Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	124	90.1	3003320	99.2%	2979293	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	607,055	A→G	V110A (GTA→GCA)	ybdK ←	carboxylate‑amine ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	607,055	0	A	G	100.0%	164.9 / NA	46	V110A (GTA→GCA)	ybdK	carboxylate‑amine ligase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/15); total (31/15)

GCCGTGCAGCAAATAAATGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCAAA  >  NC_000913/606925‑607190

gCCGTGCAGCAAATAAATGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGAc                                                                                                                                >  2:762149/1‑140 (MQ=255)

     gcagcaAATAAATGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGc                                                                                                                           <  1:727187/140‑1 (MQ=255)

      cagcaAATAAATGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGcc                                                                                                                          >  2:1081010/1‑140 (MQ=255)

       agcaAATAAATGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCACATGCCTCCTGACGCTGCCa                                                                                                                         <  1:478453/140‑1 (MQ=255)

         caAATAAATGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCAtt                                                                                                                       <  1:3528/140‑1 (MQ=255)

           aataaatGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTc                                                                                                                     >  2:458869/1‑140 (MQ=255)

               aatGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGaa                                                                                                                 <  1:1312630/140‑1 (MQ=255)

                atGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGaaa                                                                                                                <  2:153551/140‑1 (MQ=255)

                   gCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACgg                                                                                                             <  2:721817/140‑1 (MQ=255)

                   gCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACgg                                                                                                             >  1:403655/1‑140 (MQ=255)

                   gCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACgg                                                                                                             <  1:559346/140‑1 (MQ=255)

                      tCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGgtg                                                                                                          <  1:1433896/140‑1 (MQ=255)

                        aTCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGgtgcg                                                                                                        >  2:1295827/1‑140 (MQ=255)

                              aCTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCAc                                                                                                  >  2:1302962/1‑140 (MQ=255)

                                   cgcAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCAccgccg                                                                                             >  2:524372/1‑140 (MQ=255)

                                    gcAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCTTTTCTGAAACGGGTGCGTGCCACcgccgc                                                                                            <  2:788564/140‑1 (MQ=255)

                                    gcAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACcgccgc                                                                                            >  2:737055/1‑140 (MQ=255)

                                     cAGCCAACATGGACATGCTGACCAAAACCGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCa                                                                                           <  1:190046/140‑1 (MQ=255)

                                            cATGGTCATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTcc                                                                                    >  2:1089574/1‑140 (MQ=255)

                                                gACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAgat                                                                                >  2:677576/1‑140 (MQ=255)

                                                  cATGCTGCCAAAAACCGGTCGCCTGCTGAAGGACATACCCAAGGTTGTCCGGCGTGCGTTGATAGCGTTCGTTTTCGCATGCCCCCCGGCCCTGCAATTTTTGAAACGGGTGCGTCCCACCGCCGCAAATTTCCAgatga                                                                              <  2:12915/140‑1 (MQ=255)

                                                  cATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAgatga                                                                              >  1:1063013/1‑140 (MQ=255)

                                                  cATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAgatga                                                                              <  1:594630/140‑1 (MQ=255)

                                                  cATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAgatga                                                                              >  2:1359013/1‑140 (MQ=255)

                                                  cATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAgatga                                                                              >  2:833228/1‑140 (MQ=255)

                                                    tGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGatgatg                                                                            >  2:1465736/1‑140 (MQ=255)

                                                     gCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATgg                                                                           >  2:1467353/1‑140 (MQ=255)

                                                      cTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAATTTTCCAGATGATGGt                                                                          >  2:260109/1‑140 (MQ=255)

                                                      cTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGt                                                                          >  1:694174/1‑140 (MQ=255)

                                                        gACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCt                                                                        >  1:1407527/1‑140 (MQ=255)

                                                             aaaaCGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATGGCGTTCGTTACCGCATGCCTCCTGACGCTGCCTTTTCTGAAAGGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGc                                                                   <  2:350002/140‑1 (MQ=255)

                                                             aaaaCGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGc                                                                   >  2:325408/1‑140 (MQ=255)

                                                              aaaCGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGct                                                                  >  2:554440/1‑140 (MQ=255)

                                                                   gTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGctgcctg                                                             >  2:286363/1‑140 (MQ=255)

                                                                   gTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGctgcctg                                                             <  1:1081010/140‑1 (MQ=255)

                                                                         tgctgAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATAc                                                       >  2:1197595/1‑140 (MQ=255)

                                                                          gctgAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACg                                                      <  2:133020/140‑1 (MQ=255)

                                                                                  agaTAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTg                                              <  1:505439/140‑1 (MQ=255)

                                                                                        ccAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGc                                        >  2:95224/1‑140 (MQ=255)

                                                                                        ccAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGc                                        >  2:570420/1‑140 (MQ=255)

                                                                                             gTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGaaa                                   >  2:1177966/1‑140 (MQ=255)

                                                                                                        tgcgtTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCa                        >  1:316854/1‑140 (MQ=255)

                                                                                                        tgcgtTGATAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCa                        >  2:904205/1‑140 (MQ=255)

                                                                                                          cgtTGATAGCGTTCGTTACCGCATGCCCCCTGACGCTGCCATTTCTGAAGCGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGc                      <  2:403655/140‑1 (MQ=255)

                                                                                                               aTAGCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTTCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGt                 <  2:1063013/140‑1 (MQ=255)

                                                                                                                  gCGTTCGTTATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGa              >  2:1489699/1‑140 (MQ=255)

                                                                                                                      tcgttATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATc          >  2:1428326/1‑140 (MQ=255)

                                                                                                                       cgttATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCa         >  2:1471906/1‑140 (MQ=255)

                                                                                                                       cgttATCGCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCa         >  2:1333124/1‑140 (MQ=255)

                                                                                                                       cgttATCGCATGCCTCCTGACGCCGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCa         >  2:863238/1‑140 (MQ=255)

                                                                                                                              gCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCaaa  <  1:833228/140‑1 (MQ=255)

                                                                                                                              gCATGCCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCaaa  <  1:1471906/140‑1 (MQ=255)

GCCGTGCAGCAAATAAATGGCGTCATCGCCACTGGCGCAGCCAACATGGACATGCTGACCAAAAACGGTCGCCTGCTGAATGAGATAACCAAAGTTTTCCAGCGTGCGTTGATAGCGTTCGTTATCGCATACCTCCTGACGCTGCCATTTCTGAAACGGGTGCGTGCCACCGCCGCAAATTTCCAGATGATGGTCTGTGGCTGCCTGCAATACGACTTTCTGCATCGCTGAAAACTGCCCGGCAGCCTGGTTGATATCACGGCAAA  >  NC_000913/606925‑607190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A