Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	124	90.1	3003320	99.2%	2979293	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,815,787	A→G	T641A (ACT→GCT)	katE →	catalase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,815,787	0	A	G	100.0%	306.6 / NA	81	T641A (ACT→GCT)	katE	catalase II
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (45/36); total (45/36)

GGTGATGTGAAAGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGACTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGCGATGCCAACTACTACCTGA  >  NC_000913/1815652‑1815922

ggTGATGTGAAAGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGc                                                                                                                                     >  2:1087762/1‑140 (MQ=255)

  tGATGTGAAAGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCgg                                                                                                                                   >  2:952538/1‑140 (MQ=255)

  tGATGTGAAAGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCgg                                                                                                                                   >  1:1208529/1‑140 (MQ=255)

  tGATGTGAAAGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCgg                                                                                                                                   >  2:1039620/1‑140 (MQ=255)

  tGATGTGAAAGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCgg                                                                                                                                   >  1:376519/1‑140 (MQ=255)

   gATGTGAAAGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGa                                                                                                                                  <  2:931635/140‑1 (MQ=255)

    aTGTGAAAGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGAt                                                                                                                                 >  2:762820/1‑140 (MQ=255)

      gtgAAAGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGGGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGa                                                                                                                               <  1:938923/140‑1 (MQ=255)

      gtgAAAGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCACAGGCGTTCATGCCAAACTGCTCTACTCCCAAATGGGTGAAGTGGCTGCGGATGa                                                                                                                               <  2:1099493/140‑1 (MQ=255)

      gtgAAAGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTGCTCCCGAATGGGTGAAGTGGCTGCGGATGa                                                                                                                               <  2:908151/140‑1 (MQ=255)

       tgAAAGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGac                                                                                                                              >  2:942731/1‑140 (MQ=255)

          aaGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGacggt                                                                                                                           <  1:1058375/140‑1 (MQ=255)

           aGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGacggta                                                                                                                          >  2:46348/1‑140 (MQ=255)

           aGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGacggta                                                                                                                          <  2:968051/140‑1 (MQ=255)

           aGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGacggta                                                                                                                          <  1:1189302/140‑1 (MQ=255)

           aGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGacggta                                                                                                                          <  1:1227395/140‑1 (MQ=255)

           aGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGacggta                                                                                                                          <  1:633201/140‑1 (MQ=255)

             gTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGacggtacg                                                                                                                        <  1:840546/140‑1 (MQ=255)

             gTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGacggtacg                                                                                                                        <  2:860520/140‑1 (MQ=255)

              tCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGacggtacgg                                                                                                                       >  1:659944/1‑140 (MQ=255)

                 cgTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGgtgt                                                                                                                    >  1:1399035/1‑140 (MQ=255)

                 cgTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGgtgt                                                                                                                    >  1:1088686/1‑140 (MQ=255)

                      tAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCt                                                                                                               <  1:697257/140‑1 (MQ=255)

                         cGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCtata                                                                                                            >  2:1248049/1‑140 (MQ=255)

                             tttACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCg                                                                                                        <  2:993076/140‑1 (MQ=255)

                             tttACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCg                                                                                                        <  1:931474/140‑1 (MQ=255)

                             tttACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCg                                                                                                        <  1:350759/140‑1 (MQ=255)

                                           aaGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGt                                                                                          >  1:109514/1‑140 (MQ=255)

                                            aGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTg                                                                                         >  2:1060737/1‑140 (MQ=255)

                                              tGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCa                                                                                       <  1:952538/140‑1 (MQ=255)

                                                 gaTCGGCAGACCTTCTGGCCATTCTCGAGGCGCTGAAGGCCAAAGGGGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCGTTGCCGGTGCACCt                                                                                    >  2:1205279/1‑140 (MQ=255)

                                                  aTCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCtt                                                                                   >  2:1460026/1‑140 (MQ=255)

                                                  aTCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCtt                                                                                   >  2:991216/1‑140 (MQ=255)

                                                  aTCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCtt                                                                                   >  2:536144/1‑140 (MQ=255)

                                                    cGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCg                                                                                 <  1:70659/140‑1 (MQ=255)

                                                      gCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCt                                                                               <  2:590560/140‑1 (MQ=255)

                                                          aCCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACg                                                                           >  2:387946/1‑140 (MQ=255)

                                                            cTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGt                                                                         <  2:30191/140‑1 (MQ=255)

                                                            cTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGt                                                                         >  1:238667/1‑140 (MQ=255)

                                                             ttCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTc                                                                        >  2:428178/1‑140 (MQ=255)

                                                               cTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGa                                                                      >  1:82132/1‑140 (MQ=255)

                                                               cTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGa                                                                      >  1:550262/1‑140 (MQ=255)

                                                               cTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGa                                                                      >  2:485161/1‑140 (MQ=255)

                                                                 ggCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATg                                                                    <  1:762820/140‑1 (MQ=255)

                                                                 ggCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATg                                                                    <  2:376519/140‑1 (MQ=255)

                                                                  gCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGc                                                                   <  2:659944/140‑1 (MQ=255)

                                                                    cATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCgg                                                                 <  1:1039620/140‑1 (MQ=255)

                                                                       tctcAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCa                                                              >  1:1120641/1‑140 (MQ=255)

                                                                       tctcAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCa                                                              >  2:660932/1‑140 (MQ=255)

                                                                         tcAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCAtt                                                            <  2:1393628/140‑1 (MQ=255)

                                                                           aaGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGt                                                          <  2:1088686/140‑1 (MQ=255)

                                                                            aGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTACTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTc                                                         >  1:799985/1‑140 (MQ=255)

                                                                               cgcTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCt                                                      <  1:434637/140‑1 (MQ=255)

                                                                               cgcTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCt                                                      <  1:1087762/140‑1 (MQ=255)

                                                                                gcTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCtt                                                     >  1:55090/1‑140 (MQ=255)

                                                                                 cTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTg                                                    >  2:658442/1‑140 (MQ=255)

                                                                                 cTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTg                                                    >  1:318925/1‑140 (MQ=255)

                                                                                 cTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTg                                                    >  2:906021/1‑140 (MQ=255)

                                                                                      ggCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCa                                               <  2:315088/140‑1 (MQ=255)

                                                                                      ggCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCa                                               <  1:46348/140‑1 (MQ=255)

                                                                                           aaGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATc                                          <  2:231914/140‑1 (MQ=255)

                                                                                                 ttCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGat                                    >  2:280596/1‑140 (MQ=255)

                                                                                                 ttCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGat                                    >  1:1221346/1‑140 (MQ=255)

                                                                                                 ttCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGat                                    >  2:653831/1‑140 (MQ=255)

                                                                                                      gCCAAACTGCTCTACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGCGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGc                               >  2:43706/1‑140 (MQ=255)

                                                                                                               ctctACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACgg                      <  1:717164/140‑1 (MQ=255)

                                                                                                                tctACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGc                     >  2:404694/1‑140 (MQ=255)

                                                                                                                 ctACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGCg                    <  1:1223517/140‑1 (MQ=255)

                                                                                                                 ctACTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGCg                    <  1:9835/140‑1 (MQ=255)

                                                                                                                    cTCCCGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGCGATg                 >  1:17807/1‑140 (MQ=255)

                                                                                                                      cccGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGCGATGcc               <  2:82132/140‑1 (MQ=255)

                                                                                                                      cccGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGCGATGcc               <  1:387946/140‑1 (MQ=255)

                                                                                                                       ccGAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGCGATGCCa              >  2:1045295/1‑140 (MQ=255)

                                                                                                                         gAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCTGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGCGATGCCAac            >  2:1120419/1‑140 (MQ=255)

                                                                                                                         gAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGCGATGCCAac            >  2:231843/1‑140 (MQ=255)

                                                                                                                         gAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGCGATGCCAac            >  2:1201265/1‑140 (MQ=255)

                                                                                                                         gAATGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGCGATGCCAac            >  1:789328/1‑140 (MQ=255)

                                                                                                                         gAATGGGTGAAGTGGCTGCGGATGACGGTACGGAGTTGCCTATAGCCGCCACCTTTGCCGGTGCACATTCGCTGACGGTCGATGCAGTCATTGTCCCCTACGGCAAGCTCGCGGAAAACGCTGACAACGGCGATTCCAac            >  2:1491261/1‑140 (MQ=255)

                                                                                                                          aaTGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGCGATGCCAact           <  1:1460026/140‑1 (MQ=255)

                                                                                                                           aTGGGTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGCGATGCCAacta          <  2:1226414/140‑1 (MQ=255)

                                                                                                                              ggTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGCGATGCCAActacta       >  1:364483/1‑140 (MQ=255)

                                                                                                                               gTGAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGCGATGCCAACtactac      >  2:314496/1‑140 (MQ=255)

                                                                                                                                 gAAGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGCGATGCCAACTACTACCt    >  2:621078/1‑140 (MQ=255)

                                                                                                                                   aGTGGCTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGCGATGCCAACTACTACCTGa  >  1:632547/1‑140 (MQ=255)

GGTGATGTGAAAGGTCGCGTGGTAGCGATTTTACTTAATGATGAAGTGAGATCGGCAGACCTTCTGGCCATTCTCAAGGCGCTGAAGGCCAAAGGCGTTCATGCCAAACTGCTCTACTCCCGAATGGGTGAAGTGACTGCGGATGACGGTACGGTGTTGCCTATAGCCGCTACCTTTGCCGGTGCACCTTCGCTGACGGTCGATGCGGTCATTGTCCCTTGCGGCAATATCGCGGATATCGCTGACAACGGCGATGCCAACTACTACCTGA  >  NC_000913/1815652‑1815922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A