Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,261,093	A→G	G165G (GGA→GGG)	yjbM →	uncharacterized protein YjbM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,261,093	0	A	G	94.1%	224.7 / 6.5	68	coding (495/708 nt)	yjbM	uncharacterized protein YjbM
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (33/31); minor base . (2/2); total (35/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

GTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAAC  >  NC_000913/4260957‑4261227

gTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGaa                                                                                                                                     >  2:582109/1‑140 (MQ=255)

gTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGaa                                                                                                                                     <  1:550050/140‑1 (MQ=255)

gTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGaa                                                                                                                                     >  2:132222/1‑140 (MQ=255)

  gCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGaaa                                                                                                                                    <  1:611494/139‑1 (MQ=255)

  gCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGaaa                                                                                                                                    >  2:611494/1‑139 (MQ=255)

    cTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGa                                                                                                                                 >  1:644448/1‑140 (MQ=255)

    cTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGa                                                                                                                                 >  1:251577/1‑140 (MQ=255)

      aCGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAAt                                                                                                                               >  2:62772/1‑140 (MQ=255)

      aCGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAAt                                                                                                                               >  1:472728/1‑140 (MQ=255)

          aGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTg                                                                                                                           >  1:387378/1‑140 (MQ=255)

           gATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGa                                                                                                                          >  1:174500/1‑140 (MQ=255)

            aTTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGAt                                                                                                                         >  2:676422/1‑140 (MQ=255)

             tttATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATg                                                                                                                        <  1:132222/140‑1 (MQ=255)

             tttATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATg                                                                                                                        >  2:654630/1‑140 (MQ=255)

              ttATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATgg                                                                                                                       <  1:62772/140‑1 (MQ=255)

              ttATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATgg                                                                                                                       <  1:459809/140‑1 (MQ=255)

                aTCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCt                                                                                                                     <  1:676422/140‑1 (MQ=255)

                 tCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTa                                                                                                                    <  2:212543/140‑1 (MQ=255)

                  cAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTAt                                                                                                                   <  1:343939/140‑1 (MQ=255)

                      aaCAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGG‑GAAATGAATTTTGATGGCTATCTGGa                                                                                                              >  2:171933/1‑140 (MQ=255)

                         aGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATa                                                                                                            <  2:127769/140‑1 (MQ=255)

                          gtgtATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATaa                                                                                                           <  1:752292/140‑1 (MQ=255)

                           tgtATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAg                                                                                                          >  2:713616/1‑140 (MQ=255)

                           tgtATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAg                                                                                                          >  2:845710/1‑140 (MQ=255)

                               tatTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGt                                                                                                      <  1:549306/140‑1 (MQ=255)

                               tatTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGt                                                                                                      <  1:845710/140‑1 (MQ=255)

                                 ttAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTca                                                                                                    <  2:174500/140‑1 (MQ=255)

                                  tAATGAAAATAATGAGAGCTACATGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTcac                                                                                                   <  1:625573/140‑1 (MQ=255)

                                     tGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATc                                                                                                >  1:674134/1‑140 (MQ=255)

                                     tGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATc                                                                                                >  2:434562/1‑140 (MQ=255)

                                     tGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATc                                                                                                >  2:1993/1‑140 (MQ=255)

                                     tGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATc                                                                                                >  1:653623/1‑140 (MQ=255)

                                     tGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATc                                                                                                >  2:761827/1‑140 (MQ=255)

                                         aataatGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGt                                                                                            <  1:186521/140‑1 (MQ=255)

                                         aataatGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGt                                                                                            <  2:472728/140‑1 (MQ=255)

                                         aataatGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGt                                                                                            >  2:466975/1‑140 (MQ=255)

                                         aataatGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGt                                                                                            <  1:630246/140‑1 (MQ=255)

                                           taatGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGtga                                                                                          <  1:654630/140‑1 (MQ=255)

                                            aatGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGtgaa                                                                                         <  1:713616/140‑1 (MQ=255)

                                              tGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGtgaatg                                                                                       >  1:669951/1‑140 (MQ=255)

                                                 gagCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAg                                                                                    <  1:1993/140‑1 (MQ=255)

                                                   gCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGa                                                                                  >  2:256473/1‑140 (MQ=255)

                                                        cTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCt                                                                             <  1:761827/140‑1 (MQ=255)

                                                        cTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCt                                                                             <  1:466975/140‑1 (MQ=255)

                                                                  aGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGat                                                                   >  1:212589/1‑140 (MQ=255)

                                                                    tttGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGatat                                                                 >  2:340802/1‑140 (MQ=255)

                                                                    tttGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGatat                                                                 >  2:186826/1‑140 (MQ=255)

                                                                            aTTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCa                                                         <  2:69360/140‑1 (MQ=255)

                                                                              tATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCaaa                                                       <  1:409245/140‑1 (MQ=255)

                                                                                      cGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCgaga                                               <  2:212589/140‑1 (MQ=255)

                                                                                        tGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGAtg                                             <  1:434562/140‑1 (MQ=255)

                                                                                             tGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGG‑GAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGcac                                       <  1:171933/140‑1 (MQ=255)

                                                                                                       ccTGGGAGAGATGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACaattaa                              >  2:838911/1‑140 (MQ=255)

                                                                                                         tGGGAGAGATGAAGCGTCTGGCTGATGGGGG‑GAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGcac                                       >  2:741148/1‑128 (MQ=255)

                                                                                                         tGGGAGAGATGAAGCGTCTGGCTGATGGGGG‑GAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGcac                                       <  1:741148/128‑1 (MQ=255)

                                                                                                            gagagaTGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGc                         >  2:458279/1‑140 (MQ=255)

                                                                                                                 aTGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTcgc                    >  2:583708/1‑140 (MQ=255)

                                                                                                                 aTGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTcgc                    >  1:371506/1‑140 (MQ=255)

                                                                                                                  tGAAGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTcgcg                   <  2:669951/140‑1 (MQ=255)

                                                                                                                    aaGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGaa                 <  1:583708/140‑1 (MQ=255)

                                                                                                                     aGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAg                >  2:524933/1‑140 (MQ=255)

                                                                                                                     aGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAg                >  1:549763/1‑140 (MQ=255)

                                                                                                                     aGCGTCTGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAg                >  2:438244/1‑140 (MQ=255)

                                                                                                                         tCTGGCTGATGGGGGGGATATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGt            >  1:525185/1‑140 (MQ=255)

                                                                                                                           tGGCTGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGttt          >  2:104164/1‑140 (MQ=255)

                                                                                                                               tGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGAcc      <  2:371506/140‑1 (MQ=255)

                                                                                                                               tGATGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGAcc      <  2:387378/140‑1 (MQ=255)

                                                                                                                                 aTGGGGGGGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCa    <  1:104164/140‑1 (MQ=255)

                                                                                                                                   ggggggGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAAc  <  2:653623/140‑1 (MQ=255)

GTGCCTACGCAGATTTATCAGAAACAGTGTATATTAATGAAAATAATGAGAGCTACCTGAAAGCTCAGTTTGAAAAATTATCTCAACGTGATATGAAGAAGTACCTGGGAGAGATGAAGCGTCTGGCTGATGGGGGAGAAATGAATTTTGATGGCTATCTGGATAAGATGTCACATCTGGTGAATGAAGGAACGCTCGATCCTGATATTTTAAGCAAAATGCGAGATGCTGCACCACAATTAATTAGCTTCGCGAAGTCGTTTGACCCAAC  >  NC_000913/4260957‑4261227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A