Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A8 F73 I1 R1	30	83.3	2909128	99.3%	2888764	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,936,712	G→T	A145A (GCG→GCT)	rbsB →	ribose ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,936,712	0	G	T	100.0%	314.5 / NA	88	A145A (GCG→GCT)	rbsB	ribose ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (56/32); total (56/32)

GTGAAGATGGCTAACCAGGCGAACATCCCGGTTATCACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCGGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGAT  >  NC_000913/3936575‑3936847

gtgAAGATGGCTAACCAGGCGAACATCCCGGTTATCACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCtgg                                                                                                                                       >  1:360226/1‑140 (MQ=255)

 tgAAGATGGCTAACCAGGCGAACATCCCGGTTATCACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGt                                                                                                                                      >  2:328212/1‑140 (MQ=255)

 tgAAGATGGCTAACCAGGCGAACATCCCGGTTATCACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGt                                                                                                                                      >  1:588970/1‑140 (MQ=255)

 tgAAGATGGCTAACCAGGCGAACATCCCGGTTATCACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGt                                                                                                                                      >  2:1159459/1‑140 (MQ=255)

 tgAAGATGGCTAACCAGGCGAACATCCCGGTTATCACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGt                                                                                                                                      >  2:1308379/1‑140 (MQ=255)

 tgAAGATGGCTAACCAGGCGAACATCCCGGTTATCACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGt                                                                                                                                      >  1:1186482/1‑140 (MQ=255)

 tgAAGATGGCTAACCAGGCGAACATCCCGGTTATCACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGt                                                                                                                                      >  1:207574/1‑140 (MQ=255)

   aaGATGGCTAACCAGGCGAACATCCCGGTTATCACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGa                                                                                                                                    >  2:1433386/1‑140 (MQ=255)

        ggCTAACCAGGCGAACATCCCGGTTATCACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGATGAAAGCTGGTGAAGGTg                                                                                                                               >  2:204520/1‑140 (MQ=255)

        ggCTAACCAGGCGAACATCCCGGTTATCACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTg                                                                                                                               >  1:933925/1‑140 (MQ=255)

         gCTAACCAGGCGAACATCCCGGTTATCACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGc                                                                                                                              >  2:756159/1‑140 (MQ=255)

           tAACCAGGCGAACATCCCGGTTATCACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCa                                                                                                                            >  1:1215944/1‑140 (MQ=255)

                 ggCGAACATCCCGGTTATCACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTa                                                                                                                      <  2:1215944/140‑1 (MQ=255)

                         tCCCGGTTATCACTCTTGACCGCCAGGCAACGAAAGGTGAAGTG‑‑GAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCa                                                                                                            >  1:1029033/1‑140 (MQ=255)

                           ccGGTTATCACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCa                                                                                                            <  2:420089/140‑1 (MQ=255)

                              gTTATCACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAgg                                                                                                         <  2:1165410/140‑1 (MQ=255)

                                   cACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTg                                                                                                    <  1:738638/140‑1 (MQ=255)

                                   cACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTg                                                                                                    >  2:1419178/1‑140 (MQ=255)

                                    aCTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGc                                                                                                   >  1:1234080/1‑140 (MQ=255)

                                    aCTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAACCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGc                                                                                                   >  2:143620/1‑140 (MQ=255)

                                       ctTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTgg                                                                                                >  1:3460/1‑140 (MQ=255)

                                         tGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGc                                                                                                        >  1:973637/1‑130 (MQ=255)

                                         tGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGc                                                                                                        <  2:973637/130‑1 (MQ=255)

                                         tGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTa                                                                                              >  2:745088/1‑140 (MQ=255)

                                         tGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTa                                                                                              <  2:285690/140‑1 (MQ=255)

                                           aCCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTACTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACa                                                                                            <  2:436516/140‑1 (MQ=255)

                                           aCCGCCAGGCAACGAAAGGCGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACa                                                                                            >  2:1032417/1‑140 (MQ=255)

                                               ccAGGCAACGAAAGGTGAAGTGGTGAGCTCCATTGCTGCTGATAAGGTACTGGGGGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTGCATCCg                                                                                        <  2:899529/140‑1 (MQ=255)

                                               ccAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCg                                                                                        >  1:828511/1‑140 (MQ=255)

                                                cAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTg                                                                                                    <  2:73514/127‑1 (MQ=255)

                                                cAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTg                                                                                                    >  1:73514/1‑127 (MQ=255)

                                                cAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGc                                                                                       >  2:1107372/1‑140 (MQ=255)

                                                cAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGc                                                                                       >  1:272228/1‑140 (MQ=255)

                                                       cGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGt                                                                                <  2:1219329/140‑1 (MQ=255)

                                                        gAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTg                                                                               <  2:3460/140‑1 (MQ=255)

                                                          aaGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGaa                                                                             >  1:1357822/1‑140 (MQ=255)

                                                               gAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATAGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTgg                                                                        <  1:729724/140‑1 (MQ=255)

                                                                 aGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCg                                                                      >  1:755179/1‑140 (MQ=255)

                                                                 aGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCg                                                                      <  2:286274/140‑1 (MQ=255)

                                                                  gtggtgAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGCTTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGa                                                                     <  1:1419178/140‑1 (MQ=255)

                                                                       gAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCt                                                                <  2:1029033/140‑1 (MQ=255)

                                                                       gAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCt                                                                <  1:792327/140‑1 (MQ=255)

                                                                        aGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCtt                                                               >  2:654515/1‑140 (MQ=255)

                                                                             caTTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCcagc                                                          >  1:770399/1‑140 (MQ=255)

                                                                                    tCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGt                                                   >  2:97428/1‑140 (MQ=255)

                                                                                    tCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGt                                                   >  1:100024/1‑140 (MQ=255)

                                                                                        aTAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCt                                                                <  2:783756/123‑1 (MQ=255)

                                                                                        aTAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCt                                                                >  1:783756/1‑123 (MQ=255)

                                                                                            cGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTc                                           >  2:995811/1‑140 (MQ=255)

                                                                                            cGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTc                                           >  1:34979/1‑140 (MQ=255)

                                                                                             gTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTca                                          >  2:506950/1‑140 (MQ=255)

                                                                                             gTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTca                                          <  2:1234080/140‑1 (MQ=255)

                                                                                             gTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTca                                          <  1:519238/140‑1 (MQ=255)

                                                                                              tACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTcac                                         <  1:502613/140‑1 (MQ=255)

                                                                                               aCTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTcaca                                        >  1:1409143/1‑140 (MQ=255)

                                                                                                cTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACaa                                       <  1:756159/140‑1 (MQ=255)

                                                                                                cTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACaa                                       >  1:717369/1‑140 (MQ=255)

                                                                                                 tGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAg                                      >  2:125790/1‑140 (MQ=255)

                                                                                                 tGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAg                                      >  1:609727/1‑140 (MQ=255)

                                                                                                 tGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCCAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAg                                      >  2:177321/1‑140 (MQ=255)

                                                                                                  gggCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGt                                     >  2:306189/1‑140 (MQ=255)

                                                                                                  gggCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGt                                     >  2:857262/1‑140 (MQ=255)

                                                                                                  gggCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGACTTCCAGCAGGCCGTTGCTGCTCACAAGt                                     >  1:1180218/1‑140 (MQ=255)

                                                                                                  gcgcggcAAAATCGCTGGAGATTACATCGCGAAGAAAGCTGGTGAAGGAGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGt                                     >  2:1410830/3‑140 (MQ=255)

                                                                                                   ggcggcAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGtt                                    >  2:651509/1‑140 (MQ=255)

                                                                                                   ggcggcAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGtt                                    >  1:375827/1‑140 (MQ=255)

                                                                                                     cggcAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGTTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTa                                  >  2:611137/1‑140 (MQ=255)

                                                                                                     cggcAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTa                                  >  1:889991/1‑140 (MQ=255)

                                                                                                      ggcAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTaa                                 >  1:438804/1‑140 (MQ=255)

                                                                                                       gcAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAAt                                >  1:1072461/1‑140 (MQ=255)

                                                                                                       gcAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAAt                                >  1:991278/1‑140 (MQ=255)

                                                                                                        cAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTgctgct                                            <  2:358551/127‑1 (MQ=255)

                                                                                                        cAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTgctgct                                            >  1:358551/1‑127 (MQ=255)

                                                                                                        cAAAATCGCTGGTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATg                               <  1:745088/140‑1 (MQ=255)

                                                                                                                  ggTGATTACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTgccag                     <  2:991278/140‑1 (MQ=255)

                                                                                                                        tACATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTgccagccagcc               <  1:1032417/140‑1 (MQ=255)

                                                                                                                          cATCGCGAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGccagccagccag             <  1:328212/140‑1 (MQ=255)

                                                                                                                             cgcgAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCcagcag          >  1:316899/1‑140 (MQ=255)

                                                                                                                             cgcgAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCcagcag          >  2:1875/1‑140 (MQ=255)

                                                                                                                             cgcgAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCcagcag          >  1:910070/1‑140 (MQ=255)

                                                                                                                             cgcgAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCACAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCcagcag          >  1:484254/1‑140 (MQ=255)

                                                                                                                             agcgAAGAAAGCTGGCGAAGGCGCCAAAGTTATCGCGCTGCAAGGCATTGCTGGTACATCCGCAGCACGTGAACGTCGCGAAGGCTTCCAGCAGGCCGTTGCTGTTCACAAGTTTAATGTTCTCgccagccagccaaaag          >  2:355491/2‑136 (MQ=255)

                                                                                                                              gcgAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGa         >  1:1128027/1‑140 (MQ=255)

                                                                                                                               cgAAGAAAGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGAt        >  1:1175603/1‑140 (MQ=255)

                                                                                                                                    aaaGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGa   >  2:444238/1‑140 (MQ=255)

                                                                                                                                    aaaGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGa   <  2:408770/140‑1 (MQ=255)

                                                                                                                                     aaGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGAt  <  2:52784/140‑1 (MQ=255)

                                                                                                                                     aaGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGAt  <  1:306189/140‑1 (MQ=255)

                                                                                                                                     aaGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGAt  >  1:52784/1‑140 (MQ=255)

                                                                                                                                     aaGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGAt  <  1:599451/140‑1 (MQ=255)

                                                                                                                                     aaGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGAt  <  2:1409143/140‑1 (MQ=255)

                                                                                                                                     aaGCTGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGAt  <  2:1175603/140‑1 (MQ=255)

GTGAAGATGGCTAACCAGGCGAACATCCCGGTTATCACTCTTGACCGCCAGGCAACGAAAGGTGAAGTGGTGAGCCACATTGCTTCTGATAACGTACTGGGCGGCAAAATCGCTGGTGATTACATCGCGAAGAAAGCGGGTGAAGGTGCCAAAGTTATCGAGCTGCAAGGCATTGCTGGTACATCCGCAGCCCGTGAACGTGGCGAAGGCTTCCAGCAGGCCGTTGCTGCTCACAAGTTTAATGTTCTTGCCAGCCAGCCAGCAGATTTTGAT  >  NC_000913/3936575‑3936847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A