Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	927,208	G→A	S75S (AGC→AGT)	aat ←	leucyl/phenylalanyl‑tRNA‑‑protein transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	927,208	0	G	A	100.0%	167.6 / NA	50	S75S (AGC→AGT)	aat	leucyl/phenylalanyl‑tRNA‑‑protein transferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/30); total (20/30)

GGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCT  >  NC_000913/927072‑927333

ggTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTg                                                                                                                            <  2:353291/140‑1 (MQ=255)

   aGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGata                                                                                                                         <  1:109657/140‑1 (MQ=255)

        tCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAg                                                                                                                    <  2:913498/140‑1 (MQ=255)

             cacgccacgcGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGAtt                                                                                                               <  2:738641/140‑1 (MQ=255)

             cacgccacgcGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGAtt                                                                                                               <  1:200054/140‑1 (MQ=255)

             cacgccacgcGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGAtt                                                                                                               <  1:767098/140‑1 (MQ=255)

             cacgccacgcGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGAtt                                                                                                               <  1:745279/140‑1 (MQ=255)

                      cgTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATa                                                                                                      >  1:114802/1‑140 (MQ=255)

                         gATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCa                                                                                                   <  1:792351/140‑1 (MQ=255)

                          aTCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCAc                                                                                                  >  2:983473/1‑140 (MQ=255)

                          aTCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCAc                                                                                                  >  2:852447/1‑140 (MQ=255)

                          aTCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCAc                                                                                                  <  1:183901/140‑1 (MQ=255)

                                   ccTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCgggg                                                                                         >  2:653369/1‑140 (MQ=255)

                                     ttcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGAt                                                                                       >  1:324899/1‑140 (MQ=255)

                                     ttcttcGCGATCGCTGGCACAGCATTCAATGACATGTCCAAAAGCGTAATCCATCGTGACACGATAGGGAGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACTGCGCGGGGAt                                                                                       <  2:59203/140‑1 (MQ=255)

                                      tcttcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATc                                                                                      <  2:367457/140‑1 (MQ=255)

                                         tcGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCggg                                                                                   <  2:496052/140‑1 (MQ=255)

                                          cgcgATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGc                                                                                  >  1:267300/1‑140 (MQ=255)

                                          cgcgATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGAGc                                                                                  >  1:59203/1‑140 (MQ=255)

                                                 gCTGGCACAGCCTTCAATGTCCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGTGATCGGGCGaccacc                                                                           <  2:820026/140‑1 (MQ=255)

                                                       acaGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGACCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGa                                                                     >  2:517691/1‑140 (MQ=255)

                                                         aGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATg                                                                   <  2:167137/140‑1 (MQ=255)

                                                           ccTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGTTCGGGCGACCACCAGAGGATggg                                                                 >  1:420889/1‑140 (MQ=255)

                                                           ccTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATggg                                                                 <  2:429841/140‑1 (MQ=255)

                                                           ccTTCAAAGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATggg                                                                 >  2:209285/1‑140 (MQ=255)

                                                            cTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATgggg                                                                >  2:11556/1‑140 (MQ=255)

                                                                   gacctgaccAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTg                                                         >  1:961293/1‑140 (MQ=255)

                                                                     cctgaccAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGga                                                       >  1:319895/1‑140 (MQ=255)

                                                                              aaGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCAc                                              <  1:188593/140‑1 (MQ=255)

                                                                                                 acGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGt                           <  2:684014/140‑1 (MQ=255)

                                                                                                     tAGGGCGAGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGc                       >  1:456958/1‑140 (MQ=255)

                                                                                                            aGCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAAc                >  1:564889/1‑140 (MQ=255)

                                                                                                             gCGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACa               >  2:739790/1‑140 (MQ=255)

                                                                                                              cGTTTATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAg              >  2:1022578/1‑140 (MQ=255)

                                                                                                                  tATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgc          >  1:32926/1‑140 (MQ=255)

                                                                                                                  tATGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgc          >  1:979596/1‑140 (MQ=255)

                                                                                                                   aTGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcg         <  2:44642/140‑1 (MQ=255)

                                                                                                                   aTGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcg         <  2:324899/140‑1 (MQ=255)

                                                                                                                   aTGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcg         <  1:983473/140‑1 (MQ=255)

                                                                                                                   aTGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcg         <  2:930271/140‑1 (MQ=255)

                                                                                                                   aTGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcg         <  1:852447/140‑1 (MQ=255)

                                                                                                                    tGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACTGgcgcgc        <  2:285726/140‑1 (MQ=255)

                                                                                                                    tGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc        <  1:209285/140‑1 (MQ=255)

                                                                                                                    tGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc        <  2:744657/140‑1 (MQ=255)

                                                                                                                    tGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc        <  1:644523/140‑1 (MQ=255)

                                                                                                                    tGAAATCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGgcgcgc        <  1:11556/140‑1 (MQ=255)

                                                                                                                         tCGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGc   >  1:769903/1‑140 (MQ=255)

                                                                                                                          cGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATGGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCt  <  1:1022578/140‑1 (MQ=255)

                                                                                                                          cGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCt  <  1:957906/140‑1 (MQ=255)

                                                                                                                          cGCTTCATACTACGACTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCt  <  1:338149/140‑1 (MQ=255)

GGTAGGCTTCGACCACGCCACGCGTGATCCAGGTTCCTTCTTCGCGATCGCTGGCACAGCCTTCAATGACCTGACCAAAAGCGTAATTCATCGTGACACGATAGGGCGAGCGTTTATGAAATCGCTTCATACTACGGCTGATATGCAGTGATTCTGGCCATAGCACCGCGCGGGGATCGGGCGACCACCAGAGGATGGGGTCGCCTGGAGAAAACCACGGAAAAATACCACGCTGGTAAGCCATTAACAGGCGCGCAGGGCT  >  NC_000913/927072‑927333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A