Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A9 F73 I1 R1	30	54.0	1878228	99.2%	1863202	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,546,319	G→T	intergenic (+120/‑240)	nfuA → / → gntT	iron‑sulfur cluster carrier protein NfuA/high‑affinity gluconate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,546,319	0	G	T	97.4%	266.8 / 0.4	76	intergenic (+120/‑240)	nfuA/gntT	iron‑sulfur cluster carrier protein NfuA/high‑affinity gluconate transporter
Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (36/38); total (37/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.31e-01

AACACTCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAG  >  NC_000913/3546183‑3546451

aaCACTCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCCTTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGa                                                                                                                                   <  2:679153/140‑1 (MQ=255)

       ctactactaAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATg                                                                                                                            >  2:206099/1‑140 (MQ=255)

               aaGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACct                                                                                                                    >  1:371105/1‑140 (MQ=255)

               aaGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACct                                                                                                                    >  1:316390/1‑140 (MQ=255)

               aaGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACct                                                                                                                    >  1:528605/1‑140 (MQ=255)

               aaGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACct                                                                                                                    >  2:100634/1‑140 (MQ=255)

                 gTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACctct                                                                                                                  <  2:248746/140‑1 (MQ=255)

                 gTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACctct                                                                                                                  <  1:152086/140‑1 (MQ=255)

                 gTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACctct                                                                                                                  >  2:481427/1‑140 (MQ=255)

                     tCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCa                                                                                                                <  2:877496/138‑1 (MQ=255)

                     tCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCa                                                                                                                >  1:877496/1‑138 (MQ=255)

                     tCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATa                                                                                                              >  1:194990/1‑140 (MQ=255)

                     tCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATa                                                                                                              >  1:797711/1‑140 (MQ=255)

                     tCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATa                                                                                                              >  1:285210/1‑140 (MQ=255)

                        ccTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATaatt                                                                                                           <  1:568625/140‑1 (MQ=255)

                          tctTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATaattta                                                                                                         >  1:368725/1‑140 (MQ=255)

                           ctTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATaatttaa                                                                                                        >  1:152105/1‑140 (MQ=255)

                             tttGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATaatttaaat                                                                                                      <  2:368725/140‑1 (MQ=255)

                                  aGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTAcc                                                                                                 <  1:67482/140‑1 (MQ=255)

                                  aGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTAcc                                                                                                 <  1:414505/140‑1 (MQ=255)

                                   gATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATaa                                                                                                             >  1:312705/1‑127 (MQ=255)

                                   gATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATaa                                                                                                             <  2:312705/127‑1 (MQ=255)

                                    aTTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTAcccc                                                                                               <  2:194990/140‑1 (MQ=255)

                                      tGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGc                                                                                             >  1:208012/1‑140 (MQ=255)

                                      tGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTCACCCCGc                                                                                             >  2:785313/1‑140 (MQ=255)

                                       gCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGct                                                                                            <  1:740463/140‑1 (MQ=255)

                                        cccGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGctc                                                                                           >  2:910476/1‑140 (MQ=255)

                                        cccGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGctc                                                                                           >  2:364892/1‑140 (MQ=255)

                                        cccGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGctc                                                                                           >  2:51327/1‑140 (MQ=255)

                                        cccGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGctc                                                                                           >  2:640529/1‑140 (MQ=255)

                                        cccGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGctc                                                                                           >  1:117439/1‑140 (MQ=255)

                                                    cTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCa                                                                               <  2:371105/140‑1 (MQ=255)

                                                         gCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGc                                                                          <  1:656257/140‑1 (MQ=255)

                                                           gTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCa                                                                        <  1:206099/140‑1 (MQ=255)

                                                              aTTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGAt                                                                     >  2:366606/1‑140 (MQ=255)

                                                               tttCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATg                                                                    <  1:7676/140‑1 (MQ=255)

                                                                ttCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTCACCCCGCTCTGGTGATTCTCAAACGCCAGATGt                                                                   <  1:785313/140‑1 (MQ=255)

                                                                      ccTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCg                                                             <  2:592282/140‑1 (MQ=255)

                                                                        tACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTa                                                           >  2:889135/1‑140 (MQ=255)

                                                                         aCGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTAt                                                          >  1:308133/1‑140 (MQ=255)

                                                                          cGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATc                                                         <  2:308133/140‑1 (MQ=255)

                                                                          cGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATc                                                         >  2:296403/1‑140 (MQ=255)

                                                                                  aTGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACAt                                                 <  1:713144/140‑1 (MQ=255)

                                                                                  aTGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACAt                                                 <  2:285210/140‑1 (MQ=255)

                                                                                    gtgCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTa                                                           <  1:168419/128‑1 (MQ=255)

                                                                                    gtgCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTa                                                           >  2:168419/1‑128 (MQ=255)

                                                                                    gtgCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATgg                                               >  1:297743/1‑140 (MQ=255)

                                                                                    gtgCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATgg                                               >  1:609627/1‑140 (MQ=255)

                                                                                    gtgCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATgg                                               >  2:805862/1‑140 (MQ=255)

                                                                                      gCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGt                                             <  1:100634/140‑1 (MQ=255)

                                                                                      gCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGt                                             <  1:889135/140‑1 (MQ=255)

                                                                                           aCGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCaa                                        >  1:252110/1‑140 (MQ=255)

                                                                                           aCGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCaa                                        >  2:746608/1‑140 (MQ=255)

                                                                                            cGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCaaa                                       >  2:723889/1‑140 (MQ=255)

                                                                                            cGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCaaa                                       >  2:525796/1‑140 (MQ=255)

                                                                                             gTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAAc                                      >  1:905395/1‑140 (MQ=255)

                                                                                              ttGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACa                                     <  2:639601/140‑1 (MQ=255)

                                                                                              ttGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACa                                     <  1:723889/140‑1 (MQ=255)

                                                                                              ttGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACa                                     <  2:797711/140‑1 (MQ=255)

                                                                                              ttGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACa                                     <  2:297743/140‑1 (MQ=255)

                                                                                              ttGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACa                                     <  1:525796/140‑1 (MQ=255)

                                                                                                ggCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATa                                   >  1:196210/1‑140 (MQ=255)

                                                                                                ggCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATa                                   <  2:208012/140‑1 (MQ=255)

                                                                                                          tGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACAt                         <  2:511488/140‑1 (MQ=255)

                                                                                                          tGCTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACAt                         <  1:102692/140‑1 (MQ=255)

                                                                                                            cTTTGTCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCt                       <  2:55022/140‑1 (MQ=255)

                                                                                                                 tCAAATCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTa                  >  2:490125/1‑140 (MQ=255)

                                                                                                                   aaaTCCTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACa                <  1:805862/140‑1 (MQ=255)

                                                                                                                       ccTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACaataa            <  2:609627/140‑1 (MQ=255)

                                                                                                                        cTCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACaataat           <  2:522905/140‑1 (MQ=255)

                                                                                                                         tCGGTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAAtt          <  2:905395/140‑1 (MQ=255)

                                                                                                                           ggTTCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAg        >  2:686954/1‑140 (MQ=255)

                                                                                                                              tCGTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGttt     <  1:296403/140‑1 (MQ=255)

                                                                                                                                gTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTa   <  1:640529/140‑1 (MQ=255)

                                                                                                                                gTTTCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTa   <  1:364892/140‑1 (MQ=255)

                                                                                                                                 tttCCCCTCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAg  >  2:564950/1‑140 (MQ=255)

AACACTCCTACTACTAAGTTATCCCCTCTTTTGTAGATTGCCCGATGCGACGCTAAAGCGTCATTTCGGGCCTACGTTGAACATGTGCCAGACGTTGGCGCAGCATTGCTTTGTCAAATCCTCGGTTCGTTTCCCCGCGATAATATGACCAACCTCTCATAATTTAAATTTACCCCGCTCTGGTGATTCTCAAACGCCAGATGTTACCCGTATCATTCACATGGGTACCAAACATACTCCTGACATCTGACTACAATAATTAGTTTTAG  >  NC_000913/3546183‑3546451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A