Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	124	90.1	3003320	99.2%	2979293	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,208,729	T→C	F236L (TTC→CTC)	ttdT →	L‑tartrate:succinate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,208,729	0	T	C	100.0%	189.5 / NA	51	F236L (TTC→CTC)	ttdT	L‑tartrate:succinate antiporter
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (33/18); total (33/18)

GCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGC  >  NC_000913/3208592‑3208863

gCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTc                                                                                                                                      >  1:484854/1‑140 (MQ=255)

  tCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCt                                                                                                                                    <  2:680937/140‑1 (MQ=255)

     tACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCCTTTTCTTGACGGCGATGGCACCCAAATTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTTCTCgg                                                                                                                                 >  2:1276640/1‑140 (MQ=255)

     tACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCCAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGGGACTGGCTCCTCgg                                                                                                                                 >  1:195704/1‑140 (MQ=255)

     tACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCgg                                                                                                                                 >  1:1301500/1‑140 (MQ=255)

       catcatGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGa                                                                                                                               <  1:1078829/140‑1 (MQ=255)

          catGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGt                                                                                                                            >  2:1132322/1‑140 (MQ=255)

            tgtgGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTg                                                                                                                          >  1:85813/1‑140 (MQ=255)

            tgtgGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTg                                                                                                                          <  2:1097153/140‑1 (MQ=255)

              tgGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATAGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGcc                                                                                                                        >  2:458402/1‑140 (MQ=255)

               ggATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCg                                                                                                                       <  2:75365/140‑1 (MQ=255)

                gATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGc                                                                                                                      >  1:1050946/1‑140 (MQ=255)

                gATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGc                                                                                                                      >  2:736486/1‑140 (MQ=255)

                gATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGc                                                                                                                      >  2:736487/1‑140 (MQ=255)

                 aTGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCt                                                                                                                     <  2:715463/140‑1 (MQ=255)

                 aTGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCt                                                                                                                     <  2:557697/140‑1 (MQ=255)

                 aTGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCt                                                                                                                     >  1:557864/1‑140 (MQ=255)

                    gggATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAg                                                                                                                  >  1:1478424/1‑140 (MQ=255)

                    gggATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAg                                                                                                                  >  1:47141/1‑140 (MQ=255)

                      gATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCa                                                                                                                >  2:1269196/1‑140 (MQ=255)

                       aTTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCAt                                                                                                               >  1:1410442/1‑140 (MQ=255)

                           ttgCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTa                                                                                                           >  1:1445069/1‑140 (MQ=255)

                           ttgCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTa                                                                                                           >  1:365766/1‑140 (MQ=255)

                               cGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTgg                                                                                                       >  1:818202/1‑140 (MQ=255)

                                     cGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTctgc                                                                                                 >  1:656061/1‑140 (MQ=255)

                                     cGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTctgc                                                                                                 >  1:1001455/1‑140 (MQ=255)

                                      gTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTctgct                                                                                                <  1:1485984/140‑1 (MQ=255)

                                       tGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTctgctg                                                                                               <  1:551429/140‑1 (MQ=255)

                                               gcCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAACAGCGCATCTCAGGCCACGCTGAGTTGGGGCGACTGGCTACTCGGGATGATGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCctg                                                                                       >  1:799881/1‑140 (MQ=255)

                                                ccATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCctgg                                                                                      <  2:1335515/140‑1 (MQ=255)

                                                ccATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCctgg                                                                                      <  2:557864/140‑1 (MQ=255)

                                                    tttCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCctggctgg                                                                                  <  2:1050946/140‑1 (MQ=255)

                                                       cTTGACGGCGATGGCACTAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCTCGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCtt                                                                               <  2:1450873/140‑1 (MQ=255)

                                                       cTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCtt                                                                               <  2:1301500/140‑1 (MQ=255)

                                                        ttGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTa                                                                              <  2:795866/140‑1 (MQ=255)

                                                          gACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACg                                                                            >  1:25919/1‑140 (MQ=255)

                                                           aCGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGt                                                                           >  2:1256603/1‑140 (MQ=255)

                                                            cGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTg                                                                          <  2:47141/140‑1 (MQ=255)

                                                            cGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTg                                                                          <  1:1269196/140‑1 (MQ=255)

                                                            cGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTg                                                                          <  2:1070532/140‑1 (MQ=255)

                                                            cGGCGATAGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTg                                                                          <  1:458402/140‑1 (MQ=255)

                                                               cGAGGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTGGTGTCGACTGGCTCCTCGGGCTGTTGCCGCTGAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTg                                                                       >  2:781466/1‑140 (MQ=255)

                                                                  tGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTAc                                                                    >  1:356030/1‑140 (MQ=255)

                                                                                     aaTTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTg                                                 >  1:175282/1‑140 (MQ=255)

                                                                                       ttGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCCGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGc                                               >  2:702892/1‑140 (MQ=255)

                                                                                                         cATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTgg                             >  1:435832/1‑140 (MQ=255)

                                                                                                         cATCTCACGCCACGCTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTgg                             >  2:1205679/1‑140 (MQ=255)

                                                                                                                      gcTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGaat                >  1:890322/1‑139 (MQ=255)

                                                                                                                      gcTGAGTTGGGGCGACTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAAc                >  1:1166154/1‑140 (MQ=255)

                                                                                                                                    ccTGGCTCCTCGGGATGCTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGCACTGAAGCCTGGCGAACAGGGGCCGCGCCGGGCAGCGACGGAACTGCAGGCAATGGGc  >  2:570949/2‑140 (MQ=255)

                                                                                                                                    aCTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGc  >  1:273144/1‑140 (MQ=255)

                                                                                                                                    aCTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGc  >  1:546557/1‑140 (MQ=255)

                                                                                                                                    aCTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGc  >  1:454336/1‑140 (MQ=255)

                                                                                                                                    aCTGGCTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAACGGGc  >  1:114784/1‑140 (MQ=255)

GCTCGTACATCATGTGGATGGGGATTGTTGCCGACTGCGTGACCAGCGCCATTTTCTTGACGGCGATGGCACCAAACTTGCTGTTAATTGGACTGATGAAAAGCGCATCTCACGCCACGCTGAGTTGGGGCGACTGGTTCCTCGGGATGTTGCCGCTCAGCATTTTACTGGTTCTGCTGGTTCCCTGGCTGGCTTACGTGCTGTACCCGCCGGTACTGAAGTCTGGCGATCAGGTGCCGCGCTGGGCAGAGACGGAACTGCAGGCAATGGGC  >  NC_000913/3208592‑3208863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A