Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F28 I1 R1	124	90.1	3003320	99.2%	2979293	139.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,856,300	Δ1 bp	coding (8/348 nt)	yidH ←	conserved inner membrane protein YidH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,856,298	0	A	.	94.5%	350.5 / 18.0	91	coding (10/348 nt)	yidH	conserved inner membrane protein YidH
Reads supporting (aligned to +/- strand): ref base A (0/5); new base . (32/54); total (32/59)
Fisher's exact test for biased strand distribution p-value = 1.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.40e-02

ACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATA  >  NC_000913/3856159‑3856436

aCGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGa                                                                                                                                             <  1:912586/140‑1 (MQ=255)

     cGTGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAtttt                                                                                                                                        <  1:1117492/140‑1 (MQ=255)

     cGTGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAtttt                                                                                                                                        <  1:865804/140‑1 (MQ=255)

      gTGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGTCGCTTCTCCGAGGCGGGAAttttc                                                                                                                                       <  2:1398793/140‑2 (MQ=255)

      gTGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAttttc                                                                                                                                       <  1:319918/140‑2 (MQ=255)

      gTGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAttttc                                                                                                                                       <  1:253341/140‑2 (MQ=255)

      gTGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTc                                                                                                                                      <  1:951146/140‑1 (MQ=255)

      gTGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTc                                                                                                                                      <  2:776956/140‑1 (MQ=255)

       tGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCa                                                                                                                                     >  1:922668/1‑140 (MQ=255)

       tGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCa                                                                                                                                     >  1:907172/1‑140 (MQ=255)

       tGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCa                                                                                                                                     >  1:1125253/1‑140 (MQ=255)

       tGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCa                                                                                                                                     <  1:16991/140‑1 (MQ=255)

        ggCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCAt                                                                                                                                    <  2:1284233/140‑1 (MQ=255)

             aaTCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCAttgttg                                                                                                                               <  1:694596/140‑1 (MQ=255)

              aTCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGc                                                                                                                              >  1:1184251/1‑140 (MQ=255)

              aTCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGc                                                                                                                              >  1:8864/1‑140 (MQ=255)

               tCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCt                                                                                                                             <  1:772232/140‑1 (MQ=255)

                   gcgcAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCtt                                                                                                                         <  1:1252265/140‑1 (MQ=255)

                    cgcAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCttt                                                                                                                        >  1:822884/1‑140 (MQ=255)

                             aTCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCa                                                                                                               >  2:142726/1‑140 (MQ=255)

                             aTCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCa                                                                                                               >  2:278104/1‑140 (MQ=255)

                               cAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACt                                                                                                             <  1:722000/140‑1 (MQ=255)

                               cAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACt                                                                                                             <  1:843186/140‑1 (MQ=255)

                                aaGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTa                                                                                                            <  1:1288889/140‑1 (MQ=255)

                                  gCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAAc                                                                                                          <  2:345188/140‑1 (MQ=255)

                                   cccAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑GATTTTCATTGTTGCTCCTTTGCAATTTTCCTAAcc                                                                                                         >  2:1350685/1‑139 (MQ=255)

                                   cccAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACg                                                                                                         >  1:731991/1‑140 (MQ=255)

                                   cccAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACg                                                                                                         >  1:1190074/1‑140 (MQ=255)

                                       aCCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGctct                                                                                                     <  2:602865/140‑1 (MQ=255)

                                       aCCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGctct                                                                                                     <  1:785366/140‑1 (MQ=255)

                                       aCCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGctct                                                                                                     >  1:646552/1‑140 (MQ=255)

                                       aCCCCCGCCACCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGctct                                                                                                     <  1:191113/140‑1 (MQ=255)

                                        cccccGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTa                                                                                                    >  2:437346/1‑140 (MQ=255)

                                            cgccgcCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCCTTGTTGCTCCTTTGCCATTTCACTAACGCCCTACCtt                                                                                                >  2:1144762/1‑140 (MQ=255)

                                                   aGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑t                                                                                        >  2:144337/1‑140 (MQ=255)

                                                                   tACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑CATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTTGCGG‑GTTTTTTCAGAATTTCg                                                                        >  2:1021363/1‑140 (MQ=255)

                                                                   tACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCg                                                                        >  2:369111/1‑140 (MQ=255)

                                                                    aCGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGt                                                                       >  2:221249/1‑140 (MQ=255)

                                                                    aCGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGt                                                                       >  1:942848/1‑140 (MQ=255)

                                                                     cGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTc                                                                      <  1:211974/140‑1 (MQ=255)

                                                                     cGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTc                                                                      <  2:822884/140‑1 (MQ=255)

                                                                        aTCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑GATTTTCATTGTTGCTCCCTTGCCATTTCACTAACGCCCTACCCTTAGCGG‑GTTTTTTTAGAATTTCGTCCGc                                                                   >  2:983067/1‑140 (MQ=255)

                                                                        aTCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGc                                                                   >  2:1327079/1‑140 (MQ=255)

                                                                         tCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGcc                                                                  <  2:731991/140‑1 (MQ=255)

                                                                          ccACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGccc                                                                 <  2:801190/140‑1 (MQ=255)

                                                                           cACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCg                                                                >  2:24948/1‑140 (MQ=255)

                                                                           cACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCg                                                                <  1:805094/140‑1 (MQ=255)

                                                                             cGCCAGAAAAGTACGCTCATTAGCCAGTGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGtt                                                              >  1:1238481/1‑140 (MQ=255)

                                                                             cGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTTATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGtt                                                              >  1:424396/1‑140 (MQ=255)

                                                                             cGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGtt                                                              >  2:152964/1‑140 (MQ=255)

                                                                             cGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGtt                                                              >  2:1187084/1‑140 (MQ=255)

                                                                             cGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGtt                                                              >  1:1064415/1‑140 (MQ=255)

                                                                             cGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGtt                                                              <  1:41596/140‑1 (MQ=255)

                                                                               ccAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTc                                                            <  1:933207/140‑1 (MQ=255)

                                                                               ccAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTc                                                            <  2:628904/140‑1 (MQ=255)

                                                                                  gAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTTATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑GTTTTTTAAGAATTTCGTCCGCCCGTTTCCTg                                                         >  2:546518/1‑140 (MQ=255)

                                                                                    aaaGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGat                                                       <  2:264982/140‑1 (MQ=255)

                                                                                    aaaGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGat                                                       <  2:1376205/140‑1 (MQ=255)

                                                                                     aaGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGata                                                      <  1:469217/140‑1 (MQ=255)

                                                                                        tACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGc                                                   >  1:698591/1‑140 (MQ=255)

                                                                                             tCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTg                                              <  2:907172/140‑1 (MQ=255)

                                                                                               aTTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGcc                                            <  1:1190483/140‑1 (MQ=255)

                                                                                                 tAGCCAGCGAGGAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑GATTTTCCTTGTTGCTTCTTTGCAATTTCACTAACGCTCTTCCTTTAGGCG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCAt                                          <  2:1229001/140‑1 (MQ=255)

                                                                                                 tAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTTCCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCAt                                          <  1:409725/140‑1 (MQ=255)

                                                                                                 tAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAGTTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCAt                                          <  1:1351217/140‑1 (MQ=255)

                                                                                                 tAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCAt                                          <  1:1157392/140‑1 (MQ=255)

                                                                                                 tAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCAt                                          <  2:646552/140‑1 (MQ=255)

                                                                                                 tAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCAt                                          <  2:1064415/140‑1 (MQ=255)

                                                                                                  aGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATc                                         <  2:8864/140‑1 (MQ=255)

                                                                                                      ggCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCt                                     <  2:278722/139‑1 (MQ=255)

                                                                                                      aGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCt                                     <  1:151541/140‑1 (MQ=255)

                                                                                                      aGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCt                                     <  2:286326/140‑1 (MQ=255)

                                                                                                      aGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCt                                     <  2:942848/140‑1 (MQ=255)

                                                                                                         gagaAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACa                                  <  1:1350685/140‑1 (MQ=255)

                                                                                                           gaAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAag                                <  1:437346/140‑1 (MQ=255)

                                                                                                           gaAGCGGTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCCTTATCGG‑TTTTTTTTAGAATTTCGTCCGCCCGGTTCCTGATATGCTTTTGCCATCCGCTACAag                                >  2:139816/1‑140 (MQ=255)

                                                                                                                ggTAATCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGt                           <  1:1144762/140‑1 (MQ=255)

                                                                                                                   aaTCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGATGTCAg                        <  1:24948/140‑1 (MQ=255)

                                                                                                                   aaTCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAg                        <  2:494417/140‑1 (MQ=255)

                                                                                                                   aaTCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAg                        <  2:1203192/140‑1 (MQ=255)

                                                                                                                   aaTCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAg                        <  2:1238115/140‑1 (MQ=255)

                                                                                                                   aaTCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAg                        <  2:922668/140‑1 (MQ=255)

                                                                                                                   aaTCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAg                        <  1:428305/140‑1 (MQ=255)

                                                                                                                   aaTCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAg                        <  1:1327079/140‑1 (MQ=255)

                                                                                                                   aaTCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAg                        <  2:424396/140‑1 (MQ=255)

                                                                                                                   aaTCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAg                        <  1:369111/140‑1 (MQ=255)

                                                                                                                   aaTCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAg                        <  1:221249/140‑1 (MQ=255)

                                                                                                                   aaTCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAg                        <  1:152964/140‑1 (MQ=255)

                                                                                                                   aaTCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAg                        <  1:207352/140‑1 (MQ=255)

                                                                                                                   aaTCAGGCGCTTCTCCGAGG‑GGGGTATTTTTAATGTTTTTCCTTTTGAATTTCACTAATGCTATTCCTTTCACGG‑GTTTTTTTATAATTTTGTTCGGCCCATTCCTGATTTTCTTTTGCCATACGCTAAAGAGAGGTctt                        >  2:1012186/1‑138 (MQ=255)

                                                                                                                    aTCAGGCGCTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGa                       >  2:1063774/1‑140 (MQ=255)

                                                                                                                          cgcTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTa                 >  2:281182/1‑140 (MQ=255)

                                                                                                                          cgcTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTa                 >  2:1208009/1‑140 (MQ=255)

                                                                                                                            cTTCTCCGAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATc               >  1:1056912/1‑140 (MQ=255)

                                                                                                                                   gAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTc        <  1:1188873/140‑1 (MQ=255)

                                                                                                                                   gAGGCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTc        <  1:142726/140‑1 (MQ=255)

                                                                                                                                      gCGGG‑AATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGGTTTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCaa      >  2:548756/1‑140 (MQ=255)

                                                                                                                                         ggAAA‑TTTCATTGTTGCTCCTTTTCAATTTCACTAACGCTCTCCCTTTAGCGG‑TTTTTTTAAGAAATTCGTCCACCCGTTTCCCGATATGCTTTTGCCATCCGCTACAAGGAGGGCAGGTGGGTATCATAGGTCaaaata  >  2:719385/1‑140 (MQ=255)

ACGGGCGTGGCGAAATCTGGCGCAAGTTGATCAAGCCCAACCCCCGCCGCCAGAAATCCTAGTGCAGTACGGATCCACGCCAGAAAAGTACGCTCATTAGCCAGCGAGAAGCGGTAATCAGGCGCTTCTCCGAGGCGGGAAATTTTCATTGTTGCTCCTTTGCAATTTCACTAACGCTCTACCTTTAGCGG‑TTTTTTTAAGAATTTCGTCCGCCCGTTTCCTGATATGCTTTTGCCATCCGCTACAAGGAGGTCAGATGGGTATCATAGCTCAAAATA  >  NC_000913/3856159‑3856436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A