Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A16 F73 I1 R1 26 114.7 3982346 99.4% 3958451 139.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,435,412 C→A P69Q (CCG→CAG)  rsmB → 16S rRNA m(5)C967 methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,435,4120CA100.0% 403.2 / NA 113P69Q (CCG→CAG) rsmB16S rRNA m(5)C967 methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (68/45);  total (68/45)

Only 100 of 113 total aligned reads displayed.

ATCATTAAGCAACATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCCGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTCCGCAACTT  >  NC_000913/3435275‑3435548
                                                                                                                                         |                                                                                                                                        
aTCATTAAGCAACATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGa                                                                                                                                        >  1:225157/1‑140 (MQ=255)
      aaGCAACATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCg                                                                                                                                  >  1:253215/1‑140 (MQ=255)
      aaGCAACATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCg                                                                                                                                  >  1:145925/1‑140 (MQ=255)
        gCAACATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGc                                                                                                                                <  2:1191088/140‑1 (MQ=255)
        gCAACATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGc                                                                                                                                >  2:1140921/1‑140 (MQ=255)
            cATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAAc                                                                                                                            >  2:430269/1‑140 (MQ=255)
            cATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAAc                                                                                                                            >  1:707591/1‑140 (MQ=255)
            cATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAAc                                                                                                                            >  2:758022/1‑140 (MQ=255)
            cATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAAc                                                                                                                            >  2:1441399/1‑140 (MQ=255)
             aTTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACa                                                                                                                           >  2:307934/1‑140 (MQ=255)
               tCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGc                                                                                                                         <  1:675592/140‑1 (MQ=255)
                 tGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCgg                                                                                                                       >  1:410533/1‑140 (MQ=255)
                  gCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGa                                                                                                                      <  1:796410/140‑1 (MQ=255)
                   ccACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGTGGAc                                                                                                                     <  1:334262/140‑1 (MQ=255)
                   ccACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGAc                                                                                                                     <  1:924380/140‑1 (MQ=255)
                   ccACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGAc                                                                                                                     >  2:589031/1‑140 (MQ=255)
                     aCCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGATCGGCAAACAGCGGACCg                                                                                                                   <  1:1138582/140‑1 (MQ=255)
                      ccGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGt                                                                                                                  >  2:829843/1‑140 (MQ=255)
                      ccGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGt                                                                                                                  >  1:1731024/1‑140 (MQ=255)
                      ccGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGt                                                                                                                  >  1:1632400/1‑140 (MQ=255)
                       cGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTg                                                                                                                 >  1:1906980/1‑140 (MQ=255)
                         cTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCa                                                                                                               <  2:145206/140‑1 (MQ=255)
                         cTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCa                                                                                                               <  1:1895115/140‑1 (MQ=255)
                         cTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCa                                                                                                               >  1:1541437/1‑140 (MQ=255)
                         cTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCa                                                                                                               >  1:609585/1‑140 (MQ=255)
                         cTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCa                                                                                                               >  2:1176052/1‑140 (MQ=255)
                          tCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCAt                                                                                                              <  1:190976/140‑1 (MQ=255)
                             aGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCa                                                                                                               <  1:273795/136‑1 (MQ=255)
                             aGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCa                                                                                                               >  2:273795/1‑136 (MQ=255)
                             aGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTAc                                                                                                           >  1:1890565/1‑140 (MQ=255)
                             aGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTAc                                                                                                           <  2:1488606/140‑1 (MQ=255)
                                  aaaGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGAt                                                                                                      <  2:1906980/140‑1 (MQ=255)
                                  aaaGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGAt                                                                                                      <  1:430269/140‑1 (MQ=255)
                                   aaGTTTTCCAAAAAGGCAAAACAATTCTTTAAAAGTTGTGCCTTGGCGTACCGCCGTCGGCTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGAtt                                                                                                     <  2:1583295/140‑1 (MQ=255)
                                   aaGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGAtt                                                                                                     <  2:1549719/140‑1 (MQ=255)
                                       ttCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTAtgg                                                                                                 <  2:1310218/140‑1 (MQ=255)
                                        tCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTAtggt                                                                                                >  1:241738/1‑140 (MQ=255)
                                        tCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTAtggt                                                                                                <  2:145925/140‑1 (MQ=255)
                                         ccGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTAtggtt                                                                                               >  2:359206/1‑140 (MQ=255)
                                           gATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTAtggttgg                                                                                             <  2:1397958/140‑1 (MQ=255)
                                           gATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTAtggttgg                                                                                             <  2:609585/140‑1 (MQ=255)
                                              aaaGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGttt                                                                                          >  1:665188/1‑140 (MQ=255)
                                                aGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGt                                                                                        <  1:852841/140‑1 (MQ=255)
                                                     aaGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCaa                                                                                   <  2:782408/140‑1 (MQ=255)
                                                      aGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAAc                                                                                  <  1:1936824/140‑1 (MQ=255)
                                                      aGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAAc                                                                                  <  1:1986354/140‑1 (MQ=255)
                                                       gCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACt                                                                                 >  2:836640/1‑140 (MQ=255)
                                                       gCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACt                                                                                 <  1:379773/140‑1 (MQ=255)
                                                           ttcttcAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCtt                                                                             >  1:482242/1‑140 (MQ=255)
                                                            tcttcAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCttt                                                                            >  1:106020/1‑140 (MQ=255)
                                                            tcttcAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCttt                                                                            >  1:1424803/1‑140 (MQ=255)
                                                                cAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATAc                                                                        <  1:1295678/140‑1 (MQ=255)
                                                                  agagTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATAccc                                                                      <  1:758022/140‑1 (MQ=255)
                                                                   gagTTGTGCTTTGGCGTACTGCGTGCGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCg                                                                     <  1:1441399/140‑1 (MQ=255)
                                                                   gagTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCg                                                                     <  2:225157/140‑1 (MQ=255)
                                                                   gagTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCg                                                                     <  2:1731024/140‑1 (MQ=255)
                                                                       tgtgCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCAtt                                                                 <  1:829843/140‑1 (MQ=255)
                                                                       tgtgCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCAtt                                                                 <  2:1519466/140‑1 (MQ=255)
                                                                        gtgCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCAtt                                                                 <  1:845782/139‑1 (MQ=255)
                                                                        gtgCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCAtt                                                                 >  2:845782/1‑139 (MQ=255)
                                                                        gtgCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTc                                                                >  2:1326048/1‑140 (MQ=255)
                                                                        gtgCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTc                                                                >  1:1922015/1‑140 (MQ=255)
                                                                        gtgCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTc                                                                >  1:1849963/1‑140 (MQ=255)
                                                                        gtgCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTA‑GGTTGGTTTGTATCAACTGCTTTATACCCGCATTcc                                                               >  1:251544/1‑140 (MQ=255)
                                                                         tgCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTcc                                                               >  2:521485/1‑140 (MQ=255)
                                                                            tttGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCAcc                                                            <  1:307934/140‑1 (MQ=255)
                                                                              tGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTc                                                          >  1:1390207/1‑140 (MQ=255)
                                                                                gCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCAt                                                        >  2:438817/1‑140 (MQ=255)
                                                                                 cGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCAtg                                                       <  1:1140921/140‑1 (MQ=255)
                                                                                     cTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTA‑GGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTgcg                                                  <  2:251544/140‑1 (MQ=255)
                                                                                      tGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTgcg                                                  <  1:921278/140‑1 (MQ=255)
                                                                                        cGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATTGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCgct                                                >  2:1638479/1‑140 (MQ=255)
                                                                                        cGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCgct                                                >  2:1516675/1‑140 (MQ=255)
                                                                                        cGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCgct                                                >  2:1329375/1‑140 (MQ=255)
                                                                                        cGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCgct                                                >  1:275016/1‑140 (MQ=255)
                                                                                          tACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCgctgg                                              >  1:1151233/1‑140 (MQ=255)
                                                                                             gCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCgctggctg                                           <  1:1516675/140‑1 (MQ=255)
                                                                                                        tAGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGaa                                <  1:359206/140‑1 (MQ=255)
                                                                                                         aGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAg                               >  1:1844674/1‑140 (MQ=255)
                                                                                                         aGACTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAg                               >  2:1042511/1‑140 (MQ=255)
                                                                                                           aCTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGgc                             >  1:1073184/1‑140 (MQ=255)
                                                                                                            cTGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGgcg                            <  2:1180137/140‑1 (MQ=255)
                                                                                                             tGGCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGgcgc                           <  1:438817/140‑1 (MQ=255)
                                                                                                               gCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTa                         >  2:1952005/1‑140 (MQ=255)
                                                                                                               gCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTa                         >  2:71216/1‑140 (MQ=255)
                                                                                                               gCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTa                         >  1:760587/1‑140 (MQ=255)
                                                                                                               gCTGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCAGTACCTGATTATGGTTGGTTTGTATTAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTa                         >  2:1777589/1‑140 (MQ=255)
                                                                                                                 tGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATc                       <  1:589031/140‑1 (MQ=255)
                                                                                                                 tGATTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATc                       >  2:486598/1‑140 (MQ=255)
                                                                                                                   aTTAATAAGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGc                     >  2:873874/1‑140 (MQ=255)
                                                                                                                          aGTTAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAg              >  1:1774880/1‑140 (MQ=255)
                                                                                                                             tAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGt           >  2:1138657/1‑140 (MQ=255)
                                                                                                                             tAATGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGt           >  1:1227931/1‑140 (MQ=255)
                                                                                                                              aaTGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTc          >  2:1819132/1‑140 (MQ=255)
                                                                                                                               aTGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTcc         >  2:663233/1‑140 (MQ=255)
                                                                                                                               aTGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTcc         >  1:602125/1‑140 (MQ=255)
                                                                                                                               aTGGCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTcc         >  2:1914577/1‑140 (MQ=255)
                                                                                                                                  gCCCGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTCCGCa      >  2:1645495/1‑140 (MQ=255)
                                                                                                                                     cGTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTCCGCAACt   >  2:858774/1‑140 (MQ=255)
                                                                                                                                      gTCAGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTCCGCAACtt  >  2:1360976/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                        
ATCATTAAGCAACATTCTGCCACCGCTCCAGCAAAAAGTTTCCGATAAAGACAAAGCACTTCTTCAAGAGTTGTGCTTTGGCGTACTGCGTACGCTTTCGCAGTTAGACTGGCTGATTAATAAGTTAATGGCCCGTCCGATGACCGGCAAACAGCGGACCGTGCATTACCTGATTATGGTTGGTTTGTATCAACTGCTTTATACCCGCATTCCACCTCATGCTGCGCTGGCTGAAACGGTTGAAGGCGCTATCGCAATTAAGCGTCCGCAACTT  >  NC_000913/3435275‑3435548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A