Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,685,008	T→C	H204R (CAC→CGC)	pdeK ←	putative c‑di‑GMP phosphodiesterase PdeK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,685,008	0	T	C	100.0%	181.1 / NA	52	H204R (CAC→CGC)	pdeK	putative c‑di‑GMP phosphodiesterase PdeK
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (25/27); total (25/27)

ACACCGGGAAGCGCATCGCATTTTCGTTCTGTTCTTCATAATGGCGCTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGTGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAGGATAAGCGACAAAAGTAAGTAAATGGTCACTAA  >  NC_000913/3684877‑3685144

acacCGGGAAGCGCATCGCATTTTCGTTCTGTTCTTCATAATGGCGCTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACcaaca                                                                                                                                  <  2:69883/140‑1 (MQ=255)

      ggAAGCGCATCGCATTTTCGTTCTGTTCTTCATAATGGCGCTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTcc                                                                                                                            >  2:615483/1‑140 (MQ=255)

      ggAAGCGCATCGCATTTTCGTTCTGTTCTTCATAATGGCGCTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTcc                                                                                                                            <  2:259723/140‑1 (MQ=255)

      ggAAGCGCATCGCATTTTCGTTCTGTTCTTCATAATGGCGCTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTcc                                                                                                                            >  2:361456/1‑140 (MQ=255)

      ggAAGCGCATCGCATTTTCGTTCTGTTCTTCATAATGGCGCTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTc                                                                                                                             <  1:338743/139‑1 (MQ=255)

      ggAAGCGCATCGCATTTTCGTTCTGTTCTTCATAATGGCGCTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTc                                                                                                                             >  2:338743/1‑139 (MQ=255)

        aaGCGCATCGCATTTTCGTTCTGTTCTTCATAATGGCGCTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCtt                                                                                                                          <  2:470099/140‑1 (MQ=255)

             catcgcatTTTCGTTCTGTTCTTCATAATGGCGCTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTg                                                                                                                     >  1:125549/1‑140 (MQ=255)

               tcgcatTTTCGTTCTGTTCTTCATAATGGCGCTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTggg                                                                                                                   <  2:204714/140‑1 (MQ=255)

                          ttctgttctTCATAATGGCGCTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAg                                                                                                        >  1:843291/1‑140 (MQ=255)

                          ttctgttctTCATAATGGCGCTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAg                                                                                                        <  2:236213/140‑1 (MQ=255)

                                     aTAATGGCGCTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCaa                                                                                             <  1:355384/140‑1 (MQ=255)

                                         tGGCGCTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCaa                                                                                             <  2:248020/136‑1 (MQ=255)

                                         tGGCGCTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCaa                                                                                             >  1:248020/1‑136 (MQ=255)

                                         tGGCGCTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATAtt                                                                                         >  2:45726/1‑140 (MQ=255)

                                         cggCGCTGCAGCAATTGCTGGTGGAGGTTGTAACTGCGCACCACCATACCGATTTCATCCTCCTGACGCACACGCGGTAATGCCAGTTGGCGACCAACAGGCTCCTTGGCTGCGATGCCGTTAAGTTCGCGCGCAATAtt                                                                                         >  2:537283/2‑140 (MQ=255)

                                            cgcTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAata                                                                                           <  1:853745/135‑1 (MQ=255)

                                            cgcTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAata                                                                                           >  2:853745/1‑135 (MQ=255)

                                              cTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAg                                                                                                >  1:266883/1‑128 (MQ=255)

                                              cTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAg                                                                                                <  2:454580/128‑1 (MQ=255)

                                              cTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAg                                                                                                >  1:454580/1‑128 (MQ=255)

                                              cTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAg                                                                                                <  2:266883/128‑1 (MQ=255)

                                                 cagcaATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACg                                                                                 >  1:680149/1‑140 (MQ=255)

                                                            ggTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATc                                                                      >  1:154695/1‑140 (MQ=255)

                                                             gTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCa                                                                     <  1:615483/140‑1 (MQ=255)

                                                             gTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCa                                                                     >  2:645048/1‑140 (MQ=255)

                                                             gTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCa                                                                     >  2:615816/1‑140 (MQ=255)

                                                                gAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGc                                                                  <  2:528486/140‑1 (MQ=255)

                                                                 aGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCg                                                                 >  2:727744/1‑140 (MQ=255)

                                                                               cACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCacc                                                   >  2:82288/1‑138 (MQ=255)

                                                                                aCCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCt                                                  >  2:840455/1‑140 (MQ=255)

                                                                                  cAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGa                                                <  1:727744/140‑1 (MQ=255)

                                                                                       tACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCg                                           <  2:843291/140‑1 (MQ=255)

                                                                                       tACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCg                                           <  2:68138/140‑1 (MQ=255)

                                                                                            aTTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGt                                      >  2:833661/1‑140 (MQ=255)

                                                                                              ttCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCa                                    >  1:248005/1‑140 (MQ=255)

                                                                                                cATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAgg                                  <  1:45726/140‑1 (MQ=255)

                                                                                                   cGTCCTGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAGGATa                               <  1:392364/140‑1 (MQ=255)

                                                                                                        tGATGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAGGATAAGCGa                          <  2:478348/140‑1 (MQ=255)

                                                                                                           tGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAGGATAAGCGACaa                       <  1:361456/140‑1 (MQ=255)

                                                                                                           tGCAGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAGGATAAGCGACaa                       <  2:223253/140‑1 (MQ=255)

                                                                                                              aGACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAGGATAAGCGACAAaag                    <  2:125549/140‑1 (MQ=255)

                                                                                                               gACGCGGTAATGTGAGTTGGCGACCAACAAGCTCCTTGGTTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTTATGCACCGGCTGTTGGCGACTGTCAGGTTGAGAGACAAaaga                   >  2:350854/1‑139 (MQ=255)

                                                                                                               gACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAGGATAAGCGACaa                       >  2:480097/1‑136 (MQ=255)

                                                                                                               gACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAGGATAAGCGACaa                       <  1:480097/136‑1 (MQ=255)

                                                                                                               gACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAGGATAAGCGACAAaagt                   >  2:819165/1‑140 (MQ=255)

                                                                                                               gACGCGGTAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAGGATAAGCGACAAaagt                   >  1:850248/1‑140 (MQ=255)

                                                                                                                      tAATGCCAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAGGATAAGCGACAAAAGTAAGTaaa            <  2:850248/140‑1 (MQ=255)

                                                                                                                            cAGTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAGGATAAGCGACAAAAGTAAGTAAATGGTCa      <  1:833661/140‑1 (MQ=255)

                                                                                                                              gTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAGGATAAGCGACAAAAGTAAGTAAATGGTCACt    >  1:447846/1‑140 (MQ=255)

                                                                                                                              gTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAGGATAAGCGACAAAAGTAAGTAAATGGTCACt    >  2:327175/1‑140 (MQ=255)

                                                                                                                              gTTGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAGGATAAGCGACAAAAGTAAGTAAATGGTCACt    <  2:14579/140‑1 (MQ=255)

                                                                                                                                tGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAGGATAAGCGACAAAAGTAAGTAAATGGTCACTaa  >  2:477549/1‑140 (MQ=255)

                                                                                                                                tGGCGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAGGATAAGCGACAAAAGTAAGTAAATGGTCACTaa  <  1:840455/140‑1 (MQ=255)

ACACCGGGAAGCGCATCGCATTTTCGTTCTGTTCTTCATAATGGCGCTGCAGCAATTGCTGGTTGAGGTTGTAACTGCGCACCAACATACCGATTTCATCGTCCTGATGCAGACGCGGTAATGCCAGTTGGTGACCAACAAGCTCCTTGGCTGGGATGGCGTTAAGTTCGCGAGCAATATTGCGTAACGGATGCAAAATCAGGCGGTTAATGCACCAGCTGATGGCGACGGTCAGGATAAGCGACAAAAGTAAGTAAATGGTCACTAA  >  NC_000913/3684877‑3685144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A