Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A11 F73 I1 R1	26	148.5	5206078	99.3%	5169635	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,276,558	C→A	S363* (TCG→TAG)	pdeC →	c‑di‑GMP phosphodiesterase PdeC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,276,558	0	C	A	99.4%	603.2 / ‑4.2	169	S363* (TCG→TAG)	pdeC	c‑di‑GMP phosphodiesterase PdeC
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (99/69); minor base T (1/0); total (100/69)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

Only 100 of 170 total aligned reads displayed.

TCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTGATGTTGACAAAATGACGCCGAT  >  NC_000913/4276421‑4276693

tCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAgg                                                                                                                                       <  2:1159810/140‑1 (MQ=255)

   ggCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCt                                                                                                                                    <  2:2393686/140‑1 (MQ=255)

    gCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTc                                                                                                                                   >  2:2322703/1‑140 (MQ=255)

    gCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTc                                                                                                                                   >  1:1360662/1‑140 (MQ=255)

    gCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTc                                                                                                                                   >  1:1840634/1‑140 (MQ=255)

       gagTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGa                                                                                                                                >  1:1612069/1‑140 (MQ=255)

        agTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGAt                                                                                                                               >  2:543153/1‑140 (MQ=255)

         gTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGAtt                                                                                                                              <  2:1259485/140‑1 (MQ=255)

             aTTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCa                                                                                                                          <  1:1919180/140‑1 (MQ=255)

               tCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATCAACCTGTAGGCCTCCGATTTTCATa                                                                                                                        >  2:2529839/1‑140 (MQ=255)

                   cTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTc                                                                                                                    <  2:719129/140‑1 (MQ=255)

                   cTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTc                                                                                                                    >  1:2215635/1‑140 (MQ=255)

                    tGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTc                                                                                                                    >  1:823407/1‑139 (MQ=255)

                    tGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTc                                                                                                                    <  2:823407/139‑1 (MQ=255)

                    tGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCa                                                                                                                   >  2:2166464/1‑140 (MQ=255)

                           aaaGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGa                                                                                                            <  2:1360662/140‑1 (MQ=255)

                                     atgatAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTc                                                                                                                    >  1:2392847/1‑122 (MQ=255)

                                      tgatAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGaatc                                                                                                 <  1:1472088/140‑1 (MQ=255)

                                        atAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGaatcaa                                                                                               >  1:1003921/1‑140 (MQ=255)

                                        atAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGaatcaa                                                                                               >  1:1026468/1‑140 (MQ=255)

                                             aCAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGaatc                                                                                                 >  1:649005/1‑133 (MQ=255)

                                             aCAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGaatc                                                                                                 <  2:649005/133‑1 (MQ=255)

                                                gATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGaaaa                                                                                       <  1:2249518/140‑1 (MQ=255)

                                                gATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGaaaa                                                                                       <  2:134021/140‑1 (MQ=255)

                                                 aTAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGGTCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAAc                                                                                      >  2:1747393/1‑140 (MQ=255)

                                                 aTAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGaatc                                                                                                 >  1:2062090/1‑129 (MQ=255)

                                                 aTAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAAc                                                                                      >  2:2060043/1‑140 (MQ=255)

                                                 aTAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAAc                                                                                      >  2:1795300/1‑140 (MQ=255)

                                                 aTAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAAc                                                                                      >  1:1399235/1‑140 (MQ=255)

                                                  tAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACa                                                                                     <  1:1866719/140‑1 (MQ=255)

                                                     cTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACagag                                                                                  <  1:2529839/140‑1 (MQ=255)

                                                        aTTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCaa                                                                               >  2:1772388/1‑140 (MQ=255)

                                                        aTTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCaa                                                                               >  1:1944200/1‑140 (MQ=255)

                                                                aTTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGt                                                                       >  2:2037148/1‑140 (MQ=255)

                                                                aTTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGt                                                                       >  2:45092/1‑140 (MQ=255)

                                                                   gATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCg                                                                    >  2:2386561/1‑140 (MQ=255)

                                                                    aTAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGt                                                                   >  1:672554/1‑140 (MQ=255)

                                                                      aaTGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATa                                                                             <  2:223645/128‑1 (MQ=255)

                                                                      aaTGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATa                                                                             >  1:223645/1‑128 (MQ=255)

                                                                      aaTGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTcc                                                                 >  1:284233/1‑140 (MQ=255)

                                                                        tGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCgc                                                               >  1:290600/1‑140 (MQ=255)

                                                                        tGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCgc                                                               >  1:1393399/1‑140 (MQ=255)

                                                                        tGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCgc                                                               >  1:1261437/1‑140 (MQ=255)

                                                                         gTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCgca                                                              >  2:895788/1‑140 (MQ=255)

                                                                           cTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCgcagc                                                            >  1:2427158/1‑140 (MQ=255)

                                                                            ttCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCgcagca                                                           >  2:686834/1‑140 (MQ=255)

                                                                            ttCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCgcagca                                                           >  1:1766305/1‑140 (MQ=255)

                                                                             tCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCaa                                                          >  1:1605481/1‑140 (MQ=255)

                                                                             tCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCaa                                                          >  1:2334375/1‑140 (MQ=255)

                                                                              ccGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCaaa                                                         >  1:2422679/1‑140 (MQ=255)

                                                                                  gATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCaaattaa                                                     >  2:1639125/1‑140 (MQ=255)

                                                                                   aTCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCaaattaaa                                                    <  2:1840634/140‑1 (MQ=255)

                                                                                    tCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCaaattaaat                                                   >  1:753457/1‑140 (MQ=255)

                                                                                      tGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAAttt                                                 >  2:337934/1‑140 (MQ=255)

                                                                                       gggCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTg                                                <  2:1766305/140‑1 (MQ=255)

                                                                                       gggCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTg                                                <  2:1612069/140‑1 (MQ=255)

                                                                                       gggCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTg                                                <  2:753457/140‑1 (MQ=255)

                                                                                       gggCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTg                                                <  1:2037148/140‑1 (MQ=255)

                                                                                         gCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGaa                                              <  1:337934/140‑1 (MQ=255)

                                                                                         gCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGaa                                              <  2:1393399/140‑1 (MQ=255)

                                                                                         gCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGaa                                              <  2:1575194/140‑1 (MQ=255)

                                                                                           gATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTg                                                >  2:2132661/1‑136 (MQ=255)

                                                                                             ttACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGa                                          <  1:606365/140‑1 (MQ=255)

                                                                                             ttACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGa                                          <  1:2060043/140‑1 (MQ=255)

                                                                                             ttACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGa                                          <  1:86353/140‑1 (MQ=255)

                                                                                              tACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGAc                                         <  1:2153928/140‑1 (MQ=255)

                                                                                              tACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAACAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGAc                                         <  1:1271787/140‑1 (MQ=255)

                                                                                                 cTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGa                                      <  1:1772388/140‑1 (MQ=255)

                                                                                                  tGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAg                                     >  1:1409114/1‑140 (MQ=255)

                                                                                                   ggCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAgc                                    >  2:579444/1‑140 (MQ=255)

                                                                                                   ggCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAgc                                    >  2:1671614/1‑140 (MQ=255)

                                                                                                    gCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAgca                                   <  2:1026468/140‑1 (MQ=255)

                                                                                                    gCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAgca                                   <  1:1639125/140‑1 (MQ=255)

                                                                                                    gCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAgca                                   <  2:1003921/140‑1 (MQ=255)

                                                                                                    gCAACACATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAgca                                   <  1:1795300/140‑1 (MQ=255)

                                                                                                       acacATGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAgcatgc                                >  1:1945496/1‑140 (MQ=255)

                                                                                                          caTGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCAtt                             >  2:2112869/1‑140 (MQ=255)

                                                                                                           aTGCAGATCGCTATGTTTCTATTAACCTGTTGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCAttt                            >  1:47475/1‑140 (MQ=255)

                                                                                                            tGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTc                           >  2:2064141/1‑140 (MQ=255)

                                                                                                            tGCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTc                           <  1:52928/140‑1 (MQ=255)

                                                                                                             gCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGAc                                         >  2:1194635/1‑125 (MQ=255)

                                                                                                             gCAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGAc                                         <  1:1194635/125‑1 (MQ=255)

                                                                                                              cAGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCtt                         >  1:714947/1‑140 (MQ=255)

                                                                                                               aGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTg                        <  1:739245/140‑1 (MQ=255)

                                                                                                               aGATCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTg                        <  2:2215635/140‑1 (MQ=255)

                                                                                                                 aTCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTGAt                      <  2:1937266/140‑1 (MQ=255)

                                                                                                                 aTCGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTGAt                      >  2:230016/1‑140 (MQ=255)

                                                                                                                   cGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTGATGt                    >  2:1969138/1‑140 (MQ=255)

                                                                                                                   cGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTGATGt                    >  2:1492806/1‑140 (MQ=255)

                                                                                                                   cGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTGATGt                    >  1:407978/1‑140 (MQ=255)

                                                                                                                   cGCTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTGATGt                    <  2:820189/140‑1 (MQ=255)

                                                                                                                     cTATGTTTCTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTGATGTTg                  >  2:1406852/1‑140 (MQ=255)

                                                                                                                             cTATTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTGa                       <  2:2246976/127‑1 (MQ=255)

                                                                                                                               aTTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTGATGTTGACAAAATGAc        <  2:2589680/140‑1 (MQ=255)

                                                                                                                               aTTAACCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTGATGTTGACAAAATGAc        >  2:89048/1‑140 (MQ=255)

                                                                                                                                  aaCCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTGATGTTGACAAAATGACGcc     >  2:1070954/1‑140 (MQ=255)

                                                                                                                                   aCCTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTGATGTTGACAAAATGACGCCg    <  2:1852317/140‑1 (MQ=255)

                                                                                                                                    ccTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTGATGTTGACAAAATGAc        >  1:2284204/1‑135 (MQ=255)

                                                                                                                                    ccTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTGATGTTGACAAAATGACGCCGa   >  1:2168132/1‑140 (MQ=255)

                                                                                                                                    ccTGTAGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTGATGTTGACAAAATGACGCCGa   >  1:1808443/1‑140 (MQ=255)

TCCGGCAGAGTTTATTCCGCTGGCAGAAAAGGAGGGGATGATAGAACAGATAACTGATTATGTTATTGATAATGTCTTCCGCGATCTGGGCGATTACCTGGCAACACATGCAGATCGCTATGTTTCTATTAACCTGTCGGCCTCCGATTTTCATACGTCACGGTTGATAGCGCGAATCAATCAGAAAACAGAGCAATACGCGGTGCGTCCGCAGCAAATTAAATTTGAAGTGACTGAGCATGCATTTCTTGATGTTGACAAAATGACGCCGAT  >  NC_000913/4276421‑4276693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A