Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F73 I1 R1 30 83.3 2909128 99.3% 2888764 139.1

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,234,777 C→A V367L (GTG→TTG)  rlmG ← 23S rRNA m(2)G1835 methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,234,7770CA100.0% 164.5 / NA 46V367L (GTG→TTG) rlmG23S rRNA m(2)G1835 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/28);  total (18/28)

GCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTG  >  NC_000913/3234650‑3234906
                                                                                                                               |                                                                                                                                 
gCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTattatt                                                                                                                       <  2:1228913/140‑1 (MQ=255)
   tGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATtcgt                                                                                                                    <  1:78833/140‑1 (MQ=255)
      cgcgTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGc                                                                                                                 <  1:464284/140‑1 (MQ=255)
       gcgTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCa                                                                                                                >  2:821987/1‑140 (MQ=255)
        cgTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCaa                                                                                                               <  1:1071805/140‑1 (MQ=255)
        cgTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCaa                                                                                                               <  1:830291/140‑1 (MQ=255)
        cgTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCaa                                                                                                               <  1:461076/140‑1 (MQ=255)
                ccGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCTAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAgtggtg                                                                                                       <  2:1392811/140‑1 (MQ=255)
                  ggCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCa                                                                                                     <  1:387728/140‑1 (MQ=255)
                    ccTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGt                                                                                                   >  2:94958/1‑140 (MQ=255)
                     cTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGtt                                                                                                  <  1:821987/140‑1 (MQ=255)
                       ggAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGc                                                                                                >  1:1229746/1‑140 (MQ=255)
                          gTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTACACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGa                                                                                             <  1:1069794/140‑1 (MQ=255)
                                  cTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAAtcttc                                                                                     >  1:962988/1‑140 (MQ=255)
                                  cTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAAtcttc                                                                                     >  1:842290/1‑140 (MQ=255)
                                   tGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAAtcttct                                                                                    <  2:736293/140‑1 (MQ=255)
                                   tGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAAtcttct                                                                                    >  1:692789/1‑140 (MQ=255)
                                    gAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATcttctt                                                                                   <  2:892930/140‑1 (MQ=255)
                                    gAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATcttctt                                                                                   <  1:541666/140‑1 (MQ=255)
                                      aCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCa                                                                                 <  2:962988/140‑1 (MQ=255)
                                       cGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAg                                                                                >  1:507337/1‑140 (MQ=255)
                                          aGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGAGGTGCAGTTGCCGAAAATCTTCTTCAGttt                                                                             >  1:161102/1‑140 (MQ=255)
                                           ggCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTa                                                                            >  2:1357987/1‑140 (MQ=255)
                                            gCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTAt                                                                           <  2:1229746/140‑1 (MQ=255)
                                            gCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGAGGTGCAGTTGCCGAAAATCTTCTTCAGTTTAt                                                                           <  2:161102/140‑1 (MQ=255)
                                                 aTAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAg                                                                      >  2:1007568/1‑140 (MQ=255)
                                                          ttACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAgg                                                             >  2:1444946/1‑140 (MQ=255)
                                                                    tCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTATTCCAGGTGACGGTTgg                                                   <  2:919283/140‑1 (MQ=255)
                                                                    tCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTgg                                                   <  2:736117/140‑1 (MQ=255)
                                                                    tCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTgg                                                   <  2:1315232/140‑1 (MQ=255)
                                                                     ccGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGc                                                  <  1:586642/140‑1 (MQ=255)
                                                                      cGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCa                                                 <  1:238715/140‑1 (MQ=255)
                                                                      cGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCa                                                 <  2:749995/140‑1 (MQ=255)
                                                                      cGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCa                                                 <  1:853020/140‑1 (MQ=255)
                                                                      cGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCa                                                 <  1:1074418/140‑1 (MQ=255)
                                                                       ggCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCaa                                                <  2:1279417/140‑1 (MQ=255)
                                                                               aCTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATAc                                        >  2:1194817/1‑140 (MQ=255)
                                                                                cTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATACa                                       <  2:72750/140‑1 (MQ=255)
                                                                                  cccAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATACAGc                                     >  1:1442898/1‑140 (MQ=255)
                                                                                      aTCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATACAGCTCGc                                 >  2:951870/1‑140 (MQ=255)
                                                                                          tACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGtt                             <  2:842290/140‑1 (MQ=255)
                                                                                           aCCGACGACGTCCCAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTg                            >  1:1358411/1‑140 (MQ=255)
                                                                                                       ccAGCTTCACCGCTTTCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAg                >  2:639056/1‑140 (MQ=255)
                                                                                                                   cTTTGAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCgtgg    >  1:994288/1‑140 (MQ=255)
                                                                                                                    tttCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCgtggt   <  2:1442898/140‑1 (MQ=255)
                                                                                                                     ttCAGTACCAAAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCgtggtg  >  1:1104893/1‑140 (MQ=255)
                                                                                                                               |                                                                                                                                 
GCTTGCCGCGTCTTATCCGGCCTGGAGTAACGTGCTGAACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAGGTAACTCCCAATCTTACCGACGACGTCCCAGCTTCACCGCTTTCAGTACCACAAATTTATTATTCGTCGCAATAGTGGTGCAGTTGCCGAAAATCTTCTTCAGTTTATGGAAGTAATCCAGGTGACGGTTGGCAACGATATACAGCTCGCCGTTGATTTTCAGGCAGCGGCGGGCGTGGTG  >  NC_000913/3234650‑3234906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A