Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,040,030	A→G	K245K (AAA→AAG)	appB →	cytochrome bd‑II ubiquinol oxidase subunit II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,040,030	0	A	G	100.0%	162.0 / NA	44	K245K (AAA→AAG)	appB	cytochrome bd‑II ubiquinol oxidase subunit II
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (26/18); total (26/18)

AGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGG  >  NC_000913/1039897‑1040163

ggCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCACAGGATGATAACGGACCTTCCAATCCGTTAATGAAGCtggtg                                                                                                                                 <  2:406963/139‑1 (MQ=255)

aGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCtggtg                                                                                                                                 <  2:661849/140‑1 (MQ=255)

  cTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGc                                                                                                                               <  1:873216/140‑1 (MQ=255)

   tGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCa                                                                                                                              <  1:823214/140‑1 (MQ=255)

      cGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTg                                                                                                                           <  1:279683/140‑1 (MQ=255)

        aCCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCt                                                                                                                         <  2:168956/140‑1 (MQ=255)

                    gCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTg                                                                                                             <  2:833917/140‑1 (MQ=255)

                     cACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTgg                                                                                                            <  2:609100/140‑1 (MQ=255)

                          ttGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGGCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGGGCCTGGATGaa                                                                                                       >  2:415845/1‑140 (MQ=255)

                                  gCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGGCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGGGCCTGGATGAATAATTTTg                                                                                               >  1:48652/1‑140 (MQ=255)

                                    tgtgCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTc                                                                                             >  1:952904/1‑140 (MQ=255)

                                    tgtgCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGGGCCTGGATGAATAATTTTGTc                                                                                             >  2:341494/1‑140 (MQ=255)

                                    tgtgCTTTTTGCTGGCGGGTTACTGGCTGTGGGGCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTc                                                                                             >  2:417528/1‑140 (MQ=255)

                                    tgtgCTTTTTGCTGGCGGGTTACTGGCTGTGGGGCGGGATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCCGTGCTACCTGGGGCCTGGATGAATAATTTTGTc                                                                                             >  2:108657/1‑140 (MQ=255)

                                              gCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCg                                                                                   <  2:278877/140‑1 (MQ=255)

                                              gCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCg                                                                                   <  2:962033/140‑1 (MQ=255)

                                                 ggCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGttt                                                                                <  1:411282/140‑1 (MQ=255)

                                                               tgtgGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCg                                                                  <  2:818833/140‑1 (MQ=255)

                                                               tgtgGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCg                                                                  <  2:333837/140‑1 (MQ=255)

                                                                        gTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGa                                                         <  1:922063/140‑1 (MQ=255)

                                                                        gTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGa                                                         <  2:554365/140‑1 (MQ=255)

                                                                          aTTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAtt                                                       <  2:1014495/140‑1 (MQ=255)

                                                                              aTGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAttcttc                                                   >  2:206667/1‑140 (MQ=255)

                                                                              aTGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGAttcttc                                                   >  2:495167/1‑140 (MQ=255)

                                                                                   tttGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGccc                                              >  2:888345/1‑140 (MQ=255)

                                                                                   tttGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGccc                                              >  1:419729/1‑140 (MQ=255)

                                                                                   tttGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTAGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGccc                                              >  2:493124/1‑140 (MQ=255)

                                                                                     tGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCAt                                            >  2:734962/1‑140 (MQ=255)

                                                                                         cTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCt                                        <  1:417528/140‑1 (MQ=255)

                                                                                            cTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGTTTCTTCTGCCCATTGCTGAc                                     >  1:22379/1‑140 (MQ=255)

                                                                                            cTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGAc                                     >  2:182110/1‑140 (MQ=255)

                                                                                            cTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGAc                                     >  1:997722/1‑140 (MQ=255)

                                                                                            cTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGAc                                     >  1:808638/1‑140 (MQ=255)

                                                                                            cTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGAc                                     >  1:448780/1‑140 (MQ=255)

                                                                                            cTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGAc                                     >  2:428523/1‑140 (MQ=255)

                                                                                            cTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGAc                                     >  2:43478/1‑140 (MQ=255)

                                                                                                 ccAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGa                                >  1:256925/1‑140 (MQ=255)

                                                                                                 ccAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGa                                >  1:788367/1‑140 (MQ=255)

                                                                                                    ggATGCTAACGGTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATgg                             >  2:941916/1‑140 (MQ=255)

                                                                                                               gTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGt                  >  2:190285/1‑140 (MQ=255)

                                                                                                               gTCCTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGt                  >  1:581102/1‑140 (MQ=255)

                                                                                                                  cTTCCAATCCGTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGt               <  1:403882/140‑1 (MQ=255)

                                                                                                                            gTTAATGAAGCTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGCCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGtt     >  2:906626/1‑140 (MQ=255)

                                                                                                                               aaTGAAGTTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTggg  >  1:441663/1‑140 (MQ=255)

AGCTGGCGACCAAACGCGCTGCACTGTTGGTGATGCTGTGCTTTTTGCTGGCGGGTTACTGGCTGTGGGTCGGTATTGATGGCTTTGTACTGCTCGCCCAGGATGCTAACGGTCCTTCCAATCCGTTAATGAAACTGGTGGCAGTGCTACCTGGTGCCTGGATGAATAATTTTGTCGAGTCGCCCGTTTTGTGGATCTTCCCGCTGCTGGGATTCTTCTGCCCATTGCTGACGGTGATGGCGATTTATCGTGGTCGCCCGGGTTGGG  >  NC_000913/1039897‑1040163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A