Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	124	90.1	3003320	99.2%	2979293	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,218,942	T→C	K180K (AAA→AAG)	osmF ←	glycine betaine ABC transporter periplasmic binding protein OsmF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,218,942	0	T	C	100.0%	189.6 / NA	51	K180K (AAA→AAG)	osmF	glycine betaine ABC transporter periplasmic binding protein OsmF
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (22/29); total (22/29)

ACCGGGCCGTCAGTGCCGTAAGCCATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGA  >  NC_000913/2218808‑2219077

aCCGGGCCGTCAGTGCCGTAAGCCATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTc                                                                                                                                    >  2:597137/1‑140 (MQ=255)

                    aGCCATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCgg                                                                                                                <  1:155407/140‑1 (MQ=255)

                       cATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGgcgc                                                                                                             >  1:35980/1‑140 (MQ=255)

                       cATGGCAGCTTTAACGCCAGAGGTTTGCGGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGgcgc                                                                                                             <  1:1332853/140‑1 (MQ=255)

                        aTTGCAGCATTACCGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGcgcg                                                                                                            <  2:1378009/140‑1 (MQ=255)

                        aTTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGcgcg                                                                                                            <  2:728012/140‑1 (MQ=255)

                         ttGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGt                                                                                                           <  2:1168206/140‑1 (MQ=255)

                         ttGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGt                                                                                                           >  1:910660/1‑140 (MQ=255)

                         ttGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGt                                                                                                           >  1:161015/1‑140 (MQ=255)

                                    aCGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAActc                                                                                                >  1:46553/1‑140 (MQ=255)

                                      gCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTg                                                                                              <  2:1349248/140‑1 (MQ=255)

                                         agagGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCg                                                                                           >  2:1349704/1‑140 (MQ=255)

                                           agGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAg                                                                                         >  1:1448037/1‑140 (MQ=255)

                                                tGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGc                                                                                    >  1:1495431/1‑140 (MQ=255)

                                                           ggCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAgg                                                                         <  2:574424/140‑1 (MQ=255)

                                                            gCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGt                                                                        >  2:784644/1‑140 (MQ=255)

                                                             cTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTg                                                                       <  2:264657/140‑1 (MQ=255)

                                                               ttGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTgcc                                                                     >  1:949494/1‑140 (MQ=255)

                                                                tgttCGTCACCGCCGTGTCGCCCCCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTgccg                                                                    <  2:861894/137‑1 (MQ=255)

                                                                  agcGTCACCGAAGTGTCGCCTCCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATCGGCCTTTTCAAACTCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGGgccgcc                                                                  <  2:1293169/138‑1 (MQ=255)

                                                                  aTCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTgccgcc                                                                  <  2:46553/140‑1 (MQ=255)

                                                                  aTCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTgccgcc                                                                  <  2:582698/140‑1 (MQ=255)

                                                                  aTCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTgccgcc                                                                  <  2:34082/140‑1 (MQ=255)

                                                                  aTCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTgccgcc                                                                  <  1:288482/140‑1 (MQ=255)

                                                                  aTCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTgccgcc                                                                  <  1:1198892/140‑1 (MQ=255)

                                                                     gTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCctc                                                               <  1:597137/140‑1 (MQ=255)

                                                                     gTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCctc                                                               <  2:1448037/140‑1 (MQ=255)

                                                                     gTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCctc                                                               <  2:647413/140‑1 (MQ=255)

                                                                        aCCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTg                                                            <  1:384631/140‑1 (MQ=255)

                                                                          cgccgTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCa                                                          >  1:895150/1‑140 (MQ=255)

                                                                          cgccgTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCa                                                          <  2:508980/140‑1 (MQ=255)

                                                                            ccgTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGa                                                        >  2:1139370/1‑140 (MQ=255)

                                                                                 tCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACg                                                   >  2:387355/1‑140 (MQ=255)

                                                                                 tCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACg                                                   >  1:633631/1‑140 (MQ=255)

                                                                                  cGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGa                                                  <  1:379311/140‑1 (MQ=255)

                                                                                    ccaccaGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACt                                                <  1:955865/140‑1 (MQ=255)

                                                                                      accaGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCa                                              >  1:489393/1‑140 (MQ=255)

                                                                                         agccagTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGt                                           >  2:1486549/1‑140 (MQ=255)

                                                                                            cagTGACAGCAACTGATCCTGACCGAACTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCgg                                        <  2:329389/140‑1 (MQ=255)

                                                                                                   aGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGa                                 >  1:420049/1‑140 (MQ=255)

                                                                                                     cAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAg                               >  1:149175/1‑140 (MQ=255)

                                                                                                     cAACTGATCCTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAg                               >  2:622358/1‑140 (MQ=255)

                                                                                                             ccTGACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTa                       >  2:127856/1‑140 (MQ=255)

                                                                                                                gACCGAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTAttt                    >  1:985734/1‑140 (MQ=255)

                                                                                                                    gAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTtct                <  1:45039/140‑1 (MQ=255)

                                                                                                                    gAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTtct                <  2:1495431/140‑1 (MQ=255)

                                                                                                                    gAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTtct                <  2:1006407/140‑1 (MQ=255)

                                                                                                                    gAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTtct                <  2:128080/140‑1 (MQ=255)

                                                                                                                    gAGCTTAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTtct                <  1:1349704/140‑1 (MQ=255)

                                                                                                                         tAAAGCCATAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGcc           <  1:1486549/140‑1 (MQ=255)

                                                                                                                                aTAGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCt    >  1:758706/1‑140 (MQ=255)

                                                                                                                                  aGGCCTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGa  <  2:910660/140‑1 (MQ=255)

ACCGGGCCGTCAGTGCCGTAAGCCATTGCAGCATTAACGCCAGAGGTTTGCTGGGCAGCGGCTTTGATCGTCACCGCCGTGTCGCCACCAGCCAGTGACAGCAACTGATCCTGACCGAGCTTAAAGCCATAGGCTTTTTCAAACGCGGGTAACGCATCGGCGCGTTCGATAAACTCTGCCGAGGCTGCCAGTTTGAAGGTGCCGCCCTCTTGCAGATAACGACTCAGGTCGGCAAGCGAAGTGAGTTTATTTTTCTCTGCCACATCCTGA  >  NC_000913/2218808‑2219077

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A