Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F73 I1 R1	30	83.3	2909128	99.3%	2888764	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,386,659	C→A	R510S (AGG→AGT)	aaeB ←	aromatic carboxylic acid efflux pump subunit AaeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,386,659	0	C	A	100.0%	199.4 / NA	57	R510S (AGG→AGT)	aaeB	aromatic carboxylic acid efflux pump subunit AaeB
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (26/31); total (26/31)

ACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTA  >  NC_000913/3386532‑3386792

aCTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGAt                                                                                                                           <  2:1343025/140‑1 (MQ=255)

aCTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGAt                                                                                                                           >  1:660469/1‑140 (MQ=255)

           aGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCAc                                                                                                                <  1:1165077/140‑1 (MQ=255)

           aGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCAc                                                                                                                >  2:1062548/1‑140 (MQ=255)

            gCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCAcc                                                                                                               >  1:460905/1‑140 (MQ=255)

              aaaaaCAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAg                                                                                                             <  1:1008185/140‑1 (MQ=255)

              aaaaaCAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAg                                                                                                             <  1:963637/140‑1 (MQ=255)

                      ctgctgATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATaa                                                                                                     <  2:773082/140‑1 (MQ=255)

                       tgctgATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAAc                                                                                                    >  1:333815/1‑140 (MQ=255)

                           gATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTg                                                                                                <  1:945798/140‑1 (MQ=255)

                                 gTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAAcgcg                                                                                          <  1:296478/140‑1 (MQ=255)

                                   gCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAg                                                                                        >  1:1324594/1‑140 (MQ=255)

                                    ccGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGc                                                                                       >  2:194725/1‑140 (MQ=255)

                                     cGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGca                                                                                      <  1:1378745/140‑1 (MQ=255)

                                      gggAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGcac                                                                                     >  2:1431380/1‑140 (MQ=255)

                                                    tttACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGAttt                                                                       >  1:243369/1‑140 (MQ=255)

                                                     ttACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTg                                                                      <  1:1431380/140‑1 (MQ=255)

                                                     ttACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTg                                                                      <  2:618276/140‑1 (MQ=255)

                                                       acgacgTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGcc                                                                    >  2:1276232/1‑140 (MQ=255)

                                                              gCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATgc                                                             >  2:189129/1‑140 (MQ=255)

                                                              gCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATgc                                                             >  2:34005/1‑140 (MQ=255)

                                                              gCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATgc                                                             >  2:1403529/1‑140 (MQ=255)

                                                              gCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATgc                                                             >  2:442090/1‑140 (MQ=255)

                                                              gCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATgc                                                             >  1:431543/1‑140 (MQ=255)

                                                              gCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATgc                                                             >  1:183138/1‑140 (MQ=255)

                                                                cacaTTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATgcgc                                                           <  1:1403529/140‑1 (MQ=255)

                                                                      ggTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCga                                                     >  1:1248061/1‑140 (MQ=255)

                                                                       gTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCgag                                                    >  2:321911/1‑140 (MQ=255)

                                                                            cATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAact                                               >  2:1237450/1‑140 (MQ=255)

                                                                             aTCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAactg                                              >  2:39246/1‑140 (MQ=255)

                                                                               cgcgGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAactgac                                            >  2:886369/1‑140 (MQ=255)

                                                                               cgcgGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAactgac                                            >  1:1291412/1‑140 (MQ=255)

                                                                               cgcgGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAactgac                                            >  1:1153577/1‑140 (MQ=255)

                                                                                gcgGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAactgact                                           >  1:526033/1‑140 (MQ=255)

                                                                                  ggAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAActgactga                                         <  2:360512/140‑1 (MQ=255)

                                                                                        aGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGa                                   <  1:1079486/140‑1 (MQ=255)

                                                                                              aGAAACAAACTGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCa                             <  1:892610/140‑1 (MQ=255)

                                                                                                        tGATTAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATc                   <  2:460905/140‑1 (MQ=255)

                                                                                                           ttAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAg                <  2:1356081/140‑1 (MQ=255)

                                                                                                           ttAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAg                <  1:39246/140‑1 (MQ=255)

                                                                                                           ttAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAg                <  1:886369/140‑1 (MQ=255)

                                                                                                            tAAGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGc               <  2:526033/140‑1 (MQ=255)

                                                                                                             aaGCAGTACACGTCCGGTACTGTCGTGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCggg                        <  2:944679/130‑1 (MQ=255)

                                                                                                             aaGCAGTACACGTCCGGTACTGTCGTGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCggg                        >  1:944679/1‑130 (MQ=255)

                                                                                                             aaGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCa              <  2:279686/140‑1 (MQ=255)

                                                                                                             aaGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCa              <  2:243369/140‑1 (MQ=255)

                                                                                                             aaGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCa              <  2:1156116/140‑1 (MQ=255)

                                                                                                             aaGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCa              <  2:183138/140‑1 (MQ=255)

                                                                                                              aGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCAc             <  2:104170/140‑1 (MQ=255)

                                                                                                              aGCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCAc             >  1:893997/1‑140 (MQ=255)

                                                                                                               gCAGTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACg            <  1:971772/140‑1 (MQ=255)

                                                                                                                  gTACACGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGata         <  2:1153577/140‑1 (MQ=255)

                                                                                                                    acacGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGataat       <  1:442090/140‑1 (MQ=255)

                                                                                                                    acacGTCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTG‑AATGGAAAGTCATCGGGTTATCCAGCACGataata      >  2:948472/1‑140 (MQ=255)

                                                                                                                         tCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTa  <  2:989265/140‑1 (MQ=255)

                                                                                                                         tCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTa  <  2:660469/140‑1 (MQ=255)

                                                                                                                         tCCGGTACTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTa  <  2:1324594/140‑1 (MQ=255)

ACTTATTCATCAGCAAAAACAGCTGCTGATAAAGTGCCGGGAGGTGGTTCTCTTTACGACGTGCCACATTGGTAGTCATCGCGGAAACAGCGGCAGAAACAAACTGATTAAGCAGTACACGTCCGGTCCTGTCGCGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTA  >  NC_000913/3386532‑3386792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A