Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,636,286	(C)_6→5	coding (1125/1203 nt)	yhiN ←	putative oxidoreductase YhiN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,636,281	0	C	.	100.0%	170.5 / NA	40	coding (1130/1203 nt)	yhiN	putative oxidoreductase YhiN
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (18/22); total (18/22)

ACTATCAAAATTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAGCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGTCACTTCGGCAGTGCGATAGC  >  NC_000913/3636148‑3636413

aCTATCAAAACTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAGcccccag                                                                                                                                <  1:607309/140‑3 (MQ=255)

  tATCAAAATTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAGcccccagcc                                                                                                                              >  2:541754/1‑136 (MQ=255)

   aTCAAAATTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCccagcca                                                                                                                            >  1:139663/1‑140 (MQ=255)

   aTCAAAATTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCccagcca                                                                                                                            >  1:433442/1‑140 (MQ=255)

    tCAAAATTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCccagccag                                                                                                                           >  2:568551/1‑140 (MQ=255)

    tCAAAATTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCccagccag                                                                                                                           >  1:465925/1‑140 (MQ=255)

    tCAAAATTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTAGAAGTTATAG‑CCCccagccag                                                                                                                           >  1:610936/1‑140 (MQ=255)

     cAAAATTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCcagccagc                                                                                                                          <  2:753593/140‑1 (MQ=255)

          ttAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTg                                                                                                                     <  2:248896/140‑1 (MQ=255)

          ttAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTg                                                                                                                     <  1:568551/140‑1 (MQ=255)

            aGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTg                                                                                                                     >  2:746957/1‑138 (MQ=255)

            aGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTg                                                                                                                     <  1:746957/138‑1 (MQ=255)

            aGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGAc                                                                                                                   >  1:359401/1‑140 (MQ=255)

                    tttAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCa                                                                                                           <  2:170406/140‑1 (MQ=255)

                          gCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGc                                                                                                     <  2:698853/140‑1 (MQ=255)

                          gCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGc                                                                                                     <  1:428915/140‑1 (MQ=255)

                             aCTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGa                                                                                                  >  1:341744/1‑140 (MQ=255)

                             aCTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGa                                                                                                  >  1:704635/1‑140 (MQ=255)

                             aCTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGa                                                                                                  >  1:835015/1‑140 (MQ=255)

                              ctctAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGAt                                                                                                 >  2:801251/1‑140 (MQ=255)

                               tctAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATg                                                                                                <  1:16626/140‑1 (MQ=255)

                               tctAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATg                                                                                                <  1:541754/140‑1 (MQ=255)

                               tctAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATg                                                                                                <  1:350623/140‑1 (MQ=255)

                                               tttAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCa                                                                                <  1:303642/140‑1 (MQ=255)

                                                        tAGGTCAGGGCGACTTTGCTGCAATTAAATCCTGCTCACAAGCCAACGAACTCGACCACGCCCACTGGAAGTTATAG‑ACCCCAGCCAGCCGGTGTCGTAAATCACTTCGCCGATGAAGTACAGCCCAGGCACATTacgcg                                                                       <  2:610936/140‑1 (MQ=255)

                                                        tAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTgcgcg                                                                       <  2:835015/140‑1 (MQ=255)

                                                        tAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTgcgcg                                                                       <  1:237436/140‑1 (MQ=255)

                                                           gTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCtt                                                                    >  1:540333/1‑140 (MQ=255)

                                                                       ttGCTGCAATCAAATCCTGCGCCCAAGCCCGCGCGCTCGGCCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTg                                                        <  2:139663/140‑1 (MQ=255)

                                                                               aTCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGt                                                >  1:571457/1‑140 (MQ=255)

                                                                                     tCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTg                                          <  2:341744/140‑1 (MQ=255)

                                                                                          cgcACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTc                                     <  2:359401/140‑1 (MQ=255)

                                                                                          cgcACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTc                                     >  1:823554/1‑140 (MQ=255)

                                                                                          cgcACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTc                                     >  1:421939/1‑140 (MQ=255)

                                                                                                  cccACGCACTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCAcgccgc                             <  2:421939/140‑1 (MQ=255)

                                                                                                         aCTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGt                      <  2:433442/140‑1 (MQ=255)

                                                                                                         aCTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGt                      <  2:874282/140‑1 (MQ=255)

                                                                                                         aCTCGACCACGCCCACTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGt                      >  1:874282/1‑140 (MQ=255)

                                                                                                                       aCTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGt                      <  2:297317/126‑1 (MQ=255)

                                                                                                                       aCTGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGt                      >  1:297317/1‑126 (MQ=255)

                                                                                                                         tGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGTCACTTCGGCAGTGCGa      <  2:540333/140‑1 (MQ=255)

                                                                                                                         tGGAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGTCACTTCGGCAGTGCGa      >  1:852761/1‑140 (MQ=255)

                                                                                                                           gAAGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGTCACTTCGGCAGTGCGATa    <  1:70443/140‑1 (MQ=255)

                                                                                                                             aGTTATAG‑CCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGTCACTTCGGCAGTGCGATAGc  <  2:465925/140‑1 (MQ=255)

ACTATCAAAATTAGCTCATTTTTAATGCGACTCTAATAATTTTCATCTTTAGGAAATAGGTCAGGACGACTTTGCTGCAATCAAATCCTGCGCACAAGCCCACGCACTCGACCACGCCCACTGGAAGTTATAGCCCCCCAGCCAGCCGGTGACGTCCATCACTTCGCCGATGAAGTACAGCCCAGGCACTTTGCGCGCTTCCATCGTCCGTGAAGAGAGTTCGTTGGTGTCCACGCCGCCGAGCGTCACTTCGGCAGTGCGATAGC  >  NC_000913/3636148‑3636413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A