Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,480,101	A→G	L124L (TTA→TTG)	yjgN →	conserved inner membrane protein YjgN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,480,101	0	A	G	100.0%	187.9 / NA	53	L124L (TTA→TTG)	yjgN	conserved inner membrane protein YjgN
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/29); total (24/29)

TTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTAC  >  NC_000913/4479966‑4480234

tttttttGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTtgatga                                                                                                                                   <  1:805775/140‑1 (MQ=255)

         ttttttCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCa                                                                                                                          <  1:341812/140‑1 (MQ=255)

               cTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCg                                                                                                                    <  1:747051/140‑1 (MQ=255)

                tATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGt                                                                                                                   <  2:757675/140‑1 (MQ=255)

                     cGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGAt                                                                                                              <  2:270363/140‑1 (MQ=255)

                     cGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGAt                                                                                                              <  2:81430/140‑1 (MQ=255)

                       cAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGAttt                                                                                                            >  1:231605/1‑140 (MQ=255)

                          tCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGt                                                                                                         <  1:777711/140‑1 (MQ=255)

                          tCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGt                                                                                                         <  1:490130/140‑1 (MQ=255)

                                    aGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGct                                                                                               <  2:231605/140‑1 (MQ=255)

                                    aGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGct                                                                                               >  1:362248/1‑140 (MQ=255)

                                      tgtCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCATAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGctct                                                                                             >  2:738198/1‑140 (MQ=255)

                                      tgtCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGctct                                                                                             >  1:685312/1‑140 (MQ=255)

                                      tgtCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGctct                                                                                             >  1:687494/1‑140 (MQ=255)

                                      tgtCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGctct                                                                                             >  1:698405/1‑140 (MQ=255)

                                      tgtCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGctct                                                                                             >  2:229096/1‑140 (MQ=255)

                                        tCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTAt                                                                                           >  1:525540/1‑140 (MQ=255)

                                            cagATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCATAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGaaa                                                                                       <  1:738198/140‑1 (MQ=255)

                                                tatGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGct                                                                                               >  1:864334/1‑128 (MQ=255)

                                                tatGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGct                                                                                               <  2:864334/128‑1 (MQ=255)

                                                 atGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGtt                                                                                  >  2:723247/1‑140 (MQ=255)

                                                  tGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTc                                                                                 >  2:490780/1‑140 (MQ=255)

                                                  tGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTc                                                                                 >  2:324733/1‑140 (MQ=255)

                                                    ccGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCtg                                                                               >  2:436630/1‑140 (MQ=255)

                                                         tgttgGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTggtggg                                                                          <  1:658875/140‑1 (MQ=255)

                                                         tgttgGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTggtggg                                                                          >  2:223402/1‑140 (MQ=255)

                                                                                tACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTa                                                   <  2:229307/140‑1 (MQ=255)

                                                                                 aCTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTaa                                                  <  2:599804/140‑1 (MQ=255)

                                                                                  cTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAAt                                                 <  1:638724/140‑1 (MQ=255)

                                                                                  cTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAAt                                                 <  1:229096/140‑1 (MQ=255)

                                                                                   tGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATg                                                >  2:697307/1‑140 (MQ=255)

                                                                                    gTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATgg                                               <  1:587977/140‑1 (MQ=255)

                                                                                       ggTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCa                                            <  1:490780/140‑1 (MQ=255)

                                                                                       ggTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCa                                            >  2:420666/1‑140 (MQ=255)

                                                                                        gTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCAt                                           >  1:278333/1‑140 (MQ=255)

                                                                                           ttGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTgg                                        >  1:309782/1‑140 (MQ=255)

                                                                                                  tatTTATGGCGGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTTGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATgggg                                 <  1:20387/140‑1 (MQ=255)

                                                                                                   atTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGa                                <  2:685312/140‑1 (MQ=255)

                                                                                                   atTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGa                                <  1:420666/140‑1 (MQ=255)

                                                                                                   atTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTCGCCGATTTTAATGGCCATTGGGATGGGGa                                <  1:497660/140‑1 (MQ=255)

                                                                                                     ttATGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACt                              <  1:723247/140‑1 (MQ=255)

                                                                                                        tGGCAGCAAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGtt                           <  2:687494/140‑1 (MQ=255)

                                                                                                             gcaAAAGGACTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCtt                      >  1:745454/1‑140 (MQ=255)

                                                                                                                     aCTGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGa              <  1:223402/140‑1 (MQ=255)

                                                                                                                       tGCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACa            <  1:579923/140‑1 (MQ=255)

                                                                                                                        gCGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACaa           >  2:657560/1‑140 (MQ=255)

                                                                                                                         cGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACaaa          >  1:462209/1‑140 (MQ=255)

                                                                                                                         cGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACaaa          >  1:408390/1‑140 (MQ=255)

                                                                                                                         cGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACa            <  2:379494/138‑1 (MQ=255)

                                                                                                                         cGTCATCAGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACa            >  1:379494/1‑138 (MQ=255)

                                                                                                                              tcaGGCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGc     <  1:200201/140‑1 (MQ=255)

                                                                                                                                 ggCTTTGATGACAAGTCTAAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTAc  <  2:881057/140‑1 (MQ=255)

                                                                                                                                 ggCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTAc  <  2:314823/140‑1 (MQ=255)

                                                                                                                                 ggCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTAc  <  2:189814/140‑1 (MQ=255)

                                                                                                                                 ggCCTTGATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTAc  >  1:149537/1‑140 (MQ=255)

TTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGGCCATTGGGATGGGGACTGTTTTCTTTATCTCGACAAAGATGCTAC  >  NC_000913/4479966‑4480234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A