Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,954,120	C→A	Q215K (CAG→AAG)	ilvD →	dihydroxy‑acid dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,954,120	0	C	A	100.0%	190.4 / NA	54	Q215K (CAG→AAG)	ilvD	dihydroxy‑acid dehydratase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (30/24); total (30/24)

AGGGCGCAGACCCGAAAGTATCTGACTCCCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATATC  >  NC_000913/3953986‑3954254

aGGGCGCAGACCCGAAAGTATCTGACTCCCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCg                                                                                                                                   >  1:555848/1‑140 (MQ=255)

 gggCGCAGACCCGAAAGTATCTGACTCCCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCgg                                                                                                                                  >  1:271322/1‑140 (MQ=255)

   gcgcAGACCCGAAAGTATCTGACTCCCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGc                                                                                                                                >  1:605422/1‑140 (MQ=255)

    cgcAGACCCGAAAGTATCTGACTCCCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCa                                                                                                                               >  1:854562/1‑140 (MQ=255)

         aCCCGAAAGTATCTGACTCCCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGc                                                                                                                          <  2:287026/140‑1 (MQ=255)

          cccGAAAGTATCTGACTCCCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCt                                                                                                                         <  2:605422/140‑1 (MQ=255)

          cccGAAAGTATCTGACTCCCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCt                                                                                                                         <  2:800617/140‑1 (MQ=255)

                     cTGACTCCCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGctgctg                                                                                                                 <  1:837731/137‑1 (MQ=255)

                     cTGACTCCCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGctgctg                                                                                                                 >  2:837731/1‑137 (MQ=255)

                     cTGACTCCCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCa                                                                                                              >  2:279677/1‑140 (MQ=255)

                            ccAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACg                                                                                                       <  1:478709/140‑1 (MQ=255)

                            ccAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCCCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACg                                                                                                       >  1:272763/1‑140 (MQ=255)

                              agagCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGcc                                                                                                     >  1:864752/1‑140 (MQ=255)

                                    aTCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGt                                                                                               >  2:623622/1‑140 (MQ=255)

                                    aTCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGt                                                                                               >  1:394563/1‑140 (MQ=255)

                                      cAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTaa                                                                                             >  1:662925/1‑140 (MQ=255)

                                      cAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTaa                                                                                             >  1:27207/1‑140 (MQ=255)

                                          ttGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAagcag                                                                                         <  1:781516/140‑1 (MQ=255)

                                          ttGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAagcag                                                                                         <  2:27207/140‑1 (MQ=255)

                                          ttGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAagcag                                                                                         <  2:625982/140‑1 (MQ=255)

                                          ttGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAagcag                                                                                         <  1:279677/140‑1 (MQ=255)

                                          ttGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAagcag                                                                                         >  2:862474/1‑140 (MQ=255)

                                          ttGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAagcag                                                                                         <  2:864752/140‑1 (MQ=255)

                                             aaCGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTg                                                                                      >  2:822882/1‑140 (MQ=255)

                                             aaCGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTg                                                                                      <  1:386848/140‑1 (MQ=255)

                                                   ccGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAagcag                                                                                         <  1:38155/131‑1 (MQ=255)

                                                   ccGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAagcag                                                                                         >  1:850571/1‑131 (MQ=255)

                                                   ccGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAagcag                                                                                         <  2:850571/131‑1 (MQ=255)

                                                   ccGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAagcag                                                                                         >  2:38155/1‑131 (MQ=255)

                                                   ccGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCtt                                                                                >  2:103198/1‑140 (MQ=255)

                                                    cgcgTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTa                                                                               <  1:103198/140‑1 (MQ=255)

                                                         gtCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCt                                                                          <  2:394563/140‑1 (MQ=255)

                                                                  gCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGc                                                                 >  1:569408/1‑140 (MQ=255)

                                                                   cGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCa                                                                >  2:484853/1‑140 (MQ=255)

                                                                     gTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCAtt                                                              >  1:710595/1‑140 (MQ=255)

                                                                             tCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAAtt                                                      >  2:827702/1‑140 (MQ=255)

                                                                                 ggATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGAcc                                                  <  2:710595/140‑1 (MQ=255)

                                                                                  gATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCa                                                 <  2:569408/140‑1 (MQ=255)

                                                                                   aTGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCaa                                                >  1:273020/1‑140 (MQ=255)

                                                                                      tttACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACg                                             >  1:544152/1‑140 (MQ=255)

                                                                                        tACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCa                                                 <  1:139256/134‑1 (MQ=255)

                                                                                        tACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCa                                                 >  2:139256/1‑134 (MQ=255)

                                                                                                aCTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAg                                   >  1:433098/1‑140 (MQ=255)

                                                                                                    aaTGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCaaa                               <  1:862474/140‑1 (MQ=255)

                                                                                                      tGAACTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAac                             >  1:494336/1‑140 (MQ=255)

                                                                                                        aaCTGCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAacga                           <  2:271322/140‑1 (MQ=255)

                                                                                                            gCCTGACCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGaa                       >  2:35848/1‑140 (MQ=255)

                                                                                                                 aCCGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGc                  >  2:571838/1‑140 (MQ=255)

                                                                                                                  ccGAAGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCa                 <  2:544152/140‑1 (MQ=255)

                                                                                                                     aaGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTg              <  2:273020/140‑1 (MQ=255)

                                                                                                                     aaGCGCTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTg              <  2:718758/140‑1 (MQ=255)

                                                                                                                       gcgcTGGGCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGcc            >  1:370332/1‑140 (MQ=255)

                                                                                                                            gggCCTGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTa       <  2:370332/140‑1 (MQ=255)

                                                                                                                                 tGTCGAAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATATc  >  1:75300/1‑140 (MQ=255)

AGGGCGCAGACCCGAAAGTATCTGACTCCCAGAGCGATCAGGTTGAACGTTCCGCGTGTCCGACCTGCGGTTCCTGCTCCGGGATGTTTACCGCTAACTCAATGAACTGCCTGACCGAAGCGCTGGGCCTGTCGCAGCCGGGCAACGGCTCGCTGCTGGCAACCCACGCCGACCGTAAGCAGCTGTTCCTTAATGCTGGTAAACGCATTGTTGAATTGACCAAACGTTATTACGAGCAAAACGACGAAAGTGCACTGCCGCGTAATATC  >  NC_000913/3953986‑3954254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A