Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	132	61.4	2079690	99.1%	2060972	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,742,612	T→C	T211A (ACA→GCA)	ribC ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,742,612	0	T	C	100.0%	177.5 / NA	48	T211A (ACA→GCA)	ribC	riboflavin synthase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (23/25); total (23/25)

CAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCC  >  NC_000913/1742476‑1742745

cAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGcc                                                                                                                                    >  2:370409/1‑140 (MQ=255)

   gTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTgg                                                                                                                                 >  2:317935/1‑140 (MQ=255)

    tGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGt                                                                                                                                <  2:403319/140‑1 (MQ=255)

      cTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTg                                                                                                                              >  1:967125/1‑140 (MQ=255)

                                tGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCa                                                                                                    >  2:841060/1‑140 (MQ=255)

                                tGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCa                                                                                                    >  1:658130/1‑140 (MQ=255)

                                  gCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGc                                                                                                  >  1:168089/1‑140 (MQ=255)

                                     ggTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGcaca                                                                                               >  1:510688/1‑140 (MQ=255)

                                       tCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACg                                                                                             <  2:127536/140‑1 (MQ=255)

                                       tCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACg                                                                                             <  2:137875/140‑1 (MQ=255)

                                         gtgtTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGtt                                                                                           <  1:854004/140‑1 (MQ=255)

                                          tgtTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTc                                                                                          >  2:571552/1‑140 (MQ=255)

                                            ttCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTa                                                                                        <  2:394991/140‑1 (MQ=255)

                                            ttCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTa                                                                                        <  2:162147/140‑1 (MQ=255)

                                              ctcACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGTTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc                                                                                      <  2:673070/140‑1 (MQ=255)

                                              ctcACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc                                                                                      <  1:910034/140‑1 (MQ=255)

                                              ctcACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc                                                                                      <  1:712736/140‑1 (MQ=255)

                                              ctcACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc                                                                                      <  2:423863/140‑1 (MQ=255)

                                              ctcACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc                                                                                      <  1:293263/140‑1 (MQ=255)

                                              ctcACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTCGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTAcc                                                                                      <  1:721491/140‑1 (MQ=255)

                                                 aCGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCAAACGTTCTACCGTa                                                                                   >  1:841202/1‑140 (MQ=255)

                                                      gtgtTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTAc                                                                              >  1:41098/1‑140 (MQ=255)

                                                              aaCGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCt                                                                      <  2:951806/140‑1 (MQ=255)

                                                               aCGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTg                                                                     <  1:471117/140‑1 (MQ=255)

                                                                 ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTgcg                                                                   >  2:703906/1‑138 (MQ=255)

                                                                 ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAg                                                                   >  2:421245/1‑140 (MQ=255)

                                                                 ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAg                                                                   >  1:335687/1‑140 (MQ=255)

                                                                 ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAg                                                                   >  1:397855/1‑140 (MQ=255)

                                                                 ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAg                                                                   >  2:374356/1‑140 (MQ=255)

                                                                 ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAg                                                                   >  1:860298/1‑140 (MQ=255)

                                                                 ggCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAg                                                                   >  2:135953/1‑140 (MQ=255)

                                                                     ttCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTtg                                                               <  1:65812/140‑1 (MQ=255)

                                                                          gTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGAt                                                          <  2:7914/140‑1 (MQ=255)

                                                                           tGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATc                                                         <  2:658130/140‑1 (MQ=255)

                                                                           tGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCAGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATc                                                         <  1:39701/140‑1 (MQ=255)

                                                                                           aTTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGAcg                                         >  2:410308/1‑140 (MQ=255)

                                                                                                  cGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTgcgc                                  <  2:510688/140‑1 (MQ=255)

                                                                                                           tcCGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGttttt                         >  1:76909/1‑140 (MQ=255)

                                                                                                            ccGGGGGCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGtttttt                        <  1:871524/140‑1 (MQ=255)

                                                                                                                  gCGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTccc                  <  2:771684/140‑1 (MQ=255)

                                                                                                                   cGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCa                 >  2:610663/1‑140 (MQ=255)

                                                                                                                   cGATTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCa                 >  1:472335/1‑140 (MQ=255)

                                                                                                                     aTTTCAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAag               >  1:430409/1‑140 (MQ=255)

                                                                                                                         cAGATCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTc           <  1:527246/140‑1 (MQ=255)

                                                                                                                            aTCAGGCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTg        <  1:421245/140‑1 (MQ=255)

                                                                                                                                ggCTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGCGCGtt    >  2:1032711/1‑140 (MQ=255)

                                                                                                                                  cTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTcc  <  2:625681/140‑1 (MQ=255)

                                                                                                                                  cTTCTGCGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAACACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTcc  <  1:414125/140‑1 (MQ=255)

CAGGTGCTTTCCGCGCCCGTGATATTCAGCTCTGGCAGGTCGTGTTCTCACGCGGTGTTGAAAACGGCCTTCGAGTGGCTCGCTAAAGGCTATTCTATCGCCCCCTCTCCGGGGGCGATTTCAGATCAGGCTTCTGTGCCTGGTTGATTCATGGCATTTTCTCGTGCCGCCAGCACACGTTCTACCGTATCTACCACTGCCTGAGTTTGTGGATCGATTTCAATGTTGACGCGTGCGCCAAGTTTTTTCTTCCCAAGAGTCGTGCGTTCC  >  NC_000913/1742476‑1742745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A