Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,694,567	A→G	W224R (TGG→CGG)	bcsA ←	cellulose synthase catalytic subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,694,567	0	A	G	95.8%	156.8 / ‑2.4	48	W224R (TGG→CGG)	bcsA	cellulose synthase catalytic subunit
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (20/26); total (21/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

ATATCTTTCGGCAATGGCACCGGCTGACGATTCAGCGGCCATACTACCTGGAAGTAGCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGCTACCCCCCACAGCAGCAT  >  NC_000913/3694431‑3694696

atatCTTTCGGCAATGGCACCGGCTGACGATTCAGCGGCCATACTACCTGGAAGTAGCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGtt                                                                                                                                <  2:626834/140‑1 (MQ=255)

atatCTTTCGGCAATGGCACCGGCTGACGATTCAGCGGCCATACTACCTGGAAGTAGCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGACCGTCCCGGtt                                                                                                                                <  2:710743/140‑1 (MQ=255)

  atCTTTCGGCAATGGCACCGGCTGACGATTCAGCGGCCATACTACCTGGAAGTAGCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCa                                                                                                                              <  1:808266/140‑1 (MQ=255)

   tCTTTCGGCAATGGCACCGGCTGACGATTCAGCGGCCATACTACCTGGAAGTAGCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAg                                                                                                                             <  2:510103/140‑1 (MQ=255)

     tttCGGCAATGGCACCGGCTGACGATTCAGCGGCCATACTACCTGGAAGTAACCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCg                                                                                                                           <  2:775511/140‑1 (MQ=255)

      ttCGGCAATGGCACCGGCTGACGATTCAGCGGCCATACTACCTGGAAGTAGCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGt                                                                                                                          >  2:734708/1‑140 (MQ=255)

         ggCAATGGCACCGGCTGACGATTCAGCGGCCATACTACCTGGAAGTAGCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTaga                                                                                                                       >  1:371895/1‑140 (MQ=255)

         ggCAATGGCACCGGCTGACGATTCAGCGGCCATACTACCTGGAAGTAGCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTaga                                                                                                                       >  1:422940/1‑140 (MQ=255)

                gCACCGGCTGACGATTCAGCGGCCATACTACCTGGAAGTAGCAGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTaa                                                                                                                <  1:96773/140‑1 (MQ=255)

                  aCCGGCTGACGATTCAGCGGCCATACTACCTGGAAGTAGCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACg                                                                                                              <  1:118221/140‑1 (MQ=255)

                       cTGACGATTCAGCGGCCATACTACCTGGAAGTAGCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGccacc                                                                                                         <  1:734708/140‑1 (MQ=255)

                                 aGCGGCCATACTACCTGGAAGTAGCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACg                                                                                               <  1:201066/140‑1 (MQ=255)

                                         tactacCTGGAAGTAGCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGaaa                                                                                       <  1:243459/140‑1 (MQ=255)

                                                   aaGTAGCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGa                                                                             >  2:342234/1‑140 (MQ=255)

                                                      tAGCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAg                                                                          >  1:282050/1‑140 (MQ=255)

                                                      tAGCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAg                                                                          <  2:212079/140‑1 (MQ=255)

                                                      tAGCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAg                                                                          >  2:609646/1‑140 (MQ=255)

                                                        gCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGca                                                                        >  1:481426/1‑140 (MQ=255)

                                                        gCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGca                                                                        >  2:624446/1‑140 (MQ=255)

                                                            aGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAAt                                                                    <  2:371895/140‑1 (MQ=255)

                                                             gCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATc                                                                   <  1:342234/140‑1 (MQ=255)

                                                                         tCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGcc                                                       >  2:41049/1‑140 (MQ=255)

                                                                           cACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCga                                                     >  2:370360/1‑140 (MQ=255)

                                                                             cgcgTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCgaga                                                   >  2:596151/1‑140 (MQ=255)

                                                                                   cGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGcc                                             <  2:481426/140‑1 (MQ=255)

                                                                                   cGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGcc                                             >  2:125786/1‑140 (MQ=255)

                                                                                        cAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGc                                        >  2:569841/1‑140 (MQ=255)

                                                                                           cGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATc                                     <  1:636038/140‑1 (MQ=255)

                                                                                              agagCAGAATAAGCCCGCACACCAGGCTGACCGGCTCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGATCAGCGACAGCACAATCACCATTAGCGCTGAGAAGCGCCCCGGCATccgc                                  <  2:815475/140‑1 (MQ=255)

                                                                                                 gCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGt                               <  2:422940/140‑1 (MQ=255)

                                                                                                         aGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAg                       <  1:624446/140‑1 (MQ=255)

                                                                                                         aGCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAg                       <  1:596151/140‑1 (MQ=255)

                                                                                                          gCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAgc                      >  2:147203/1‑140 (MQ=255)

                                                                                                          gCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAgc                      >  2:43441/1‑140 (MQ=255)

                                                                                                          gCCCGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAgc                      >  2:604763/1‑140 (MQ=255)

                                                                                                            ccGCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAgcgc                    >  2:165653/1‑140 (MQ=255)

                                                                                                              gCACACCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGCTa                  <  1:370360/140‑1 (MQ=255)

                                                                                                               cacaCCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATc                         >  2:39461/1‑132 (MQ=255)

                                                                                                               cacaCCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATc                         <  1:39461/132‑1 (MQ=255)

                                                                                                               cacaCCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGCTa                  >  2:331226/1‑139 (MQ=255)

                                                                                                               cacaCCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGCTa                  <  1:331226/139‑1 (MQ=255)

                                                                                                                acacCAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGCTAcc                <  1:165653/140‑1 (MQ=255)

                                                                                                                   ccAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGCTAccccc             <  1:43441/140‑1 (MQ=255)

                                                                                                                    cAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGCTAcc                <  1:122061/136‑1 (MQ=255)

                                                                                                                    cAGGCTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGCTAcc                >  2:122061/1‑136 (MQ=255)

                                                                                                                        cTGACCGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGCTACCCCCCAcag        <  2:282050/140‑1 (MQ=255)

                                                                                                                         tGACCGGATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGCTACCCCCCAcagc       <  1:609646/140‑1 (MQ=255)

                                                                                                                             cGGATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGCTACCCCCCACAgcagca   >  1:33945/1‑140 (MQ=255)

                                                                                                                              ggATCGTCCCGGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGCTACCCCCCACAGCAGCAt  <  1:760794/140‑1 (MQ=255)

ATATCTTTCGGCAATGGCACCGGCTGACGATTCAGCGGCCATACTACCTGGAAGTAGCCGAGCACCAGCACAATCCACGCGTACGTTTCAGCGAAGAGCAGAATAAGCCCGCACACCAGGCTGACCGGATCGTCCCAGTTCAGCGTAGAGGTGTAACGCCACCAGATATAACGGCAAGAAACGGTCAGCGACAGCACAATCAACATTAGCGCCGAGAAGCGCCCCGGCATCCGCCGTACGATCAGCGCTACCCCCCACAGCAGCAT  >  NC_000913/3694431‑3694696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A