA→GAG)	tdcA ←	DNA‑binding transcriptional activator TdcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,267,015	0	T	C	100.0%	137.3 / NA	39	E17E (GAA→GAG)	tdcA	DNA‑binding transcriptional activator TdcA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/22); total (17/22)

ACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAATGAAATGAACAGGATATGTGCGACCACTCACAAATTA  >  NC_000913/3266884‑3267149

ccacCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGaaa                                                                                                                                <  1:696866/139‑1 (MQ=255)

acacCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGaaa                                                                                                                                >  2:756115/1‑140 (MQ=255)

  acCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTACCCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGa                                                                                                                              <  2:227176/140‑1 (MQ=255)

      gTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTAc                                                                                                                          >  1:84042/1‑140 (MQ=255)

      gTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTAc                                                                                                                          >  1:812829/1‑140 (MQ=255)

                        aaTTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCgg                                                                                                        <  2:632968/140‑1 (MQ=255)

                                  aaaaaTAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCAAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATa                                                                                              <  1:167630/139‑1 (MQ=255)

                                     aataatCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTCCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAggg                                                                                           >  1:752269/1‑138 (MQ=255)

                                           tttCAATTTCGTTAATGATTTTACTGGCGGCGGGTGGAGTTACCCATAATCTTTTTCCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATa                                                                                     <  2:84042/139‑1 (MQ=255)

                                               aaTATCTTTAATAATTTTACTGCGGGCGGGTTGAGTTACCCCTAATTCTTTTGCGGCGGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGa                                                                                 <  1:152134/140‑1 (MQ=255)

                                                 tatCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGAcc                                                                               >  2:406651/1‑140 (MQ=255)

                                                     gTTAATGTTTTTACGGCGGGCCGGTGGATTTAACCCTAATTCTTTTGCAGCGGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGCTTTCGGAAGAAGAATAGTGCTCATAACGACCTACg                                                                           <  1:709469/140‑1 (MQ=255)

                                                      ttAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACg                                                                                  >  2:169236/1‑132 (MQ=255)

                                                      ttAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACg                                                                                  <  1:169236/132‑1 (MQ=255)

                                                      ttAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGt                                                                          >  1:261971/1‑140 (MQ=255)

                                                           gtttttACTGACGGCGGGTTGATTTACCCCTAATTTTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAAtt                                                                     <  2:829780/138‑1 (MQ=255)

                                                           gATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAAtt                                                                     <  1:337984/140‑1 (MQ=255)

                                                              tttACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTAcc                                                                  >  2:835772/1‑140 (MQ=255)

                                                                 aCTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCa                                                               >  1:395350/1‑140 (MQ=255)

                                                                   tGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCAtt                                                             >  2:254599/1‑140 (MQ=255)

                                                                    gccGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTg                                                            <  2:144419/138‑1 (MQ=255)

                                                                     ccggcggGTTGAGTAACCCCTAATTCTTTTCCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGa                                                           <  2:261971/139‑1 (MQ=255)

                                                                     aCGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGa                                                           <  2:720667/140‑1 (MQ=255)

                                                                     aCGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGa                                                           >  2:400088/1‑140 (MQ=255)

                                                                     aCGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGa                                                           >  2:152883/1‑140 (MQ=255)

                                                                       ggCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACg                                                         >  2:320833/1‑140 (MQ=255)

                                                                            gttaagttaaCCCTAATTCTTTTGCAGCGGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATg                                                    <  1:572616/140‑1 (MQ=255)

                                                                             ttGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGa                                                   <  1:152883/140‑1 (MQ=255)

                                                                             ttGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGa                                                   <  1:400088/140‑1 (MQ=255)

                                                                                    aaCCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTAt                                            <  2:812829/140‑1 (MQ=255)

                                                                                        cTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCaaa                                        <  1:694636/140‑1 (MQ=255)

                                                                                                  tGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAatgaaatgaa                              <  2:683609/140‑1 (MQ=255)

                                                                                                  tGCAGCCGAGCCGATAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAatgaaatgaa                              <  1:406651/140‑1 (MQ=255)

                                                                                                  gacaccagagccgaTAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAatgaaatgaa                              <  2:163286/133‑1 (MQ=255)

                                                                                                         gagccgaTAGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAATGAAATGAACAGGata                       >  2:642027/1‑140 (MQ=255)

                                                                                                                 aGAACCACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAATGAAATGAACAGGATATGTGCGAc               >  1:58928/1‑140 (MQ=255)

                                                                                                                     ccACTTCTAATGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAATGAAATGAACAGGATATGTGCGACCACt           >  1:641764/1‑140 (MQ=255)

                                                                                                                             aaTGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAATGAAATGAACAGGATATGTGCGACCACTCACAAAtt   >  2:494325/1‑140 (MQ=255)

                                                                                                                              aTGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAATGAAATGAACAGGATATGTGCGACCACTCACAAATTa  <  2:692551/140‑1 (MQ=255)

                                                                                                                              aTGACCTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCAGGAAGTGTATCAAAATGAAATGAACAGGATATGTGCGACCACTCACAAATTa  <  2:627357/140‑1 (MQ=255)

ACACCGGTATTTTTCCGCACCACTAATTCCACACCAAAATAATCTTCAATATCGTTAATGATTTTACTGACGGCCGGTTGAGTTAACCCTAATTCTTTTGCAGCCGAGCCGATAGAACCACTTCTAATGACTTCCTGAAAGACTACCAGGTGCTGCGTTTTCGGAAGAAGAATAGTGCTCATAACGACCTACGTTAATTACCTCATTGACGGCATGAAGTGTATCAAAATGAAATGAACAGGATATGTGCGACCACTCACAAATTA  >  NC_000913/3266884‑3267149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A