Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,608,268	T→C	S28S (AGT→AGC)	rimI →	ribosomal‑protein‑S18‑alanine N‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,608,268	0	T	C	100.0%	164.8 / NA	47	S28S (AGT→AGC)	rimI	ribosomal‑protein‑S18‑alanine N‑acetyltransferase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/25); total (22/25)

ACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGA  >  NC_000913/4608135‑4608399

aCGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGaaaaa                                                                                                                               >  2:279363/1‑140 (MQ=255)

      aaaCCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGttt                                                                                                                         >  1:744551/1‑140 (MQ=255)

         cccAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTGCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGcc                                                                                                                      <  1:712688/140‑1 (MQ=255)

                gCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTTTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAAcc                                                                                                               <  2:85391/140‑1 (MQ=255)

                gCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAAcc                                                                                                               >  1:849936/1‑140 (MQ=255)

                  aCGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCATCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAg                                                                                                             >  2:95068/1‑140 (MQ=255)

                         gcgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGc                                                                                                      <  1:163855/140‑1 (MQ=255)

                         gcgTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGc                                                                                                      <  2:645936/140‑1 (MQ=255)

                                ggCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTAtct                                                                                               <  2:494155/140‑1 (MQ=255)

                                ggCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTAtct                                                                                               <  2:483388/140‑1 (MQ=255)

                                                  atGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCa                                                                             >  2:435249/1‑140 (MQ=255)

                                                          gATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCa                                                                     >  2:573224/1‑140 (MQ=255)

                                                          gATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCa                                                                     >  1:20648/1‑140 (MQ=255)

                                                            tttCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCaaa                                                                   >  1:639785/1‑140 (MQ=255)

                                                                  cccTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATggcg                                                             >  1:424673/1‑140 (MQ=255)

                                                                  cccTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATggcg                                                             <  1:814228/140‑1 (MQ=255)

                                                                  cccTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATggcg                                                             <  1:375428/140‑1 (MQ=255)

                                                                     tCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGgcggcg                                                          <  2:144651/140‑1 (MQ=255)

                                                                                 aTTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACg                                              >  2:363263/1‑140 (MQ=255)

                                                                                   ttACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCa                                            >  2:27469/1‑140 (MQ=255)

                                                                                     aCCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAg                                          <  1:363263/140‑1 (MQ=255)

                                                                                      ccGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAgt                                         <  1:95068/140‑1 (MQ=255)

                                                                                        ggcggcTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAgtgg                                       >  1:171045/1‑140 (MQ=255)

                                                                                          cggcTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAgtggtg                                     <  2:725132/140‑1 (MQ=255)

                                                                                           ggcTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGc                                    >  1:459067/1‑140 (MQ=255)

                                                                                            gcTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCt                                   >  1:800176/1‑140 (MQ=255)

                                                                                               tACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGa                                <  2:171045/140‑1 (MQ=255)

                                                                                               tACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGa                                <  2:800176/140‑1 (MQ=255)

                                                                                                 ccACATTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATg                              <  2:20648/140‑1 (MQ=255)

                                                                                                    caTTGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAg                           <  1:435249/140‑1 (MQ=255)

                                                                                                       tGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTa                        >  2:10537/1‑140 (MQ=255)

                                                                                                       tGAACAACGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTa                        <  2:639785/140‑1 (MQ=255)

                                                                                                         aacaacGCGCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACa                      >  2:859133/1‑140 (MQ=255)

                                                                                                              cgcgCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGa                                >  2:193447/1‑125 (MQ=255)

                                                                                                              cgcgCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGa                                <  1:193447/125‑1 (MQ=255)

                                                                                                              cgcgCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGtt                 >  1:564462/1‑140 (MQ=255)

                                                                                                              cgcgCCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGtt                 >  1:347677/1‑140 (MQ=255)

                                                                                                               gcgcCCACGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTc                >  2:753081/1‑140 (MQ=255)

                                                                                                                     aCGCCTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATAtt          <  2:744551/140‑1 (MQ=255)

                                                                                                                         cTTTCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCgg      <  2:499262/140‑1 (MQ=255)

                                                                                                                          tttCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGt     <  2:424673/140‑1 (MQ=255)

                                                                                                                          tttCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGt     <  2:347677/140‑1 (MQ=255)

                                                                                                                            tCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCg   >  2:736619/1‑140 (MQ=255)

                                                                                                                            tCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCg   >  2:793140/1‑140 (MQ=255)

                                                                                                                            tCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCg   <  1:573224/140‑1 (MQ=255)

                                                                                                                            tCCGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCg   <  1:279363/140‑1 (MQ=255)

                                                                                                                             ccGTGGAGCGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGa  <  1:10537/140‑1 (MQ=255)

ACGGGCAAACCCAACGGCACGCGCCGCGTTATGGCAACAAATTTGCACATATGAACACGATTTCTTCCCTCGAAACGACTGATTTACCGGCGGCTTACCACATTGAACAACGCGCCCACGCCTTTCCGTGGAGTGAAAAAACGTTTGCCAGCAACCAGGGCGAGCGTTATCTCAACTTTCAGTTAACGCAAAACGGCAAAATGGCGGCGTTTGCGATTACGCAAGTGGTGCTGGATGAAGCTACATTGTTCAATATTGCGGTCGA  >  NC_000913/4608135‑4608399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A