Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F73 I1 R1	21	115.3	3996176	99.3%	3968202	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,140,585	G→T	S546* (TCG→TAG)	ptsA ←	putative PTS multiphosphoryl transfer protein PtsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,140,585	0	G	T	100.0%	630.4 / NA	173	S546* (TCG→TAG)	ptsA	putative PTS multiphosphoryl transfer protein PtsA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (92/81); total (92/81)

Only 100 of 174 total aligned reads displayed.

GGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCA  >  NC_000913/4140450‑4140720

ggATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACgg                                                                                                                                     <  1:868765/140‑1 (MQ=255)

ggATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACgg                                                                                                                                     <  1:1565351/140‑1 (MQ=255)

  aTTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCa                                                                                                                                   <  2:209283/140‑1 (MQ=255)

  aTTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCa                                                                                                                                   <  2:1912410/140‑1 (MQ=255)

      aGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTc                                                                                                                               <  1:1307768/140‑1 (MQ=255)

            ttgtAGTGACGAGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCat                                                                                                                         >  2:1839886/1‑140 (MQ=255)

             tgtAGTGACGAGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCatg                                                                                                                        >  2:773097/1‑140 (MQ=255)

                aGTGACGAGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCatgatg                                                                                                                     <  1:773097/140‑1 (MQ=255)

                  tGACGAGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGcc                                                                                                                   >  1:248079/1‑140 (MQ=255)

                    aCGAGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGa                                                                                                                 >  2:26944/1‑140 (MQ=255)

                       aGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCt                                                                                                              >  1:721322/1‑140 (MQ=255)

                          aaCCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGa                                                                                                           <  2:707778/140‑1 (MQ=255)

                                aGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGAtttt                                                                                                       >  2:1269485/1‑138 (MQ=255)

                                aGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTtct                                                                                                     >  2:954956/1‑140 (MQ=255)

                                 gCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTtctc                                                                                                    >  1:1376600/1‑140 (MQ=255)

                                   gTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCtcat                                                                                                  <  1:276615/140‑1 (MQ=255)

                                   gTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCtcat                                                                                                  <  1:1502549/140‑1 (MQ=255)

                                      aTCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCaa                                                                                               >  2:1102831/1‑140 (MQ=255)

                                      aTCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCaa                                                                                               >  2:301818/1‑140 (MQ=255)

                                      aTCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCaa                                                                                               >  1:911093/1‑140 (MQ=255)

                                       tCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCaaa                                                                                              >  1:38499/1‑140 (MQ=255)

                                       tCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCaaa                                                                                              >  2:966086/1‑140 (MQ=255)

                                            aTCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGAtttt                                                                                                       >  1:1730557/1‑126 (MQ=255)

                                            aTCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGAtttt                                                                                                       <  2:1730557/126‑1 (MQ=255)

                                            aTCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGaa                                                                                         <  1:81603/140‑1 (MQ=255)

                                             tCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAAt                                                                                        <  2:1376600/140‑1 (MQ=255)

                                                       gcagATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTa                                                                              <  1:82079/140‑1 (MQ=255)

                                                       gcagATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTa                                                                              <  2:119037/140‑1 (MQ=255)

                                                       gcagATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTa                                                                              >  1:1050958/1‑140 (MQ=255)

                                                       gcagATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTa                                                                              >  1:563080/1‑140 (MQ=255)

                                                        cagATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTa                                                                              >  2:1586550/1‑139 (MQ=255)

                                                         agATACTGCGTCAGGTCATTACTACCAATAGTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACg                                                                            <  1:47404/140‑1 (MQ=255)

                                                         agATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACg                                                                            <  1:954956/140‑1 (MQ=255)

                                                         agATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACg                                                                            <  1:104134/140‑1 (MQ=255)

                                                           aTACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTa                                                                          >  1:1664507/1‑140 (MQ=255)

                                                                gCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGc                                                                     >  2:1033211/1‑140 (MQ=255)

                                                                 cGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTa                                                                              <  2:729250/130‑1 (MQ=255)

                                                                 cGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCt                                                                    >  1:646335/1‑140 (MQ=255)

                                                                  gTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTg                                                                   >  1:1867406/1‑140 (MQ=255)

                                                                   tCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGt                                                                  >  2:1529021/1‑140 (MQ=255)

                                                                     aGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGttt                                                                >  1:393732/1‑140 (MQ=255)

                                                                     aGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGttt                                                                >  1:1131177/1‑140 (MQ=255)

                                                                     aGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGttt                                                                >  2:217270/1‑140 (MQ=255)

                                                                     aGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGtt                                                                 >  2:295085/1‑139 (MQ=255)

                                                                       gTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTgg                                                              >  2:1363702/1‑140 (MQ=255)

                                                                         cATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCt                                                            >  2:1616051/1‑140 (MQ=255)

                                                                         cATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCt                                                            <  2:1521839/140‑1 (MQ=255)

                                                                           ttACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTc                                                          >  2:468834/1‑140 (MQ=255)

                                                                           ttACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTc                                                          >  2:1024441/1‑140 (MQ=255)

                                                                               tacCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGcc                                                      <  1:504299/140‑1 (MQ=255)

                                                                                 ccAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAg                                                    >  1:1945875/1‑140 (MQ=255)

                                                                                 ccAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAg                                                    >  1:188101/1‑140 (MQ=255)

                                                                                    aTACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTTATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGttt                                                 >  2:167504/1‑140 (MQ=255)

                                                                                    aTACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGttt                                                 >  1:1256143/1‑140 (MQ=255)

                                                                                      aCTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGc                                                       <  2:1435570/132‑1 (MQ=255)

                                                                                      aCTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGttttt                                               >  2:617986/1‑140 (MQ=255)

                                                                                        tAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTtttct                                             >  1:444022/1‑140 (MQ=255)

                                                                                         aaaGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTtttctt                                            >  1:1552791/1‑140 (MQ=255)

                                                                                            gAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCa                                         <  2:1664507/140‑1 (MQ=255)

                                                                                            gAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCa                                         <  2:900806/140‑1 (MQ=255)

                                                                                            gAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCa                                         <  1:258144/140‑1 (MQ=255)

                                                                                             aaaTCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCAc                                        <  2:493734/140‑1 (MQ=255)

                                                                                             aaaTCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCAc                                        >  1:1997639/1‑140 (MQ=255)

                                                                                              aatcaatcTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCAcc                                       <  1:1024441/140‑1 (MQ=255)

                                                                                               atcaatcTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCAccc                                      >  1:464152/1‑140 (MQ=255)

                                                                                                tcaatcTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTtttcttt                                           <  2:1911709/134‑1 (MQ=255)

                                                                                                tcaatcTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTtttcttt                                           >  1:1911709/1‑134 (MQ=255)

                                                                                                 caatcTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTTATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCAt                                    <  1:167504/140‑1 (MQ=255)

                                                                                                 caatcTCTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCAt                                    <  1:301818/140‑1 (MQ=255)

                                                                                                    tctcTTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAg                                 <  1:1839886/140‑1 (MQ=255)

                                                                                                      tctTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGAt                               <  1:980686/140‑1 (MQ=255)

                                                                                                       ctTCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGAtc                              >  1:103499/1‑140 (MQ=255)

                                                                                                         tCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCAt                                    <  2:1876619/132‑1 (MQ=255)

                                                                                                         tCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGAtctc                            <  2:444022/140‑1 (MQ=255)

                                                                                                         tCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGAtctc                            <  2:38499/140‑1 (MQ=255)

                                                                                                         tCGCAGCATTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGAtctc                            <  2:1050958/140‑1 (MQ=255)

                                                                                                           gcagcaTTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCtt                          <  2:1945875/140‑1 (MQ=255)

                                                                                                            cagcaTTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAg                                 >  2:249759/1‑132 (MQ=255)

                                                                                                            cagcaTTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTc                         <  1:1096441/140‑1 (MQ=255)

                                                                                                              gcaTTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCa                       <  2:1179690/140‑1 (MQ=255)

                                                                                                              gcaTTGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCa                       <  2:563080/140‑1 (MQ=255)

                                                                                                                  tGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCAt                                    <  1:1437911/123‑1 (MQ=255)

                                                                                                                  tGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTga                   >  2:629548/1‑140 (MQ=255)

                                                                                                                  tGATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTga                   >  2:1494564/1‑140 (MQ=255)

                                                                                                                   gATCGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGACTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTgag                  <  1:217270/140‑1 (MQ=255)

                                                                                                                      cGATGATGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTgaggag               <  1:360426/140‑1 (MQ=255)

                                                                                                                       gatgatGAACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGgaga              <  1:629548/140‑1 (MQ=255)

                                                                                                                         tgatgaACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGatc            >  1:1813983/1‑140 (MQ=255)

                                                                                                                          gatgaACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGatca           <  1:1834246/140‑1 (MQ=255)

                                                                                                                           atgaACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTgaggag               <  2:1844014/135‑1 (MQ=255)

                                                                                                                           atgaACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGatcat          >  2:1972389/1‑140 (MQ=255)

                                                                                                                             gaACATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCg        <  2:1813983/140‑1 (MQ=255)

                                                                                                                              aaCATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCgg       >  1:612058/1‑140 (MQ=255)

                                                                                                                                cATCACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCAtt                     <  1:725822/124‑1 (MQ=255)

                                                                                                                                   cACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTgagga                <  2:1308279/126‑1 (MQ=255)

                                                                                                                                   cACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTgagga                >  1:1308279/1‑126 (MQ=255)

                                                                                                                                   cACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGatcat          <  1:515789/132‑1 (MQ=255)

                                                                                                                                   cACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGatcat          >  2:515789/1‑132 (MQ=255)

                                                                                                                                   cACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCa  >  1:1524981/1‑140 (MQ=255)

                                                                                                                                   cACCTACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCa  >  1:948460/1‑140 (MQ=255)

GGATTCAGGCTGTTGTAGTGACGAGTAACCTTAGCGTTATCGCGATCCACCGCCAGCAGATACTGCGTCAGGTCATTACTACCAATACTAAAGAAATCAATCTCTTCGCAGCATTGATCGATGATGAACATCACCGACGGCACTTCCAGCATGATGCCGAGCTGGATTTTCTCATCAAACGGAATGTGTTCGTTACGTAGTTGCTGTTTGGCTTCCGCCAGTTTTTCTTTCACCCATAAGATCTCTTCCATTGAGGAGATCATCGGGATCA  >  NC_000913/4140450‑4140720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A