Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F73 I1 R1	21	115.3	3996176	99.3%	3968202	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,748,966	G→C	V130V (GTG→GTC)	sgbH →	3‑keto‑L‑gulonate‑6‑phosphate decarboxylase SgbH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,748,966	0	G	C	100.0%	313.0 / NA	83	V130V (GTG→GTC)	sgbH	3‑keto‑L‑gulonate‑6‑phosphate decarboxylase SgbH
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (40/43); total (40/43)

CCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGGGATTAC  >  NC_000913/3748830‑3749094

ccATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCgg                                                                                                                               <  1:1804089/140‑1 (MQ=255)

  atcatcTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCa                                                                                                                             <  2:637503/140‑1 (MQ=255)

  atcatcTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCa                                                                                                                             <  1:1857991/140‑1 (MQ=255)

  atcatcTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCa                                                                                                                             <  2:1075745/140‑1 (MQ=255)

  atcatcTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCa                                                                                                                             <  2:1523531/140‑1 (MQ=255)

  atcatcTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCa                                                                                                                             <  2:1967000/140‑1 (MQ=255)

   tcatcTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAg                                                                                                                            >  2:460/1‑140 (MQ=255)

    catcTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAgg                                                                                                                           >  2:215492/1‑140 (MQ=255)

    catcTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAgg                                                                                                                           <  1:1158337/140‑1 (MQ=255)

    catcTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAgg                                                                                                                           <  1:460/140‑1 (MQ=255)

    catcTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAgg                                                                                                                           <  1:20164/140‑1 (MQ=255)

     atcTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGc                                                                                                                          >  1:398472/1‑140 (MQ=255)

     atcTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGc                                                                                                                          <  1:275780/140‑1 (MQ=255)

          cgccgcgccgCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCa                                                                                                                             >  2:416376/1‑132 (MQ=255)

          cgccgcgccgCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCa                                                                                                                             <  1:416376/132‑1 (MQ=255)

          cgccgcgccgCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCAttt                                                                                                                     >  1:1586989/1‑140 (MQ=255)

           gccgcgccgcTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACAGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCa                                                                                                                        <  2:1606389/136‑1 (MQ=255)

           gccgcgccgcTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACAGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCa                                                                                                                        >  1:1606389/1‑136 (MQ=255)

               cgccgcTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTatcat                                                                                                                >  1:88667/1‑140 (MQ=255)

               cgccgcTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTatcat                                                                                                                <  1:1146253/140‑1 (MQ=255)

                 ccgcTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATcg                                                                                                              <  1:1893602/140‑1 (MQ=255)

                         gACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTg                                                                                                      <  1:368712/140‑1 (MQ=255)

                            ggTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATg                                                                                                   >  2:375721/1‑140 (MQ=255)

                                     aGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCa                                                                                          <  2:88667/140‑1 (MQ=255)

                                        ccACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCg                                                                                       >  1:917481/1‑140 (MQ=255)

                                        ccACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCg                                                                                       >  2:483172/1‑140 (MQ=255)

                                        ccACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCg                                                                                       >  1:578115/1‑140 (MQ=255)

                                          aCGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCggg                                                                                     <  1:929704/140‑1 (MQ=255)

                                          aCGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCggg                                                                                     >  2:565964/1‑140 (MQ=255)

                                           cGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGGCGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGc                                                                                    >  1:152016/1‑140 (MQ=255)

                                            gCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCa                                                                                   >  1:579495/1‑140 (MQ=255)

                                             cAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCaa                                                                                  <  1:1296242/140‑1 (MQ=255)

                                                     caACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTgggg                                                                          >  1:182428/1‑140 (MQ=255)

                                                      aaCGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGGCGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGc                                                                         >  1:1710989/1‑140 (MQ=255)

                                                          cTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCggg                                                                                     >  2:240758/1‑124 (MQ=255)

                                                          cTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCggg                                                                                     <  1:372645/124‑1 (MQ=255)

                                                          cTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCggg                                                                                     >  2:372645/1‑124 (MQ=255)

                                                          cTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCggg                                                                                     <  1:240758/124‑1 (MQ=255)

                                                          cTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAg                                                                     <  2:1145796/140‑1 (MQ=255)

                                                           tGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGc                                                                    >  1:1883934/1‑140 (MQ=255)

                                                           tGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGc                                                                    <  1:375721/140‑1 (MQ=255)

                                                             cGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCg                                                                  >  1:1698774/1‑140 (MQ=255)

                                                                  gTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGt                                                                              >  2:1018933/1‑123 (MQ=255)

                                                                  gTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGt                                                                              <  1:1018933/123‑1 (MQ=255)

                                                                     aaaTTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCa                                                          >  1:747009/1‑140 (MQ=255)

                                                                       aTTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACCACGCCCTCGACTGGCATCGTAATGGCGTCCGGCAGGCCATTTTTCATCGCGGTCGGGAAGCCCAGGACCGCCGGCGAAACTGGGGCGAAGCCGATCTggggcg                                                        >  2:1908978/1‑136 (MQ=255)

                                                                          cAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCAt                                                     >  2:422529/1‑140 (MQ=255)

                                                                          cAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCAt                                                     >  1:1192812/1‑140 (MQ=255)

                                                                           aGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATg                                                    <  1:422529/140‑1 (MQ=255)

                                                                              tAGAGCTGTTCGGTAACTGGACGCTGGCCGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAg                                                 <  1:565964/140‑1 (MQ=255)

                                                                               agagCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAgg                                                >  2:417348/1‑140 (MQ=255)

                                                                                gagCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGgc                                               <  2:264921/140‑1 (MQ=255)

                                                                                gagCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGgc                                               >  1:1530626/1‑140 (MQ=255)

                                                                                 agCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGgcg                                              >  2:276827/1‑140 (MQ=255)

                                                                                 agCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGgcg                                              <  1:1330527/140‑1 (MQ=255)

                                                                                 agCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGgcg                                              <  2:917481/140‑1 (MQ=255)

                                                                                 agCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGgcg                                              <  2:1530626/140‑1 (MQ=255)

                                                                                 agCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGgcg                                              >  2:1330527/1‑140 (MQ=255)

                                                                                     gTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCttt                                          <  2:1698774/140‑1 (MQ=255)

                                                                                     gTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCttt                                          >  2:627851/1‑140 (MQ=255)

                                                                                       tCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCa                                        >  2:600000/1‑140 (MQ=255)

                                                                                       tCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCa                                        >  1:878028/1‑140 (MQ=255)

                                                                                           tAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGata                                    >  2:1233125/1‑140 (MQ=255)

                                                                                           tAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGata                                    >  1:288704/1‑140 (MQ=255)

                                                                                            aaCTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGatat                                   <  1:276827/140‑1 (MQ=255)

                                                                                                ggacgctggacgACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGT‑GGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGcc                              >  2:27447/1‑140 (MQ=255)

                                                                                                   cgctggacgACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCtt                            >  2:418981/1‑140 (MQ=255)

                                                                                                   cgctggacgACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCtt                            <  1:215492/140‑1 (MQ=255)

                                                                                                   cgctggacgACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCtt                            >  1:173099/1‑140 (MQ=255)

                                                                                                      tggacgACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCTTGAg                         <  2:182428/140‑1 (MQ=255)

                                                                                                      tggacgACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCTTGAg                         <  1:417348/140‑1 (MQ=255)

                                                                                                      tggacgACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCTTGAg                         <  2:578115/140‑1 (MQ=255)

                                                                                                      tggacgACGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCTTGAg                         <  2:780103/140‑1 (MQ=255)

                                                                                                        gacgacGCCCGCGACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCTTGAGCt                       >  1:907685/1‑140 (MQ=255)

                                                                                                                   cgACTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGT‑GGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTgg           <  1:27447/140‑1 (MQ=255)

                                                                                                                     aCTGGCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGc          <  2:152016/140‑1 (MQ=255)

                                                                                                                        ggCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCggg       <  1:529347/140‑1 (MQ=255)

                                                                                                                        ggCATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCggg       >  2:301783/1‑140 (MQ=255)

                                                                                                                          cATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGGGAt     >  2:309709/1‑140 (MQ=255)

                                                                                                                          cATCGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGGGAt     <  2:907685/140‑1 (MQ=255)

                                                                                                                             cGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGGGATTAc  <  2:747009/140‑1 (MQ=255)

                                                                                                                             cGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGGGATTAc  <  1:301783/140‑1 (MQ=255)

                                                                                                                             cGTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGGGATTAc  <  1:627851/140‑1 (MQ=255)

                                                                                                                              gTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTgg           >  2:838102/1‑130 (MQ=255)

                                                                                                                              gTATTGGCGTCCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTgg           <  1:838102/130‑1 (MQ=255)

CCATCATCTGCGCCGCGCCGCTCGCGACGGTAGAAAAAGGCCACGCAATGGCACAACGCTGCGGGGGTGAAATTCAGATAGAGCTGTTCGGTAACTGGACGCTGGACGACGCCCGCGACTGGCATCGTATTGGCGTGCGGCAGGCCATTTATCATCGCGGTCGTGATGCACAGGCCAGCGGGCAACAGTGGGGCGAAGCCGATCTGGCACGCATGAAGGCGCTTTCAGATATCGGCCTTGAGCTTTCCATTACTGGCGGGATTAC  >  NC_000913/3748830‑3749094

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A