Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,312,238	G→A	I180I (ATC→ATT)	truB ←	tRNA pseudouridine(55) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,312,238	0	G	A	100.0%	168.1 / NA	47	I180I (ATC→ATT)	truB	tRNA pseudouridine(55) synthase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (19/28); total (19/28)

CTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCAATGCCCTGACGCGCATATTCGTACAGTT  >  NC_000913/3312106‑3312374

cTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAg                                                                                                                                   <  1:126968/140‑1 (MQ=255)

 tCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAgg                                                                                                                                  >  2:34548/1‑139 (MQ=255)

 tCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGt                                                                                                                                  <  2:28475/140‑1 (MQ=255)

 tCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGt                                                                                                                                  <  2:483747/140‑1 (MQ=255)

   aacaaGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGc                                                                                                                                <  2:366921/140‑1 (MQ=255)

     caaGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCt                                                                                                                              >  1:39284/1‑140 (MQ=255)

          tCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGa                                                                                                                         >  1:285932/1‑140 (MQ=255)

                      tCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTtc                                                                                                             <  2:126580/140‑1 (MQ=255)

                           ggTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTtccagct                                                                                                        <  2:285932/140‑1 (MQ=255)

                             tCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTtccagctcc                                                                                                      <  2:663850/140‑1 (MQ=255)

                             tCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTtccagctcc                                                                                                      <  2:775085/140‑1 (MQ=255)

                               aCCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTtccagctccag                                                                                                    >  2:727123/1‑140 (MQ=255)

                                ccATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTtccagctccagc                                                                                                   >  1:71512/1‑140 (MQ=255)

                                ccATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTtccagctccagc                                                                                                   >  1:158534/1‑140 (MQ=255)

                                  aTCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTtcc                                                                                                            <  1:382229/129‑1 (MQ=255)

                                  aTCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTtcc                                                                                                            >  2:382229/1‑129 (MQ=255)

                                  aTCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCcagctccagctc                                                                                                 <  2:717291/140‑1 (MQ=255)

                                    ccGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCAt                                                                                               <  1:732961/140‑1 (MQ=255)

                                           aCCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTc                                                                                        <  1:219550/140‑1 (MQ=255)

                                             cGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCAt                                                                                      <  1:365162/140‑1 (MQ=255)

                                                atatTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGc                                                                                   <  2:367636/140‑1 (MQ=255)

                                                atatTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGc                                                                                   <  2:158534/140‑1 (MQ=255)

                                                      acttacCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATaa                                                                             >  2:280278/1‑140 (MQ=255)

                                                         tacCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACa                                                                          >  1:504967/1‑140 (MQ=255)

                                                          acCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAg                                                                         <  1:280278/140‑1 (MQ=255)

                                                                aGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTc                                                                   >  2:87854/1‑140 (MQ=255)

                                                                     gcgcAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATaaa                                                              <  1:815434/140‑1 (MQ=255)

                                                                     gcgcAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATaaa                                                              <  1:599208/140‑1 (MQ=255)

                                                                     gcgcAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATaaa                                                              <  2:39284/140‑1 (MQ=255)

                                                                      cgcAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAAc                                                             >  2:523667/1‑140 (MQ=255)

                                                                          ggTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTa                                                         >  2:601332/1‑140 (MQ=255)

                                                                                tAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGa                                                   <  2:667943/140‑1 (MQ=255)

                                                                                     tGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACgcgc                                              >  1:166761/1‑140 (MQ=255)

                                                                                       cgcgcCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCtt                                            >  1:348667/1‑140 (MQ=255)

                                                                                           ccACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCAcg                                        <  2:108500/140‑1 (MQ=255)

                                                                                           ccACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCAcg                                        <  1:523667/140‑1 (MQ=255)

                                                                                              caGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCgg                                     <  2:71512/140‑1 (MQ=255)

                                                                                                gCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGaa                                   <  1:87854/140‑1 (MQ=255)

                                                                                                     gTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCa                              <  2:522253/140‑1 (MQ=255)

                                                                                                     gTTTTTCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCa                              <  2:504967/140‑1 (MQ=255)

                                                                                                      tttttCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGaa                                   >  1:767879/1‑134 (MQ=255)

                                                                                                      tttttCACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGaa                                   <  2:767879/134‑1 (MQ=255)

                                                                                                           cACCCAGGTCATCAATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCAATGccc                        >  2:754723/1‑140 (MQ=255)

                                                                                                                    catcaATGATGGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCAATGCCCTGACGCGCa               <  1:727123/140‑1 (MQ=255)

                                                                                                                            atgGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCAATGCCCTGACGCGCATATTCGTa       >  2:283583/1‑140 (MQ=255)

                                                                                                                             tgGTGCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCAATGCCCTGACGCGCATATTCGTAc      >  1:87434/1‑140 (MQ=255)

                                                                                                                                 gCGAATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCAATGCCCTGACGCGCATATTCGTACAGtt  >  1:302076/1‑140 (MQ=255)

CTCAACAAGTTCACGCAGGTGCTCCAGGGTCACCATCCGTTCAACCGGATATTTACTTACCGCCAGACGGCGCAGGTAAATAACATGCGCGCCACAGCCGAGTTTTTCACCCAGGTCATCAATGATGGTGCGGATATAAGTGCCTTTTGAGCAGTGAATTTCCAGCTCCAGCTCATTGCCTTCATGGCGAATAAACAGCAATTCATAAACGGTAATCGGACGCGCTTCACGCGGAACTTCAATGCCCTGACGCGCATATTCGTACAGTT  >  NC_000913/3312106‑3312374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A