Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F73 I1 R1	37	152.5	5346736	99.2%	5303962	139.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,771,784	C→A	intergenic (‑40/‑497)	yibI ← / → mtlA	DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,771,784	0	C	A	99.2%	866.7 / ‑0.5	242	intergenic (‑40/‑497)	yibI/mtlA	DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
Reads supporting (aligned to +/- strand): ref base C (0/2); new base A (115/125); total (115/127)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 246 total aligned reads displayed.

AATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACAT  >  NC_000913/3771649‑3771920

aaTCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGc                                                                                                                                      <  1:1962536/140‑1 (MQ=255)

aaTCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGc                                                                                                                                      <  2:1458170/140‑1 (MQ=255)

           ggATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAggg                                                                                                                           >  1:2535956/1‑140 (MQ=255)

             aTGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAg                                                                                                                         >  1:134385/1‑140 (MQ=255)

              tgtgATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGGAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGTATGGCAGCCAGAAGGGAGt                                                                                                                        >  2:2174103/1‑140 (MQ=255)

              tGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGt                                                                                                                        >  2:235869/1‑140 (MQ=255)

              tGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGt                                                                                                                        >  2:1146697/1‑140 (MQ=255)

              tGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGt                                                                                                                        >  1:2324620/1‑140 (MQ=255)

              tGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGt                                                                                                                        <  2:162940/140‑1 (MQ=255)

               gTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTc                                                                                                                       >  1:966620/1‑140 (MQ=255)

               gTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTc                                                                                                                       >  2:176567/1‑140 (MQ=255)

               gTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTc                                                                                                                       >  2:157075/1‑140 (MQ=255)

                tCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATgg                                                                                                                                       >  2:1220752/1‑123 (MQ=255)

                tCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGt                                                                                                                        <  2:118588/138‑1 (MQ=255)

                tCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCa                                                                                                                      >  2:650301/1‑140 (MQ=255)

                tCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCa                                                                                                                      >  2:1112816/1‑140 (MQ=255)

                tCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCa                                                                                                                      >  1:452638/1‑140 (MQ=255)

                tCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCa                                                                                                                      >  1:671189/1‑140 (MQ=255)

                tCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCa                                                                                                                      >  2:1798522/1‑140 (MQ=255)

                tCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCa                                                                                                                      >  2:1391327/1‑140 (MQ=255)

                tCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCa                                                                                                                      >  2:1496642/1‑140 (MQ=255)

                tCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCa                                                                                                                      >  2:2510130/1‑140 (MQ=255)

                tCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGATTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCa                                                                                                                      >  2:682580/1‑140 (MQ=255)

                     tATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTg                                                                                                                 >  1:391054/1‑140 (MQ=255)

                     tATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTg                                                                                                                 >  1:1079889/1‑140 (MQ=255)

                          cgATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGaa                                                                                                                              >  1:1298963/1‑122 (MQ=255)

                          cgATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGat                                                                                                               <  1:1837398/137‑1 (MQ=255)

                          cgATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGat                                                                                                               >  2:1837398/1‑137 (MQ=255)

                          cgATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATAtt                                                                                                            >  1:1790796/1‑140 (MQ=255)

                          cgATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATAtt                                                                                                            >  1:1648400/1‑140 (MQ=255)

                          cgATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATAtt                                                                                                            >  2:916591/1‑140 (MQ=255)

                            atgatTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATAtttt                                                                                                          >  1:738095/1‑140 (MQ=255)

                            atgatTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATAtttt                                                                                                          >  2:2578573/1‑140 (MQ=255)

                               atTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGAc                                                                                                       >  2:1054140/1‑140 (MQ=255)

                                        aaaaTCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCg                                                                                              <  1:551841/140‑1 (MQ=255)

                                             cACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTc                                                                                         >  1:1268833/1‑140 (MQ=255)

                                                     aGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATtct                                                                                 >  1:345818/1‑140 (MQ=255)

                                                     aGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATtct                                                                                 <  1:1391327/140‑1 (MQ=255)

                                                     aGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATtct                                                                                 <  1:819008/140‑1 (MQ=255)

                                                        cGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGc                                                                              >  1:1187207/1‑140 (MQ=255)

                                                          aaGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCa                                                                            >  1:1447528/1‑140 (MQ=255)

                                                           aGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCAt                                                                           <  2:2063634/140‑1 (MQ=255)

                                                             aTCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATaa                                                                         >  2:1489987/1‑140 (MQ=255)

                                                             aTCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATaa                                                                         >  2:2241052/1‑140 (MQ=255)

                                                                aGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAacac                                                                      >  2:1983651/1‑140 (MQ=255)

                                                                 gCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACAcc                                                                     >  2:483123/1‑140 (MQ=255)

                                                                  cACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCa                                                                    <  1:644473/140‑1 (MQ=255)

                                                                       ccAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTCTCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGaa                                                               >  1:1893870/1‑140 (MQ=255)

                                                                       ccAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGaa                                                               >  1:1256741/1‑140 (MQ=255)

                                                                         aaCGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGaata                                                             <  2:1079889/140‑1 (MQ=255)

                                                                                aaTAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTa                                                      <  1:2653376/140‑1 (MQ=255)

                                                                                aaTAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTa                                                      <  1:1595426/140‑1 (MQ=255)

                                                                                aaTAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTa                                                      <  1:681164/140‑1 (MQ=255)

                                                                                 aTAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTag                                                     <  1:916591/140‑1 (MQ=255)

                                                                                      ttAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGtg                                                <  2:2622220/140‑1 (MQ=255)

                                                                                        aGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGtgat                                              <  2:1352603/140‑1 (MQ=255)

                                                                                          aaaCATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGtgatga                                            >  1:1593716/1‑140 (MQ=255)

                                                                                           aaCATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAg                                           <  2:2324620/140‑1 (MQ=255)

                                                                                            aCATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGt                                          <  1:2241052/140‑1 (MQ=255)

                                                                                              atatCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTg                                        <  2:62863/140‑1 (MQ=255)

                                                                                              atatCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTg                                        <  1:483123/140‑1 (MQ=255)

                                                                                              atatCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTg                                        <  1:2425474/140‑1 (MQ=255)

                                                                                              atatCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTg                                        <  2:955524/140‑1 (MQ=255)

                                                                                               tatCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGc                                       >  2:1165794/1‑140 (MQ=255)

                                                                                                atCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCt                                      <  1:1532458/140‑1 (MQ=255)

                                                                                                atCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCt                                      <  1:645694/140‑1 (MQ=255)

                                                                                                atCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCt                                      <  2:785903/140‑1 (MQ=255)

                                                                                                  cTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTa                                    >  1:1147651/1‑140 (MQ=255)

                                                                                                  cTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTa                                    <  2:2440170/140‑1 (MQ=255)

                                                                                                  cTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTa                                    <  1:2168670/140‑1 (MQ=255)

                                                                                                    gCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGt                                  <  2:2650385/140‑1 (MQ=255)

                                                                                                        cGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACa                              >  1:2470966/1‑140 (MQ=255)

                                                                                                        cGGATTCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACa                              >  2:256304/1‑140 (MQ=255)

                                                                                                             tCACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACg                         <  2:1258691/140‑1 (MQ=255)

                                                                                                              cACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGa                        <  2:1670760/140‑1 (MQ=255)

                                                                                                              cACGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGa                        <  2:588258/140‑1 (MQ=255)

                                                                                                               aCGTTTATCAGTTTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGAt                       >  1:2630210/1‑140 (MQ=255)

                                                                                                               aCGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGAt                       >  2:862974/1‑140 (MQ=255)

                                                                                                               aCGTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGAt                       >  2:1295846/1‑140 (MQ=255)

                                                                                                                 gTTTATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTg                     <  1:836339/140‑1 (MQ=255)

                                                                                                                  tttATCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGt                    <  2:1790796/140‑1 (MQ=255)

                                                                                                                     aTCAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATg                 >  2:2209128/1‑140 (MQ=255)

                                                                                                                       cAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGAc               <  1:1033665/140‑1 (MQ=255)

                                                                                                                       cAGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGAc               <  1:754915/140‑1 (MQ=255)

                                                                                                                        aGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACg              <  2:967052/140‑1 (MQ=255)

                                                                                                                        aGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACg              <  2:452638/140‑1 (MQ=255)

                                                                                                                        aGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACg              <  1:2087001/140‑1 (MQ=255)

                                                                                                                        aGTGTTGTTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACg              <  1:1054140/140‑1 (MQ=255)

                                                                                                                          tgttgtTTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGaa            <  1:1037435/140‑1 (MQ=255)

                                                                                                                             tgttTTTGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGc         >  1:417922/1‑140 (MQ=255)

                                                                                                                              gttTTTGGGATGTCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCa        <  2:767814/140‑1 (MQ=255)

                                                                                                                              gttTTTGGGATGGCAGCCAGAAGGGAGTCAGGGTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCa        <  1:1692722/140‑1 (MQ=255)

                                                                                                                                  ttGGGATGGCGGCCAGAAGGAAGTCAAGCTAATATTTTGACAATAAACCGGTTGCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAAc    <  2:35067/140‑1 (MQ=255)

                                                                                                                                  ttGGGATGGCAGCCAGAAGGGAGTCAGGTTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAAc    <  2:371979/140‑1 (MQ=255)

                                                                                                                                  ttGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAAc    <  1:2362014/140‑1 (MQ=255)

                                                                                                                                  ttGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAAc    <  1:176567/140‑1 (MQ=255)

                                                                                                                                  ttGGGATGGCAGCCAGAAGGGAGTCAGCCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGTCGAAGGCTTaat    <  2:1893870/140‑2 (MQ=255)

                                                                                                                                   tGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACa   <  2:497517/140‑1 (MQ=255)

                                                                                                                                   tGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACa   <  1:1985767/140‑1 (MQ=255)

                                                                                                                                   tGGGATGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACa   <  1:1495394/140‑1 (MQ=255)

AATCAGATAAGGGATGTCATGTATCGCGATGATTCCATAAAAAATCACCAGAAAGACGAAGATCAGCACTCCCAACGCGAAATAGTTTAGAAACATATCTGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACAT  >  NC_000913/3771649‑3771920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A