Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,743,007	(A)_5→4	coding (275/999 nt)	yiaK →	2,3‑diketo‑L‑gulonate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,743,003	0	A	.	100.0%	184.5 / NA	43	coding (271/999 nt)	yiaK	2,3‑diketo‑L‑gulonate reductase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (26/17); total (26/17)

GCGTTAATCGTTTCCCTCGTTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCGAAAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGGAAAAAGGCTATA  >  NC_000913/3742866‑3743129

gCGTTAATCGTTTCCCTCGTTTCATTCAACAACTGGAAAACGGCGGTATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCGaaa                                                                                                                              <  2:142015/140‑1 (MQ=255)

           ttCCCTCGTTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGAt                                                                                                                  >  1:870710/1‑140 (MQ=255)

           ttCCCTCGTTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGAt                                                                                                                  >  1:495283/1‑140 (MQ=255)

             cccTCGTTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATcg                                                                                                                <  2:870710/140‑1 (MQ=255)

                       attcaACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGaa                                                                                                      >  2:388502/1‑140 (MQ=255)

                           aacaacTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTgg                                                                                                  <  2:156909/140‑1 (MQ=255)

                             caacTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCt                                                                                                >  1:554259/1‑140 (MQ=255)

                              aacTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTg                                                                                               <  1:423010/140‑1 (MQ=255)

                                 tGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCg                                                                                            >  2:378278/1‑140 (MQ=255)

                                   gAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGAt                                                                                          >  2:708375/1‑140 (MQ=255)

                                    aaaaCGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATc                                                                                         <  2:554259/140‑1 (MQ=255)

                                     aaaCGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCa                                                                                        <  2:495283/140‑1 (MQ=255)

                                     aaaCGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCa                                                                                        <  2:472168/140‑1 (MQ=255)

                                        cGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACgg                                                                                     <  2:112453/140‑1 (MQ=255)

                                        cGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACgg                                                                                     <  1:708375/140‑1 (MQ=255)

                                          gCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTa                                                                                   <  2:511812/140‑1 (MQ=255)

                                               atCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGt                                                                              <  2:835149/140‑1 (MQ=255)

                                                   ttCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCtgg                                                                          <  1:496696/140‑1 (MQ=255)

                                                        gATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCtgg                                                                          >  1:820761/1‑135 (MQ=255)

                                                        gATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCtgg                                                                          <  2:820761/135‑1 (MQ=255)

                                                        gATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCa                                                                     >  1:232392/1‑140 (MQ=255)

                                                         aTGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCAc                                                                    >  1:669662/1‑140 (MQ=255)

                                                         aTGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCAc                                                                    >  1:457464/1‑140 (MQ=255)

                                                         aTGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCAc                                                                    >  1:432265/1‑140 (MQ=255)

                                                         aTGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCAc                                                                    >  2:445867/1‑140 (MQ=255)

                                                           gCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCtgg                                                                          >  1:148467/1‑132 (MQ=255)

                                                           gCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCtgg                                                                          <  2:148467/132‑1 (MQ=255)

                                                              caacccaaACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGTCAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCtgg                                                                          <  2:612474/129‑1 (MQ=255)

                                                              caacccaaACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCtgg                                                                          >  1:612474/1‑129 (MQ=255)

                                                                acccaaACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGCGGCACTACGTaa                                                             >  2:194534/1‑140 (MQ=255)

                                                                 cccaaACG‑‑TAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCACTACGTAATGc                                                          <  2:455379/140‑1 (MQ=255)

                                                                    aaaCGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCACTACGTAATGcc                                                         <  1:378278/140‑1 (MQ=255)

                                                                           taACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACt                                                  >  2:663572/1‑140 (MQ=255)

                                                                           taACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACt                                                  >  1:672464/1‑140 (MQ=255)

                                                                            aaCCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACTg                                                 >  1:356546/1‑140 (MQ=255)

                                                                                  ccTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATgcg                                           >  1:127294/1‑140 (MQ=255)

                                                                                                       ggACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCa                      >  1:288104/1‑140 (MQ=255)

                                                                                                       ggACGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCa                      >  2:2511/1‑140 (MQ=255)

                                                                                                         aCGCCCAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAgg                    <  2:669662/140‑1 (MQ=255)

                                                                                                             ccAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAggcggc                >  1:277643/1‑140 (MQ=255)

                                                                                                             ccAGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAggcggc                >  1:208222/1‑140 (MQ=255)

                                                                                                               aGCGTTCGATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGcggcgg              >  2:188542/1‑140 (MQ=255)

                                                                                                                 cgagcGACCGGTAACCAGAAAGAGAAAAAGATGATGGTAGGAGCCATTGATTTGGCTGCTGATAGCGGTATTGGTCTGGTGGCCCTACGTAATGCCACCAACTGGATGCGCGGCGGCAGATACGGCTGGCAGGCGGCGGa             <  2:505363/136‑1 (MQ=255)

                                                                                                                      gATCGGTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGACTGCCGATCACGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGGAAAAAgg       >  1:250023/1‑140 (MQ=255)

                                                                                                                           gTAACCTGACAGCG‑AAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGGAAAAAGGCtata  >  1:428110/1‑140 (MQ=255)

GCGTTAATCGTTTCCCTCGTTTCATTCAACAACTGGAAAACGGCGATATCATTCCTGATGCCCAACCCAAACGTATAACCAGCCTCGGCGCAATTGAACAGTGGGACGCCCAGCGTTCGATCGGTAACCTGACAGCGAAAAAGATGATGGATCGCGCCATTGAACTGGCTGCCGATCACGGTATTGGTCTGGTGGCACTACGTAATGCCAACCACTGGATGCGCGGCGGCAGCTACGGCTGGCAGGCGGCGGAAAAAGGCTATA  >  NC_000913/3742866‑3743129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A