Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	796,417	T→C	Y186H (TAT→CAT)	modB →	molybdate ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	796,417	0	T	C	100.0%	119.9 / NA	33	Y186H (TAT→CAT)	modB	molybdate ABC transporter membrane subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/14); total (19/14)

CGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGG  >  NC_000913/796283‑796543

cGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATAcc                                                                                                                           <  1:192808/140‑1 (MQ=255)

cGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATAcc                                                                                                                           >  1:242902/1‑140 (MQ=255)

       gCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATcc                                                                                                                    <  1:289619/140‑1 (MQ=255)

           aCCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGAc                                                                                                                >  2:971767/1‑140 (MQ=255)

           aCCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGAc                                                                                                                >  2:251350/1‑140 (MQ=255)

                             gttgGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTgg                                                                                              <  2:187174/140‑1 (MQ=255)

                              ttgGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTggg                                                                                             >  1:114828/1‑139 (MQ=255)

                                ggtacggtacTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAgc                                                                                           >  2:101069/1‑140 (MQ=255)

                                    cggtacTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAgcggcg                                                                                       <  2:252211/140‑1 (MQ=255)

                                       tacTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCgaga                                                                                    >  1:857674/1‑140 (MQ=255)

                                           ggCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTgg                                                                                              >  2:133123/1‑126 (MQ=255)

                                           ggCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTgg                                                                                              <  1:133123/126‑1 (MQ=255)

                                            gCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACtgtg                                                                               >  1:769707/1‑140 (MQ=255)

                                             cTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGc                                                                              <  2:813261/140‑1 (MQ=255)

                                                 tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGTCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCatta                                                                          >  2:459738/1‑140 (MQ=255)

                                                 tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCcttt                                                                          >  1:133814/1‑139 (MQ=255)

                                                 tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCatta                                                                          >  1:617064/1‑140 (MQ=255)

                                                 tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCatta                                                                          >  2:9595/1‑140 (MQ=255)

                                                 tGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCatta                                                                          >  1:359010/1‑140 (MQ=255)

                                                                 gAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGc                                                          <  1:908780/140‑1 (MQ=255)

                                                                 gAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGc                                                          <  1:168982/140‑1 (MQ=255)

                                                                              ccATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTg                                             >  1:49229/1‑140 (MQ=255)

                                                                              ccATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTg                                             >  1:792981/1‑140 (MQ=255)

                                                                                   aCCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGAt                                        <  1:101069/140‑1 (MQ=255)

                                                                                            tCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAAtg                               >  1:467811/1‑140 (MQ=255)

                                                                                               aaCATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAAtggct                            >  1:971875/1‑140 (MQ=255)

                                                                                                       tGGTGAAACGCGAACCATTCCTTCTGTCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGaa                    <  1:459738/140‑1 (MQ=255)

                                                                                                           gAAACGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAg                <  2:617064/140‑1 (MQ=255)

                                                                                                            aaaCGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGc               >  1:663429/1‑140 (MQ=255)

                                                                                                              aCGCGAACCATTCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCg             >  1:532325/1‑140 (MQ=255)

                                                                                                                        ttCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAAcgggcg   <  1:9595/140‑1 (MQ=255)

                                                                                                                        ttCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAAcgggcg   <  2:792981/140‑1 (MQ=255)

                                                                                                                         tCCTTCTGCCATGCATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAAcgggcgg  <  1:251350/140‑1 (MQ=255)

CGTTACCGCTGACCTTACCGGGAATTATTGTTGGTACGGTACTGGCTTTTGCTCGTTCTCTCGGTGAGTTTGGTGCAACCATCACCTTTGTGTCGAACATTCCTGGTGAAACGCGAACCATTCCTTCTGCCATGTATACCCTGATCCAGACCCCCGGCGGGGAAAGTGGAGCGGCGAGACTGTGCATTATTTCTATTGCGCTGGCGATGATCTCCCTGTTGATTTCAGAATGGCTGGCCAGAATCAGCCGTGAACGGGCGG  >  NC_000913/796283‑796543

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A