Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F28 I1 R1 227 52.0 1764248 99.3% 1751898 138.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,873,948 C→T G176D (GGT→GAT)  dgoK ← 2‑dehydro‑3‑deoxygalactonokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,873,9480CT100.0% 134.0 / NA 39G176D (GGT→GAT) dgoK2‑dehydro‑3‑deoxygalactonokinase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/24);  total (15/24)

CCTGTTCGCGGGGAAGTGTTCCCAGCACATGCGAGGCGCGAACTTCAAAAAGCTGCGGCAATATGGCGGGCGTATTAAGACCACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGA  >  NC_000913/3873814‑3874081
                                                                                                                                      |                                                                                                                                     
ccTGTTCGCGGGGAAGTGTTCCCAGCACATGCGAGGCGCGAACTTCAAAAAGCTGCGGCAATATGGCGGGCGTATTAAGACCACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTgcg                                                                                                                                  <  1:627405/140‑1 (MQ=255)
   gTTCGCGGGGAAGTGTTCCCAGCACATGCGAGGCGCGAACTTCAAAAAGCTGCGGCAATATGGCGGGCGTATTAAGACCACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCca                                                                                                                               <  1:317384/140‑1 (MQ=255)
     tCGCGGGGAAGTGTTCCCAGCACATGCGAGGCGCGAACTTCAAAAAGCTGCGGCAATATGGCGGGCGTATTAAGACCACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCcaat                                                                                                                             <  1:567241/140‑1 (MQ=255)
       gcgGGGAAGTGTTCCCAGCACATGCGAGGCGCGAACTTCAAAAAGCTGCGGCAATATGGCGGGCCTATTAAGACCACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCcaatca                                                                                                                           <  2:701057/140‑1 (MQ=255)
          gggAAGTGTTCCCAGCACATGCGAGGCGCGAACTTCAAAAAGCTGCGGCAATATGGCGGGCGTATTAAGACCACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATg                                                                                                                        <  1:272266/140‑1 (MQ=255)
          aggAAGTGTTCCCAGCACATGCGAGGCGCGAACTTCAAAAAGCTGCGGCAATATGGCGGGCGTATTAAGACCACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATg                                                                                                                        <  1:310145/139‑1 (MQ=255)
            gAAGTGTTCCCAGCACATGCGAGGCGCGAACTTCAAAAAGCTGCGGCAATATGGCGGGCGTATTAAGACCACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAg                                                                                                                      <  1:858379/140‑1 (MQ=255)
              aGTGTTCCCAGCACATGCGAGGCGCGAACTTCAAAAAGCTGCGGCAATATGGCGGGCGGGTTAAAAACACCCCCCAAGCCCGCTGGGGAGGGATCGGCAGAGGTTTTCTGCGGCGGGCAAACTGCGCCCATCAATGAggg                                                                                                                    >  2:567241/1‑138 (MQ=255)
                 gtTCCCAGCACATGCGAGGCGCGAACTTCAAAAAGCTGCGGCAATATGGCGGGCGTATTAAGACCACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGAtt                                                                                                                 <  2:297470/140‑1 (MQ=255)
                  ttCCCAGCACATGCGAGGCGCGAACTTCAAAAAGCTGCGGCAATATGGCGGGCGTATTAAGACCACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGAttt                                                                                                                <  1:452591/140‑1 (MQ=255)
                       aGCACATGCGAGGCGCGAACTTCAAAAAGCTGCGGCAATATGGCGGGCGGATTTAAACCACCCCCCAGGCCAGCTGTGAAGGGAGCGGCAGAGGTTTTCTGCGGCGGGGAAACTGCGCCCAACAAAAAGGGAttttatcc                                                                                                           >  2:310145/1‑135 (MQ=255)
                                gAGGCGCGAACTTCAAAAAGCTGCGGCAATATGGCGGGCGTATTAAGACCACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAatgatg                                                                                                  <  1:624948/140‑1 (MQ=255)
                                       gAACTTCAAAAAGCTGCGGCAATATGGCGGGCGTATTAAGACCACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCa                                                                                           <  1:323202/140‑1 (MQ=255)
                                       gAACTTCAAAAAGCTGCGGCAATATGGCGGGCGTATTAAGACCACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCa                                                                                           <  2:790097/140‑1 (MQ=255)
                                                 aaGCTGCGGCAATATGGCGGGCGTATTAAGACCACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGtcatca                                                                                 <  1:92772/140‑1 (MQ=255)
                                                 aaGCTGCGGCAATATGGCGGGCGTATTAAGACCACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGtcatca                                                                                 <  1:323680/140‑1 (MQ=255)
                                                       cGGCAATATGGCGGGCGTATTAAGAACACCCCCCAAGCCAGCCGTGGAGGCATCGGGAGAGGTTTTCTGCGGCGGCGAAACTTCGCCCAACAAAAAGTGAGTTTAAAATAAAAGATGTAATTCACCCGTCATCACGGGGc                                                                           >  2:626747/1‑140 (MQ=255)
                                                                            aaGACCACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGc                                                      >  1:864730/1‑140 (MQ=255)
                                                                               aCCACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTAt                                                   >  2:695767/1‑140 (MQ=255)
                                                                                 cACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCt                                                         <  1:705317/132‑1 (MQ=255)
                                                                                 cACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCt                                                         >  2:705317/1‑132 (MQ=255)
                                                                                   cGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGc                                               >  2:34854/1‑140 (MQ=255)
                                                                                   cGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGc                                               >  2:410756/1‑140 (MQ=255)
                                                                                   cGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGc                                               >  1:742096/1‑140 (MQ=255)
                                                                                   cGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGc                                               >  2:732008/1‑140 (MQ=255)
                                                                                   cGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGc                                               >  1:19646/1‑140 (MQ=255)
                                                                                   cGCTCAAGGCCAGCTGTGAAGACATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGc                                               >  2:628772/1‑140 (MQ=255)
                                                                                        aaGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCa                                          >  1:664015/1‑140 (MQ=255)
                                                                                         aGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTg                                            <  1:76298/137‑1 (MQ=255)
                                                                                         aGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTg                                            >  2:76298/1‑137 (MQ=255)
                                                                                              aGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCAtt                                    >  2:520496/1‑140 (MQ=255)
                                                                                                    gAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGAATTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAAt                              <  1:248926/140‑1 (MQ=255)
                                                                                                         cATCGGCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGtt                         <  2:833534/140‑1 (MQ=255)
                                                                                                             ggCAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTcccg                     >  2:622232/1‑138 (MQ=255)
                                                                                                               cAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGc                   <  2:310135/140‑1 (MQ=255)
                                                                                                               cAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGc                   <  1:732008/140‑1 (MQ=255)
                                                                                                               cAGAGTTTTCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGc                   <  1:626747/140‑1 (MQ=255)
                                                                                                                      ttCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACAt            <  2:864730/140‑1 (MQ=255)
                                                                                                                       tCCTGCGGCGGCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATa           >  1:814562/1‑140 (MQ=255)
                                                                                                                            cggcggCAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAagag      <  1:837233/140‑1 (MQ=255)
                                                                                                                              gcggcAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAagagag    >  1:791355/1‑140 (MQ=255)
                                                                                                                                ggcAAATCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTACGGGCATGACATAAAGAGagga  <  1:410756/140‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                     
CCTGTTCGCGGGGAAGTGTTCCCAGCACATGCGAGGCGCGAACTTCAAAAAGCTGCGGCAATATGGCGGGCGTATTAAGACCACGCTCAAGGCCAGCTGTGAAGGCATCGGCAGAGTTTTCCTGCGGCGGCAAACCTGCGCCAATCAATGAGTGATTTAACAGTAAATGATGTAATTCACCGGTCATCACGGTGCGAAAATCGTTGATTTGCTGGCTATCGGCCTGCACCCATTTGCAATGGGTTCCGGGCATGACATAAAGAGAGGA  >  NC_000913/3873814‑3874081

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A