Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	227	52.0	1764248	99.3%	1751898	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,309	A→G	M361V (ATG→GTG)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,309	0	A	G	100.0%	133.9 / NA	38	M361V (ATG→GTG)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/18); total (20/18)

GTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAA  >  NC_000913/4619172‑4619443

gTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATGCCGAAGGTTTTGACAGTGAAATGGATACCCGCGCGCTGGGGGTg                                                                                                                                      <  2:255493/140‑1 (MQ=255)

  aTGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGc                                                                                                                                    <  2:461694/140‑1 (MQ=255)

      tAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTg                                                                                                                                <  1:72534/140‑1 (MQ=255)

        gCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGt                                                                                                                              >  1:459804/1‑140 (MQ=255)

            cacaAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCa                                                                                                                          <  2:721716/140‑1 (MQ=255)

              caAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTgg                                                                                                                                     >  2:453765/1‑127 (MQ=255)

              caAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTgg                                                                                                                                     <  1:453765/127‑1 (MQ=255)

                 aaaGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATggg                                                                                                                     <  1:402703/140‑1 (MQ=255)

                          accgacTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACg                                                                                                            >  2:347833/1‑140 (MQ=255)

                               cTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTc                                                                                                       >  2:493744/1‑140 (MQ=255)

                                  cGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAgg                                                                                                    <  2:431867/140‑1 (MQ=255)

                                         aaCTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGa                                                                                             >  2:71373/1‑140 (MQ=255)

                                         aaCTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGa                                                                                             <  1:314365/140‑1 (MQ=255)

                                         aaCTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGa                                                                                             <  1:211367/140‑1 (MQ=255)

                                           cTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGaca                                                                                           <  1:581373/140‑1 (MQ=255)

                                            tACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGacac                                                                                          >  1:271195/1‑140 (MQ=255)

                                             aCGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACAcc                                                                                         <  2:746839/140‑1 (MQ=255)

                                              cgcgAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCa                                                                                        >  1:710663/1‑140 (MQ=255)

                                                              aCAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCgg                                                                        <  2:710663/140‑1 (MQ=255)

                                                                aGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTc                                                                          <  2:137900/136‑1 (MQ=255)

                                                                aGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTc                                                                          >  1:137900/1‑136 (MQ=255)

                                                                aGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCt                                                                      >  1:514274/1‑140 (MQ=255)

                                                                  cGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCttt                                                                    >  2:183373/1‑140 (MQ=255)

                                                                     tGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACt                                                                 >  1:167080/1‑140 (MQ=255)

                                                                       cTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGa                                                               <  2:167080/140‑1 (MQ=255)

                                                                               aGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATgg                                                          >  1:862058/1‑137 (MQ=255)

                                                                               aGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATgg                                                          <  2:862058/137‑1 (MQ=255)

                                                                                    tCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTg                                                  <  2:271195/140‑1 (MQ=255)

                                                                                          cTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGAc                                            <  1:411226/140‑1 (MQ=255)

                                                                                              tACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAgg                                        >  1:282013/1‑140 (MQ=255)

                                                                                              tACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAgg                                        >  2:748962/1‑140 (MQ=255)

                                                                                              tACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAgg                                        >  2:809406/1‑140 (MQ=255)

                                                                                                  gAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGa                                    >  2:471363/1‑140 (MQ=255)

                                                                                                  gAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGa                                    >  1:557609/1‑140 (MQ=255)

                                                                                                          tGTCAGTGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGc                            >  1:241326/1‑140 (MQ=255)

                                                                                                                tGAAATGGATACCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGTCACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGc                      >  2:559511/1‑140 (MQ=255)

                                                                                                                          aCCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCgg                >  1:580966/1‑136 (MQ=255)

                                                                                                                          aCCCGCGCGCTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCgg                <  2:580966/136‑1 (MQ=255)

                                                                                                                                 cgcTGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTG‑TATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGa    >  1:490172/1‑140 (MQ=255)

                                                                                                                                    tGGGGGTGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGaaa  <  2:514274/140‑1 (MQ=255)

GTATGGTAGCGGCACAAAAAGGCCCGACCGACTTCGTTGAGAACTACGCGAAGTATCTGCCGACAGCGATGCTGACGAAAGCAGTCTATGCTGATACCGAAGGTTTTGTCAGTGAAATGGATACCCGCGCGCTGGGGATGGCAGTGGTTGCAATGGGCGGCGGACGCCGTCAGGCATCTGACACCATCGATTACAGCGTCGGCTTTACTGATATGGCGCGTCTGGGCGACCAGGTAGACGGTCAGCGTCCGCTGGCGGTTATCCACGCGAAA  >  NC_000913/4619172‑4619443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A