Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,609,828	A→G	T139A (ACC→GCC)	prfC →	peptide chain release factor RF3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,609,828	0	A	G	100.0%	189.6 / NA	54	T139A (ACC→GCC)	prfC	peptide chain release factor RF3
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (32/22); total (32/22)

ACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTAC  >  NC_000913/4609694‑4609962

aCTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCttt                                                                                                                                   <  1:367418/140‑1 (MQ=255)

  ttCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTAt                                                                                                                                 >  1:839374/1‑140 (MQ=255)

            aTACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACtt                                                                                                                       >  2:452457/1‑140 (MQ=255)

              aCCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGa                                                                                                                     >  2:427906/1‑140 (MQ=255)

                        cccTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATc                                                                                                           >  2:602151/1‑140 (MQ=255)

                          cTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCcg                                                                                                         <  1:626055/140‑1 (MQ=255)

                             aCGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGa                                                                                                      >  1:114244/1‑140 (MQ=255)

                             aCGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGa                                                                                                      <  2:522362/140‑1 (MQ=255)

                             aCGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGa                                                                                                      >  2:182261/1‑140 (MQ=255)

                                gcggTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGGCCGTGATATCCGCGAccc                                                                                                   >  2:385030/1‑140 (MQ=255)

                                       aCTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAg                                                                                            <  2:473000/140‑1 (MQ=255)

                                          gctgcCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAgctg                                                                                         <  1:182261/140‑1 (MQ=255)

                                                tGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGGCCGTGATATCCGCGACCCGATGGAGCTGCTCGAt                                                                                   <  1:385030/140‑1 (MQ=255)

                                                tGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGAt                                                                                   <  1:557735/140‑1 (MQ=255)

                                                          cGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTgaga                                                                         <  2:114244/140‑1 (MQ=255)

                                                          cGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTgaga                                                                         <  1:602151/140‑1 (MQ=255)

                                                            aCGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAAc                                                                       >  1:158852/1‑140 (MQ=255)

                                                               ccgcAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAg                                                                    >  2:104508/1‑140 (MQ=255)

                                                                cgcAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGGCCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGc                                                                   >  2:748046/1‑140 (MQ=255)

                                                                cgcAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGc                                                                   >  2:697299/1‑140 (MQ=255)

                                                                cgcAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGc                                                                   >  2:418793/1‑140 (MQ=255)

                                                                cgcAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGc                                                                   >  1:122363/1‑140 (MQ=255)

                                                                cgcAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGc                                                                   >  2:532123/1‑140 (MQ=255)

                                                                 gcAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCt                                                                  >  1:196601/1‑140 (MQ=255)

                                                                           ttGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGc                                                        >  2:879312/1‑140 (MQ=255)

                                                                                gATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTgc                                                   <  1:53111/140‑1 (MQ=255)

                                                                                 aTCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCgg                                                         >  2:587247/1‑133 (MQ=255)

                                                                                 aTCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCgg                                                         <  1:587247/133‑1 (MQ=255)

                                                                                  tCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTgcgc                                                 >  2:192565/1‑140 (MQ=255)

                                                                                   cGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGcc                                                >  1:639489/1‑140 (MQ=255)

                                                                                   cGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGcc                                                >  2:119930/1‑140 (MQ=255)

                                                                                             aGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTgg                                      >  1:644478/1‑140 (MQ=255)

                                                                                              gCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGc                                     >  1:70936/1‑140 (MQ=255)

                                                                                                 gATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGa                                  <  1:119930/140‑1 (MQ=255)

                                                                                                  aTGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGATAATCGGCTGTGCGCCGATCACCTGGCCGAt                                 >  2:543682/1‑140 (MQ=255)

                                                                                                       aGTTACCCGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCt                            <  2:70936/140‑1 (MQ=255)

                                                                                                            cccGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTgg                              <  2:34388/133‑1 (MQ=255)

                                                                                                            cccGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTgg                              >  1:34388/1‑133 (MQ=255)

                                                                                                            cccGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGc                       >  1:27019/1‑140 (MQ=255)

                                                                                                            cccGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGc                       <  1:418793/140‑1 (MQ=255)

                                                                                                             ccGTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCTGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCa                      <  2:305391/140‑1 (MQ=255)

                                                                                                               gTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAg                    <  1:532123/140‑1 (MQ=255)

                                                                                                               gTCTGCGCGACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAg                    <  2:97073/140‑1 (MQ=255)

                                                                                                                   gcgcGACACGCTGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGt                >  1:36731/1‑140 (MQ=255)

                                                                                                                    cgcgACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGGGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGtt               >  2:302312/1‑140 (MQ=255)

                                                                                                                    cgcgACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGtt               >  2:222854/1‑140 (MQ=255)

                                                                                                                    cgcgACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGtt               >  2:748136/1‑140 (MQ=255)

                                                                                                                       gACACGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTaa            <  1:697299/140‑1 (MQ=255)

                                                                                                                         cacGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAg          >  2:541682/1‑140 (MQ=255)

                                                                                                                         cacGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAg          >  1:294660/1‑140 (MQ=255)

                                                                                                                         cacGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAg          <  1:192565/140‑1 (MQ=255)

                                                                                                                          acGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAgg         <  2:182573/140‑1 (MQ=255)

                                                                                                                          acGCCGATCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAgg         >  1:182573/1‑140 (MQ=255)

                                                                                                                                 tCCTCGCCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTac  <  1:104508/140‑1 (MQ=255)

ACTTCTCGGAAGATACCTATCGTACCCTGACGGCGGTGGACTGCTGCCTGATGGTTATCGACGCCGCAAAAGGTGTTGAAGATCGTACCCGTAAGCTGATGGAAGTTACCCGTCTGCGCGACACGCCGATCCTCACCTTTATGAACAAACTTGACCGTGATATCCGCGACCCGATGGAGCTGCTCGATGAAGTTGAGAACGAGCTGAAAATCGGCTGTGCGCCGATCACCTGGCCGATTGGCTGCGGCAAGCTGTTTAAAGGCGTTTAC  >  NC_000913/4609694‑4609962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A