Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F73 I1 R1	23	178.8	6243766	99.3%	6200059	139.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,984,980	G→T	Q205K (CAA→AAA)	yqeF ←	putative acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,984,980	0	G	T	98.3%	619.5 / 2.8	174	Q205K (CAA→AAA)	yqeF	putative acyltransferase
Reads supporting (aligned to +/- strand): ref base G (3/0); new base T (99/72); total (102/72)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 176 total aligned reads displayed.

TTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTGATGCCATATTCGCGAGCCAGATTTTCGGCGGTG  >  NC_000913/2984844‑2985116

 ttATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTc                                                                                                                                      <  2:1853058/140‑1 (MQ=255)

  tATGGATGATGCATTACCCGCTGTCATAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGTGTCAGTGCGTGGCTGTTCACCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCa                                                                                                                                     <  2:1463238/140‑1 (MQ=255)

  tATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCa                                                                                                                                     <  1:2210900/140‑1 (MQ=255)

       atgatgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTAcc                                                                                                                                <  2:216555/140‑1 (MQ=255)

         gatgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCgg                                                                                                                              >  1:556836/1‑140 (MQ=255)

         gatgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCgg                                                                                                                              >  2:2593286/1‑140 (MQ=255)

         gatgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCgg                                                                                                                              >  1:1380238/1‑140 (MQ=255)

          atgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCggg                                                                                                                             <  1:2593286/140‑1 (MQ=255)

          atgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCggg                                                                                                                             >  2:1133455/1‑140 (MQ=255)

          atgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCggg                                                                                                                             >  1:2205635/1‑140 (MQ=255)

           tgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTAc                                                                                                                                 <  1:952907/135‑1 (MQ=255)

           tgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGa                                                                                                                            >  1:2055810/1‑140 (MQ=255)

           tgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGa                                                                                                                            >  1:2682947/1‑140 (MQ=255)

           tgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGa                                                                                                                            >  1:2799052/1‑140 (MQ=255)

           tgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGa                                                                                                                            <  2:473011/140‑1 (MQ=255)

           tgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGa                                                                                                                            >  2:74490/1‑140 (MQ=255)

           tgCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGa                                                                                                                            >  2:2049270/1‑140 (MQ=255)

              aTTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTGCCGGGACGa                                                                                                                         >  1:533200/1‑140 (MQ=255)

                 aCCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGAtct                                                                                                                      <  1:1006536/140‑1 (MQ=255)

                 aCCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGAtct                                                                                                                      <  1:1619388/140‑1 (MQ=255)

                 aCCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGAtct                                                                                                                      <  2:2277037/140‑1 (MQ=255)

                 aCCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGCCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGAtct                                                                                                                      <  2:1952201/140‑1 (MQ=255)

                  cccGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGt                                                                                                                                       <  1:2184667/122‑1 (MQ=255)

                  cccGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGt                                                                                                                                       >  2:2184667/1‑122 (MQ=255)

                  cccGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGAtctc                                                                                                                     >  2:1382367/1‑140 (MQ=255)

                     gCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCAGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATc                                                                                                                  >  2:667009/1‑140 (MQ=255)

                      cTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCt                                                                                                                 >  1:1226906/1‑140 (MQ=255)

                       tGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCtt                                                                                                                >  1:861273/1‑140 (MQ=255)

                         tCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTa                                                                                                              >  2:907988/1‑140 (MQ=255)

                                 ccGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTcc                                                                                                      >  2:1606919/1‑140 (MQ=255)

                                 ccGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTcc                                                                                                      >  2:1731539/1‑140 (MQ=255)

                                         aTCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCaa                                                                                              >  1:2274919/1‑140 (MQ=255)

                                         aTCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCaa                                                                                              >  2:1074593/1‑140 (MQ=255)

                                         aTCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCaa                                                                                              >  1:419977/1‑140 (MQ=255)

                                           cAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGTGACGATCTCATCTTTAAATCGTCCTGCGTCAATc                                                                                            >  2:3061649/1‑140 (MQ=255)

                                              aTGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATcgcc                                                                                         >  1:1091194/1‑140 (MQ=255)

                                               tGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATcgccg                                                                                        >  2:2406556/1‑140 (MQ=255)

                                               tGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATcgccg                                                                                        >  2:781203/1‑140 (MQ=255)

                                                gAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTGCCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATcgccgc                                                                                       <  2:533200/140‑1 (MQ=255)

                                                gAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATcgccgc                                                                                       >  1:2652613/1‑140 (MQ=255)

                                                 aaGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCt                                                                                      <  1:2406556/140‑1 (MQ=255)

                                                 aaGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCt                                                                                      <  1:3082393/140‑1 (MQ=255)

                                                  aGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTc                                                                                     >  1:3026518/1‑140 (MQ=255)

                                                  aGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTc                                                                                     <  1:1260920/140‑1 (MQ=255)

                                                   ggATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTcg                                                                                    <  2:1367713/140‑1 (MQ=255)

                                                    gATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTcgc                                                                                   >  2:288537/1‑140 (MQ=255)

                                                    gATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTcgc                                                                                   >  2:535810/1‑140 (MQ=255)

                                                    gATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTcgc                                                                                   >  1:2311017/1‑140 (MQ=255)

                                                      tttAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCgcgc                                                                                 >  2:2755371/1‑140 (MQ=255)

                                                        tAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCtt                                                                               >  1:2053756/1‑140 (MQ=255)

                                                               gCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTttgttg                                                                        <  2:2799052/140‑1 (MQ=255)

                                                               gCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTttgttg                                                                        >  2:2392171/1‑140 (MQ=255)

                                                                   aGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAg                                                                    <  2:861273/140‑1 (MQ=255)

                                                                   aGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAg                                                                    <  2:7267/140‑1 (MQ=255)

                                                                   aGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAg                                                                    >  1:2548071/1‑140 (MQ=255)

                                                                   aGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAg                                                                    <  1:2482376/140‑1 (MQ=255)

                                                                    gCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCtt                                                                               <  2:288104/128‑1 (MQ=255)

                                                                    gCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCtt                                                                               >  1:288104/1‑128 (MQ=255)

                                                                     ccTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCt                                                                  <  2:2311017/140‑1 (MQ=255)

                                                                     ccTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCt                                                                  <  1:1011799/140‑1 (MQ=255)

                                                                      cTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTa                                                                 <  2:2517723/140‑1 (MQ=255)

                                                                      cTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTa                                                                 <  2:755754/140‑1 (MQ=255)

                                                                      cTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTa                                                                 <  1:63851/140‑1 (MQ=255)

                                                                         cTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGt                                                              >  2:401977/1‑140 (MQ=255)

                                                                         cTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGt                                                              >  2:2327157/1‑140 (MQ=255)

                                                                         cTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGt                                                              >  2:1817075/1‑140 (MQ=255)

                                                                         cTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGt                                                              >  2:1902995/1‑140 (MQ=255)

                                                                         cTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGt                                                              >  1:159438/1‑140 (MQ=255)

                                                                         cTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGt                                                              >  2:2776132/1‑140 (MQ=255)

                                                                               tGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCt                                                                  >  1:2827054/1‑130 (MQ=255)

                                                                                 gCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGc                                                      >  2:127330/1‑140 (MQ=255)

                                                                                 gCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGc                                                      >  1:1615424/1‑140 (MQ=255)

                                                                                       gTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTg                                                <  2:1013023/140‑1 (MQ=255)

                                                                                         gCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCa                                              <  1:1382367/140‑1 (MQ=255)

                                                                                           gTGGCTGTTCAACGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGCCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAAc                                            >  1:1545903/1‑140 (MQ=255)

                                                                                              gCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGa                                         <  1:1189039/140‑1 (MQ=255)

                                                                                              gCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGa                                         >  1:2219086/1‑140 (MQ=255)

                                                                                              gCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGa                                         <  1:299266/140‑1 (MQ=255)

                                                                                                  ttCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGc                                     >  2:2137990/1‑140 (MQ=255)

                                                                                                  ttCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGc                                     >  2:1103767/1‑140 (MQ=255)

                                                                                                  ttCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGc                                     >  2:2839679/1‑140 (MQ=255)

                                                                                                  ttCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTATAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAg                                                       <  2:647702/122‑1 (MQ=255)

                                                                                                   tCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCt                                    <  2:491449/140‑1 (MQ=255)

                                                                                                    cATCGGTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTg                                   >  1:2939310/1‑140 (MQ=255)

                                                                                                         gTATCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTGATGcc                              >  2:94876/1‑140 (MQ=255)

                                                                                                            tCAACAACCAACGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTGATGCCata                           >  2:1130536/1‑140 (MQ=255)

                                                                                                                  accaacGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTGATGCCATATTcgcg                     >  1:508188/1‑140 (MQ=255)

                                                                                                                  accaacGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTGATGCCATATTcgcg                     >  1:744805/1‑140 (MQ=255)

                                                                                                                  accaacGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTGATGCCATATTcgcg                     <  1:1133455/140‑1 (MQ=255)

                                                                                                                    caacGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTGATGCCATATTCGCGAg                   >  1:483492/1‑140 (MQ=255)

                                                                                                                     aacGTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTGATGCCATATTCGCGAGc                  <  1:1884374/140‑1 (MQ=255)

                                                                                                                      acGTCTGCCCGTTACTTTTGGTCA‑TACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTGATGCCATATTCGCGAGCCa                >  1:90275/1‑140 (MQ=255)

                                                                                                                      acGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTGATGCCATATTCGCGAGcc                 >  1:826689/1‑140 (MQ=255)

                                                                                                                        gTCTGCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTGATGCCATATTCGCGAGCCAg               >  2:1388988/1‑140 (MQ=255)

                                                                                                                            gCCCGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTGATGCCATATTCGCGAGCCAGAttt           >  1:1766271/1‑140 (MQ=255)

                                                                                                                             cccGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTGATGCCATATTCGCGAGCCAGAtttt          >  2:1020548/1‑140 (MQ=255)

                                                                                                                               cGTTACTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTGATGCCATATTCGCGAGCCAGATTTTcg        <  1:1071304/140‑1 (MQ=255)

                                                                                                                                    cTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTGATGCCATATTCGCGAGCCAGATTTTCGGCGGt   <  1:667009/140‑1 (MQ=255)

                                                                                                                                    cTTTTGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTGATGCCATATTCGCGAGCCAGATTTTCGGCGGt   <  1:2137990/140‑1 (MQ=255)

                                                                                                                                     ttttGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTGATGCCATATTCGCGAGCCAGATTTTCGGCGGTg  <  2:1754710/140‑1 (MQ=255)

TTTATGGATGATGCATTACCCGCTGTCACAGAACCGAGACTATCAAATGAAGGATTTAAACGGGCTAAGCCTTCTGCGCTGGCGTCAGTGCGTGGCTGTTCATCGGTATCAACAACCAACGTCTGCCCGTTACTTTGGGTCATTACCGGGACGATCTCATCTTTAAATCGTCCGGCGTCAATCGCCGCTCGCGCTTTTTGTTGCGAGCTAAGTGCGTAAGCATCCTGCAACTGACGGCTGATGCCATATTCGCGAGCCAGATTTTCGGCGGTG  >  NC_000913/2984844‑2985116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A