Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F73 I1 R1	27	59.0	2035004	99.3%	2020758	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,458,689	C→A	A244E (GCG→GAG)	pmbA →	metalloprotease PmbA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,458,689	0	C	A	100.0%	154.4 / NA	45	A244E (GCG→GAG)	pmbA	metalloprotease PmbA
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (23/22); total (23/22)

CGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCTTCCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGCT  >  NC_000913/4458565‑4458824

cGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTAT‑‑CGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCa                                                                                                                         >  2:182829/1‑140 (MQ=255)

              cacCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCaa                                                                                                             >  2:160711/1‑140 (MQ=255)

                 cATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCg                                                                                                          <  1:200686/140‑1 (MQ=255)

                   ttGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCggg                                                                                                        >  1:185013/1‑140 (MQ=255)

                   ttGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCggg                                                                                                        >  1:265134/1‑140 (MQ=255)

                   ttGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCggg                                                                                                        >  1:686719/1‑140 (MQ=255)

                     ggTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑t                                                                                                     >  2:588280/1‑140 (MQ=255)

                     ggTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑t                                                                                                     >  2:338851/1‑140 (MQ=255)

                        cGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGACAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑tttt                                                                                                  >  2:145488/1‑140 (MQ=255)

                        cGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑tttt                                                                                                  >  2:241899/1‑140 (MQ=255)

                           gCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTgg                                                                                               >  1:759201/1‑140 (MQ=255)

                             gATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGc                                                                                             <  1:855722/140‑1 (MQ=255)

                              aTGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCa                                                                                            <  1:103108/140‑1 (MQ=255)

                                  gCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCtg                                                                                        <  2:447272/140‑1 (MQ=255)

                                             aCGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTTGGGCATCTGGTGGGGGCGa                                                                              >  2:855155/1‑140 (MQ=255)

                                                   gagTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCggg                                                                       <  1:671207/140‑1 (MQ=255)

                                                    agTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTTGGGCATCTGGTGGGGGCGATAGCggg                                                                       <  1:855155/140‑1 (MQ=255)

                                                         gTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATc                                                                 >  2:635352/1‑140 (MQ=255)

                                                             gggCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGtt                                                             <  1:92976/140‑1 (MQ=255)

                                                                  gACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGtt                                                             >  1:84445/1‑135 (MQ=255)

                                                                  gACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGtt                                                             <  2:84445/135‑1 (MQ=255)

                                                                   aCTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGt                                                       <  2:572032/140‑1 (MQ=255)

                                                                    cTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTa                                                      <  2:572150/140‑1 (MQ=255)

                                                                       tgCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAAt                                                   >  1:79479/1‑140 (MQ=255)

                                                                         cTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCt                                                 >  2:155334/1‑140 (MQ=255)

                                                                         cTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCt                                                 >  1:744387/1‑140 (MQ=255)

                                                                            gCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTAcc                                              <  2:265134/140‑1 (MQ=255)

                                                                             ccGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCt                                             <  2:759201/140‑1 (MQ=255)

                                                                              cGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCtt                                            >  2:382450/1‑140 (MQ=255)

                                                                                  cTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGAATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCTTCctg                                        <  1:588280/140‑1 (MQ=255)

                                                                                      aTCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCTTCCTGCTgg                                    <  2:254146/140‑1 (MQ=255)

                                                                                      aTCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCTTCCTGCTgg                                    <  1:338851/140‑1 (MQ=255)

                                                                                      aTCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCTTCCTGCTgg                                    >  2:1012883/1‑140 (MQ=255)

                                                                                          cgTCTGTCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCTTCCTGCTGGATTc                                >  1:735320/1‑140 (MQ=255)

                                                                                                tCACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCTTCCTGCTGGATTCGCTggg                          <  1:891238/140‑1 (MQ=255)

                                                                                                 cACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCTTCCTGCTGGATTCGCTGGGt                         >  1:910761/1‑140 (MQ=255)

                                                                                                 cACCGCGTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCTTCCTGCTGGATTCGCTGGGt                         <  1:1028/140‑1 (MQ=255)

                                                                                                      cgTAAACTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCTTCCTGCTGGATTCGCTGGGTAAACa                    >  1:638941/1‑140 (MQ=255)

                                                                                                          aaCTCTCCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCTTCCTGCTGGATTCGCTGGGTAAACAAAtt                >  2:359880/1‑140 (MQ=255)

                                                                                                             tctcCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCTTCCTGCTGGATTCGCTGGGTAAACAAATTCTg             <  2:686719/140‑1 (MQ=255)

                                                                                                              ctcCACCATGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCTTCCTGCTGGATTCGCTGGGTAAACAAATTCTGc            <  1:359880/140‑1 (MQ=255)

                                                                                                                ccaccaTGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCTTCCTGCTGGATTCGCTGGGTAAACAAATTCTGCCg          >  2:185584/1‑140 (MQ=255)

                                                                                                                      tGAAAGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCTTCCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTgg    <  2:79479/140‑1 (MQ=255)

                                                                                                                        aaaGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCTTCCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGCt  <  1:182829/140‑1 (MQ=255)

                                                                                                                        aaaGAGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCTTCCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGCt  <  1:160711/140‑1 (MQ=255)

CGTGATTACGCCTACACCATTGGTCGTGCGATGAGCGATCTGCAAACGCCAGAGTGGGTTGGGGCCGACTGTGCTCGCCGTACTTTATCGCGTCTGTCACCGCGTAAACTCTCCACCATGAAAGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGC‑TTTTTGGGCATCTGGTGGGGGCGATAGCGGGTGGATCGGTTTATCGTAAATCTACCTTCCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGCT  >  NC_000913/4458565‑4458824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A